Results 61 to 70 of about 279,150 (304)

The role of miR‐335‐5p in the redifferentiation of BRAF p.V600E thyroid cancers

Molecular Oncology, EarlyView.
The BRAF p.V600E mutation promotes thyroid cancer dedifferentiation and radioiodine resistance. Using a network approach, we identified miR‐335‐5p as a key regulator of BRAF‐mutated thyroid tumors. Restoring miR‐335‐5p increased thyroid‐specific gene expression and iodine uptake in cells and organoids.
Valeria Pecce, Simone Bini, Marialuisa Sponziello, Giorgio Grani, Giulia Fiscon, Paola Paci, Lorenzo Farina, Rosa Falcone, Valentina Maggisano, Sebastiano Filetti, Cosimo Durante, Antonella Verrienti   +11 more
wiley   +1 more source

Hsc70 staining of digitonin permeabilized cells.

, 2012
Hsc70 staining of digitonin permeabilized cells. MEF, TT MEF, MEF-H2 & TT MEF-H2 heated at 44°C 20 min and harvested at specified time points after heating.
Rohan Steel (109615), Robin L. Anderson (109618), Sarah L. Ellis (109617), Ryan S. Cross (109616)   +3 more
core   +1 more source

Sequestration of Muscarinic Cholinergic Receptors in Permeabilized Neuroblastoma Cells [PDF]

, 1994
The feasibility of using a permeabilized preparation of human SH-SY-5Y neuroblastoma cells for studies of muscarinic acetylcholine receptor (mAChR) sequestration has been evaluated. Exposure of cells permeabilized with digitonin, streptolysin-O, or the Α-
Diana M. Slowiejko, Slowiejko, Diana M., Allan I. Levey, Stephen K. Fisher, Fisher, Stephen K., Levey, Allan I.   +5 more
core   +1 more source

Annexin A6 mediates calcium-dependent exosome secretion during plasma membrane repair

eLife, 2023
Exosomes are an extracellular vesicle (EV) subtype that is secreted upon the fusion of multivesicular bodies (MVBs) with the plasma membrane. Exosomes may participate in intercellular communication and have utility as disease biomarkers; however, little ...
Justin Krish Williams, Jordan Matthew Ngo, Isabelle Madeline Lehman, Randy Schekman   +3 more
doaj   +1 more source

Dimethyl fumarate combined with cisplatin at subcytotoxic doses sensitizes cervical cancer toward ferroptosis and apoptosis through GSH restriction and p53 (re)activation

Molecular Oncology, EarlyView.
Dimethyl fumarate (DMF) reduces growth of HPV‐positive cervical cancer spheroids and induces ferroptosis in cervical cancer cells via blocking SLC7A11/Glutathione (GSH) axis. Combination of subcytotoxic doses of DMF and cisplatin (CDDP) further suppresses spheroid growth and drives cell death in 2D culture models.
Carolina Punziano, Giuseppina Minopoli, Simona Romano, Laura Marrone, Katia Aquilano, Maria Lina Tornesello, Raffaella Faraonio   +6 more
wiley   +1 more source

Identification of α2 AR and REEP expression in permeabilized and non-permeabilized cells.

, 2013
HEK293A cells were transfected with HA-α2A AR, HA-α2C AR, Flag-REEP1, Flag-REEP2, or Flag–REEP6 cDNA. Forty-eight hrs post-transfections, cells were fixed with 4% PFA, under permeabilized and non-permeabilized conditions, and examined by wide field ...
Carl M. Hurt (465560), Vincent K. Ho (465561), Susann Björk (465559), Timothy Angelotti (465562)   +3 more
core   +1 more source

Nuclear cross-reactivity of the mouse anti-FLAG antibody is only seen in permeabilized cells.

, 2021
Nuclear cross-reactivity of the mouse anti-FLAG antibody was not seen in non-permeabilized cells, supporting that the sample preparation steps did not inadvertently permeabilize samples, and further supporting the surface staining patterns seen in non ...
Katherine S. Ralston (11768120), Charles G. Barbieri (11768114), Tammie S. Y. Tam (11768117), Wesley Huang (6373970), Rene L. Suleiman (11768108), Samuel S. Hunter (10099278), Hannah W. Miller (3716773), Akhila Bettadapur (11768105), Maura C. Ruyechan (11768111), Matthew L. Settles (9019334)   +9 more
core   +1 more source

Protocol to study internalization and localization dynamics of exogenously added proteins in detergent-permeabilized human cells

STAR Protocols
Summary: Cell-permeabilization and resealing assays are essential tools for studying the localization and functional roles of exogenous factors in detergent-permeabilized cells.
Sritama Ray, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
doaj   +1 more source

CCDC80 suppresses high‐grade serous ovarian cancer migration via negative regulation of B7‐H3

Molecular Oncology, EarlyView.
PAX8 is a lineage‐specific master regulator of transcription in high‐grade serous ovarian cancer (HGSC) progression. We show for the first time that PAX8 facilitates proliferation and metastasis by repressing the cell autonomous tumor suppressor CCDC80 and inducing B7‐H3 expression.
Aya Saleh, Nitzan Medina‐Itzhaki, Milena Chekov, Eden Gal‐Swisa, Roba Gabesh‐Wahabi, Inbar Savyon, Inna Naroditsky, Hilary A. Kenny, Basem Fares, Lina Korsensky, Einav Girsh, Liron Berger, Ruth Perets   +12 more
wiley   +1 more source

Glucocorticoids rapidly inhibit oxytocin-stimulated adrenocorticotropin release from rat anterior pituitary cells, without modifying intracellular calcium transients [PDF]

, 1993
Glucocorticoid hormones suppress the secretion of ACTH evoked by secretagogues such as CRF and arginine vasopressin. In this study, we investigated the effects of glucocorticoids on ACTH release induced by oxytocin (OT) and on intracellular free calcium ...
Link, H., Dayanithi, G., Gratzl, Manfred
core