Results 41 to 50 of about 279,150 (304)

High metabolic substrate load induces mitochondrial dysfunction in rat skeletal muscle microvascular endothelial cells

open access: yesPhysiological Reports, 2021
The influence of glucose and palmitic acid (PA) on mitochondrial respiration and emission of hydrogen peroxide (H2O2) was determined in skeletal muscle‐derived microvascular endothelial cells. Measurements were assessed in intact and permeabilized (cells
Camilla Hansen   +5 more
doaj   +1 more source

Osmotically sensitive renin release from permeabilized juxtaglomerular cells

open access: yes, 1993
Renin secretion from juxtaglomerular (JG) cells is sensitive to external osmolality in a way that has been suggested to depend either on cellular volume or on effects on secretory granules.
B. L. Jensen, O. Skott
core   +3 more sources

Determination of the H+-ATP Synthase and Hydrolytic Activities

open access: yesBio-Protocol, 2016
The H+-ATP synthase of the inner mitochondrial membrane utilizes the proton gradient generated by the respiratory chain to synthesize ATP. Under depolarizing conditions, it can function in reverse by hydrolyzing ATP to generate a proton gradient.
Javier García-Bermúdez   +2 more
doaj   +1 more source

Hyperosmotic stress induces PARP1‐mediated HPF1‐dependent mono(ADP‐ribosyl)ation

open access: yesFEBS Letters, EarlyView.
Sorbitol‐induced hyperosmotic stress rapidly induces reversible mono(ADP‐ribosyl)ation (MARylation) on PARP1 without the signs of genotoxic signaling. We show that PARP1 autoMARylation is HPF1 dependent and forms hydroxylamine‐resistant O‐glycosidic linkages.
Anna Georgina Kopasz   +11 more
wiley   +1 more source

Evaluation of Cell Membrane Electropermeabilization by Means of a Nonpermeant Cytotoxic Agent

open access: yesBioTechniques, 2000
For the evaluation of cell membrane electropermeabilization, cells are usually exposed to electric pulses in the presence of propidium iodide, a fluorescent dye activated by binding to cellular DNA.
Tadej Kotnik   +3 more
doaj   +1 more source

Use of the chemical permeabilization process in yeast cells: production of high-activity whole cell biocatalysts

open access: yesBioTechnologia, 2020
Yeast cells are popular microorganisms for use in various bioprocesses because of their ability to produce various enzymes. They are also known for their low price. However, the activity of these biocatalysts is limited by cell membrane diffusion. One of
ILONA TRAWCZYŃSKA
doaj   +1 more source

An X-Domain Phosphoinositide Phospholipase C (PI-PLC-like) of Trypanosoma brucei Has a Surface Localization and Is Essential for Proliferation

open access: yesPathogens, 2023
Trypanosoma brucei is the causative agent of African trypanosomiasis, a deadly disease that affects humans and cattle. There are very few drugs to treat it, and there is evidence of mounting resistance, raising the need for new drug development. Here, we
Núria W. Negrão   +6 more
doaj   +1 more source

Septin 9 PB domains coordinate centrosome positioning and microtubule acetylation to control epithelial polarity

open access: yesFEBS Letters, EarlyView.
Septin 9 polybasic domains couple phosphoinositide‐rich membrane binding to centrosome positioning, Golgi organization, and microtubule acetylation to control epithelial polarity. Their loss disrupts this axis, causing centrosome mispositioning, Golgi fragmentation, reduced microtubule acetylation, and polarity inversion via upregulation of the ...
Ting ting Cai   +4 more
wiley   +1 more source

A STUDY ON THE B-GALACTOSIDASE SPECIFIC ACTIVITY OF PERMEABILIZED CELLS OF KLEUYVEROMYCES FRAGILIS [PDF]

open access: yesJournal of Veterinary and Animal Sciences, 2005
The B-galactosidase specific activity of permeabilized cells of Kleuyvero»tyces fragilis is placed on ...
R. Geetha , V. Prasad
doaj  

Hydrophobic Outer Membrane Pores Boost Testosterone Hydroxylation by Cytochrome P450 BM3 Containing Cells

open access: yesFrontiers in Catalysis, 2022
The implementation of biocatalytic steroid hydroxylation processes at an industrial scale still suffers from low conversion rates. In this study, we selected variants of the self-sufficient cytochrome P450 monooxygenase BM3 from Bacillus megaterium (BM3)
Carolin Bertelmann   +4 more
doaj   +1 more source

Home - About - Disclaimer - Privacy