Results 41 to 50 of about 4,704,619 (300)
Evaluation of Cell Membrane Electropermeabilization by Means of a Nonpermeant Cytotoxic Agent
BioTechniques, 2000 For the evaluation of cell membrane electropermeabilization, cells are usually exposed to electric pulses in the presence of propidium iodide, a fluorescent dye activated by binding to cellular DNA.
Tadej Kotnik +3 more
doaj +1 more source
Concerted action of human chorionic gonadotropin and norepinephrine on intracellular-free calcium in human granulosa-lutein cells [PDF]
, 1993 Luteal cells are known to possess receptors for LH/hCG and receptors of the beta-adrenergic type. Interactions of specific agonists with either receptor lead to the activation of adenylate cyclase and subsequently to an increase of cAMP.
Föhr, K. J. +5 more
core +1 more source
FEBS Letters, EarlyView.This study reveals that the small GTPase Rab14 is necessary for human papillomavirus (HPV) infection and plays an essential role in the transport of virions to the trans‐Golgi network (TGN). HPV in the early endosome (EE), which harbors GTP‐bound Rab14, is transported to the TGN through the switch of Rab14 from its GTP‐bound to GDP‐bound form.
Yoshiyuki Ishii, Iwao Kukimoto
wiley +1 more source
Glucocorticoids rapidly inhibit oxytocin-stimulated adrenocorticotropin release from rat anterior pituitary cells, without modifying intracellular calcium transients [PDF]
, 1993 Glucocorticoid hormones suppress the secretion of ACTH evoked by secretagogues such as CRF and arginine vasopressin. In this study, we investigated the effects of glucocorticoids on ACTH release induced by oxytocin (OT) and on intracellular free calcium ...
Link, H., Dayanithi, G., Gratzl, Manfred
core +1 more source
Determination of the H+-ATP Synthase and Hydrolytic Activities
Bio-Protocol, 2016 The H+-ATP synthase of the inner mitochondrial membrane utilizes the proton gradient generated by the respiratory chain to synthesize ATP. Under depolarizing conditions, it can function in reverse by hydrolyzing ATP to generate a proton gradient.
Javier García-Bermúdez +2 more
doaj +1 more source
Frontiers in Catalysis, 2022 The implementation of biocatalytic steroid hydroxylation processes at an industrial scale still suffers from low conversion rates. In this study, we selected variants of the self-sufficient cytochrome P450 monooxygenase BM3 from Bacillus megaterium (BM3)
Carolin Bertelmann +4 more
doaj +1 more source
Guanylate cyclase activity in permeabilized Dictyostelium discoideum cells [PDF]
, 1996 Dictyostelium discoideum cells respond to chemoattractants by transient activation of guanylate cyclase. Cyclic GMP is a second messenger that transduces the chemotactic signal. We used an electropermeabilized cell system to investigate the regulation of
Schulkes, C.C.G.M. +3 more
core +2 more sources
Structure‐forward targeting of claudins with synthetic binders
FEBS Letters, EarlyView.Claudins form the paracellular barriers between epithelial and endothelial tissues at tight junctions and are targets for molecular binders with the goal of modulating barrier permeability. Claudin‐binding molecules are relevant in drug delivery or in altering claudin interactions with disease‐causing proteins.
Alex J. Vecchio
wiley +1 more source
A STUDY ON THE B-GALACTOSIDASE SPECIFIC ACTIVITY OF PERMEABILIZED CELLS OF KLEUYVEROMYCES FRAGILIS [PDF]
Journal of Veterinary and Animal Sciences, 2005 The B-galactosidase specific activity of permeabilized cells of Kleuyvero»tyces fragilis is placed on ...
R. Geetha , V. Prasad
doaj
Pathogens, 2023 Trypanosoma brucei is the causative agent of African trypanosomiasis, a deadly disease that affects humans and cattle. There are very few drugs to treat it, and there is evidence of mounting resistance, raising the need for new drug development. Here, we
Núria W. Negrão +6 more
doaj +1 more source