Results 101 to 110 of about 256 (152)
Effect of Digitonin on Cellular Division
CYTOLOGIA, 1973 Olah, L. V., Hanzely, L.
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Effect of Digitonin on Solubility of Phospholipids
Experimental Biology and Medicine, 1941 H. D. McEwen, P. L. MacLachlan
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Effect of Digitonin on Cellular Division
CYTOLOGIA, 1972 Olah, L. V., Bozzola, J. J.
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Effect of Digitonin on Cellular Division
CYTOLOGIA, 1968 Underbrink, A. G., Olah, L. V.
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Biochimica Et Biophysica Acta - Biomembranes, 1982
We describe a radioimmunoassay for digitonin which utilizes the ability of digotonin to compete with 125I-labeled digoxigenin for binding to anti-digoxin antiserum. As performed using the Gammaflo automated radioimmunoassay system and commercially available reagents, the assay can detect as little as 20 micrograms/ml (15 microM) digitonin. The assay is
E M Ross, G Brooker
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We describe a radioimmunoassay for digitonin which utilizes the ability of digotonin to compete with 125I-labeled digoxigenin for binding to anti-digoxin antiserum. As performed using the Gammaflo automated radioimmunoassay system and commercially available reagents, the assay can detect as little as 20 micrograms/ml (15 microM) digitonin. The assay is
E M Ross, G Brooker
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Interactions between digitonin and bilayer membranes
Biochimica Et Biophysica Acta - Biomembranes, 1984The morphology of interactions between digitonin and cholesterol has been investigated. When precipitated from ethanolic solutions, digitonin-cholesterol complexes form in flat lamellar sheets. In contrast, when the complex is formed in a bilayer membrane, the membrane is deformed into corrugations of hemitubules.
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Disordering Effects of Digitonin on Phospholipid Monolayers
Langmuir, 2017Digitonin, a steroidal saponin obtained from the foxglove plant (Digitalis purpurea), displays a wide spectrum of biological properties and is often used as a model in mechanistic investigations of the biological activity of saponins. In the present study, Langmuir monolayers of zwitterionic (DPPC, DMPE, POPC, POPE, DSPC, DSPE, and DPPE) and ionic ...
M. Orczyk +2 more
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Digitonin‐Permeabilized Cells Are Exocytosis Competent
Journal of Neurochemistry, 1987Abstract:Release of norepinephrine from PC12 cells can be stimulated by free Ca2+ in micromolar concentrations after permeabilization with 10 μg/ml of digitonin. This release is time and temperature dependent, half‐maximal at 0.3 μM Ca2+, and, after washing out of endogenous ATP, half‐ maximal at about 0.5 mA/ MgATP when exogenously added.
Schäfer T +4 more
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Digitonin Effects on Photoreceptor Adenylate Cyclase
Science, 1972Adenylate cyclase is described in a number of photoreceptor membranes. Vertebrate rod outer segments contain light-regulated cyclase, and light regulation is abolished by digitonin. Disruption of microvilli in cone and rhabdom photoreceptors is also associated with loss of light regulation and retention of full enzymic activity.
M W, Bitensky, R E, Gorman, W H, Miller
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Ca2+ transport in digitonin-permeabilized trypanosomatids
Molecular and Biochemical Parasitology, 1990The use of digitonin to permeabilize Leishmania mexicana mexicana, Leishmania agamae, and Crithidia fasciculata plasma membranes enabled us to study Ca2+ transport in situ. The present results show that the mitochondria of these trypanosomatids are able to build up and retain a membrane potential as indicated by a tetraphenylphosphonium-sensitive ...
A E, Vercesi +4 more
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