Results 101 to 110 of about 26,012 (183)

Properties of axon membrane as affected by cobra venom, digitonin, and proteases

, 1964
Electrical excitability and electrical constants of the lobster axon membrane have been measured under the influence of externally applied cobra venom, digitonin, or proteases in an attempt to gain insight into the molecular nature of membrane ...
Toshio Narahashi, Julian M. Tobias
core   +1 more source

Interactions of OSW‑1 with Lipid Bilayers in Comparison with Digitonin and Soyasaponin

, 2020
OSW-1, a unique steroidal saponin isolated from the bulbs of Ornithogalum saundersiae, has potent cell-growth inhibition activity.
Kaori Sakurai (1272567), Shinya Hanashima (1686715), Shinya Hanashima, Michio Murata (1343712), Raymond Malabed, Raymond Malabed (4583191), Michio Murata, Kaori Sakurai   +7 more
core   +1 more source

Immunostaining for oligodendrocyte-specific galactosphingolipids in fixed brain sections using the cholesterol-selective detergent digitonin

, 2009
Galactocerebroside (GalC) and its sulfated derivative sulfatide (SUL) are galactosphingolipids abundantly expressed in oligodendrocytes (OLs). Despite their biological importance in OL development and function, attempts to visualize GalC/SUL in tissue ...
Toda, T., Iwai, L., Kawasaki, H., Matsubayashi, Y., Lu, Q.   +4 more
core   +1 more source

Digitonin-Loaded Nanoscale Metal–Organic Framework for Mitochondria-Targeted Radiotherapy-Radiodynamic Therapy and Disulfidptosis [PDF]

The efficacy of radiotherapy (RT) is limited by inefficient X-ray absorption and reactive oxygen species generation, upregulation of immunosuppressive factors, and a reducing tumor microenvironment (TME).
Weichselbaum, Ralph R., Zhen, Wenyao, Lin, Wenbin, Li, Jinhong, Blenko, Abigail L., Germanas, Tomas, Tillman, Langston, Fan, Yingjie   +7 more
core   +1 more source

DIGITONIN PERMEABILIZATION DOES NOT AFFECT MITOCHONDRIAL-FUNCTION AND ALLOWS THE DETERMINATION OF THE MITOCHONDRIAL-MEMBRANE POTENTIAL OF TRYPANOSOMA-CRUZI INSITU

, 2015
Digitonin can be used to permeabilize selectively the plasma membrane of Trypanosoma cruzi epimastigotes without significantly affecting the functional integrity of mitochondria. Addition of digitonin at concentrations close to 64-mu-M caused decrease in
VERCESI, AE, HOFFMANN, ME, GADELHA, FR, BERNARDES, CF, DOCAMPO, R   +4 more
core   +1 more source

Effects of hydroxylamine, digitonin and triton X-100 on photoreceptor (paraflagellarswelling) and photoreception of Euglena gracilis.

, 1993
We present experiments that test the effects of agents commonly used in visual pigment investigations, namely hydroxylamine (NH 2OH), digitonin and triton X-100, on the photoreceptor and photoreception of Euglena. Hydroxylamine reacts with free and opsin-
Dunlap JR, Passarelli V, LENZI, PAOLA, Barsanti L, Gualtieri P., Walne PL   +5 more
core  

Decreased complex V abundance and activity in digitonin-solubilized mitochondria from the icp55Δ strain.

, 2013
Digitonin-solubilized mitochondria were separated on a 4–16% bis-tris gel using blue native gel electrophoresis (A). First lane contains size standards followed by equivalent amounts of mitochondrial protein. High molecular weight bands were quantitated (
Erine M. Stames (468298), John F. O'Toole (267742)   +1 more
core   +1 more source

Effects of trypsin on secretion stimulated by micromolar Ca 2+ and phorbol ester in digitonin-permeabilized adrenal chromaffin cells

, 1988
1. Catecholamine secretion from digitonin-treated chromaffin cells is stimulated directly by micromolar Ca 2+ in the medium. The permeabilized cells are leaky to proteins.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/44285/1 ...
Senter, Ruth A., Holz, Ronald W.
core   +1 more source

In situ assay of intracellular enzymes of yeast (Kluyveromyces fragilis) by digitonin permeabilization of cell membrane.

, 1988
The yeast, Kluyveromyces fragilis was permeabilized to a number of low-molecular-weight substrates using digitonin. The activities of intracellular yeast enzymes, viz., alcohol dehydrogenase (ADH), beta-galactosidase, glucose-6-phosphate dehydrogenase ...
Bhat, S. G., Gowda, L. R., Joshi, M. S.
core   +1 more source

A fluorescence-based protocol to quantitatively titrate CUT&RUN buffer components

STAR Protocols
Summary: Cleavage under targets & release using nuclease (CUT&RUN) is a technique for identifying genomic sites where proteins or histone modifications are present in chromatin in permeabilized cells.
Andrew Katznelson, Kenneth Zaret
doaj   +1 more source