Results 111 to 120 of about 256 (152)
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Nuclear Import in Digitonin‐Permeabilized Cells
Current Protocols in Cell Biology, 2000AbstractImport of proteins into the nucleus of a cell is a complex process that can be reconstituted in vitro. Digitonin‐permeabilized cells are washed free of cytosolic factors to provide competent nuclei. Cytosolic factors for import are provided by an extract of Xenopus ovarian cells.
M S, Moore, E D, Schwoebel
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Digitonin reaction in electron microscopy
Histochemie, 1968The ultrastructural localization of cholesterol (3β-hydroxysterols) has been studied by the digitonin reaction adapted to the electron microscope. The reaction was undertaken in the adrenal gland, liver, and seminiferous tubules of adult albino rats.
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Sedimentation of Bovine Rhodopsin—Digitonin Micelles
Nature New Biology, 1971RHODOPSIN, the photo-sensitive pigment of vertebrate vision receptors, consists of the lipoprotein opsin bound to the 11-cis isomer of retinal. Light isomerizes the 11-cis configuration to the all-trans, which makes the pigment unstable, leading eventually to the dissociation of the retinal from the lipoprotein. The belief that these dark steps involve
N L, Incardona, K, Miles, B N, Baker
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Influence of temperature on hemolysis of erythrocytes by digitonin
Blut, 1980The kinetics of hemolysis of pig erythrocytes by digitonin was continuously monitored by a potassium selective electrode. The following minimal mechanism of hemolysis was postulated, based upon kinetic measurements: 2 D + E (1)in equilibrium E x D2 (2)leads to (E x D2) (3)leads to (E x D2) where D denotes a digitonin molecule and E a specific digitonin
D, Pericin, V, Leskovac
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The effects of digitonin and glycyrrhizin on liposomes
Archives of Pharmacal Research, 1986Digitonin is a strong hemolysin and glycyrrhizin has protective activity against the deterring effect of other hemolytic saponins. The interaction of these saponins with liposomes was studied as a function of cholesterol in membrane. In the case of multilamellar vesicles, which act as ideal osmometers, digitonin disrupted the barrier function of ...
Byung Sul Yu, Hyun Ok Choi
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Isolation of three isochromic forms of rhodopsin in digitonin
Vision Research, 1977Abstract Rhodopsin can be chromatographically purified by a combination of ECTEOLA-cellulose and an aqueous digitonin solution. The purification procedure separates three fractions of rhodopsin which are spectrally identical but are different in their bleaching kinetics.
H, Shichi +3 more
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Prevention of hypercholesterolemia in monkeys (Macaca fascicularis) by digitonin
The American Journal of Clinical Nutrition, 1978Digitonin mixed in the diet was well tolerated by rats and cynomolgus monkeys (Macaca fascicularis), and prevented the expected rise in plasma cholesterol in monkeys fed a diet containing butter and cholesterol. Both digitonin and mildly hydrolyzed digitonin precipitated cholesterol dispersed in micellar suspensions and decreased intestinal absorption ...
M R, Malinow, P, McLaughlin, C, Stafford
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Nuclear import of DNA in digitonin-permeabilized cells
Journal of Cell Science, 1997ABSTRACT DNA can enter intact mammalian nuclei with varying degrees of efficiency in both transfected and microinjected cells, yet very little is known about the mechanism by which it crosses the nuclear membrane. Nucleocytoplasmic transport of fluorescently labeled DNA was studied using a digitonin-permeabilized cell system.
J E, Hagstrom +5 more
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Exchange of adenine nucleotides in digitonin-treated mitochondria
Biochimica et Biophysica Acta (BBA) - Bioenergetics, 1970Abstract 1. 1. The exchange of adenine nucleotides has been studied in phosphorylating inner membrane preparations of rat-liver mitochondria prepared by treatment with digitonin. 2. 2. The digitonin treatment removes nearly 75% of the outer membrane, as seen by the loss of rotenone-insensitive NADH cytochrome c reductase, while the activity ...
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