Results 31 to 40 of about 279,150 (304)

In Vivo Chromatin Structure of the Murine Interleukin-5 Gene Region: A New Intact Cell System

open access: yesBioTechniques, 1996
The study of chromatin involvement in the regulation of gene expression has traditionally required the isolation of nuclei. However, cell fractionation techniques are subject to redistribution of proteins during the isolation procedure, which prevents ...
Sanie Ymer, David A. Jans
doaj   +1 more source

Physiological levels of cardiolipin acutely affect mitochondrial respiration in vascular smooth muscle cells

open access: yesCurrent Research in Physiology, 2023
Cardiolipin (CL) is a phospholipid molecule found in the inner mitochondrial membrane, where it normally associates with and activates the respiratory complexes.
Deema Galambo, Andreas Bergdahl
doaj   +1 more source

Cа2+ transporting systems in secretory cells of the rat exorbital lacrimal gland. І. Ca2+ pumps of plasma membrane and endoplasmic reticulum

open access: yesБіологічні студії, 2012
The mechanism of secretion by lacrimal glands, the innervation and orientation effects, and the mechanism of transduction of primary agonists are similar to the major salivary glands.
A. B. Kotliarova, V. V. Manko
doaj   +1 more source

Lysosomal membrane permeabilization in cell death [PDF]

open access: yesOncogene, 2008
Mitochondrial outer membrane permeabilization (MOMP) constitutes one of the major checkpoint(s) of apoptotic and necrotic cell death. Recently, the permeabilization of yet another organelle, the lysosome, has been shown to initiate a cell death pathway, in specific circumstances.
Boya, Patricia, Kroemer, Guido
openaire   +3 more sources

Carbachol increases intracellular free calcium concentrations in human granulosa-lutein cells [PDF]

open access: yes, 1992
We investigated whether the stimulation of human granulosa-lutein cells with muscarinic and nicotinic receptor agonists can cause increases in intracellular free calcium (Ca2+), using Fura-2 microfluorimetry.
Föhr, K. J.   +3 more
core   +1 more source

Concerted action of human chorionic gonadotropin and norepinephrine on intracellular-free calcium in human granulosa-lutein cells [PDF]

open access: yes, 1993
Luteal cells are known to possess receptors for LH/hCG and receptors of the beta-adrenergic type. Interactions of specific agonists with either receptor lead to the activation of adenylate cyclase and subsequently to an increase of cAMP.
Föhr, K. J.   +5 more
core   +1 more source

Data_Sheet_1_Analysis of Mitochondrial Calcium Retention Capacity in Cultured Cells: Permeabilized Cells Versus Isolated Mitochondria.PDF

open access: yes, 2021
In response to various pathological stimuli, such as oxidative and energy stress accompanied by high Ca2+, mitochondria undergo permeability transition (PT) leading to the opening of the non-selective PT pores (PTP) in the inner mitochondrial membrane ...
Silvia Fossati (4836396)   +3 more
core   +1 more source

Permeabilization of lymphocytes with polyethylene glycol 1000. Discrimination of permeabilized cells by flow cytometry [PDF]

open access: yesCytometry, 1982
AbstractThe toxicity of polyethylene glycol 1000 (PEG) used similarly as in cell hybridization experiments, has been studied by flow cytometry, measuring the light scattering and fluorescence distributions of PEG‐treated human lymphocytes stained with propidium iodide, fluorescein diacetate and acridine orange (AO).
G, Szabó, A, Kiss, L, Trón
openaire   +2 more sources

A Quantitative Assay to Measure Stress Granule Association of Proteins and Peptides in Semi-permeabilized Human Cells

open access: yesBio-Protocol, 2020
Stress granules (SGs) are membrane-less organelles that form in the cytoplasm through phase separation, in response to diverse stressors. SGs contain translationally stalled mRNAs, proteins involved in translation, and various RNA-binding proteins (RBPs).
Saskia Hutten, Dorothee Dormann
doaj   +1 more source

Guanylate cyclase activity in permeabilized Dictyostelium discoideum cells [PDF]

open access: yes, 1996
Dictyostelium discoideum cells respond to chemoattractants by transient activation of guanylate cyclase. Cyclic GMP is a second messenger that transduces the chemotactic signal. We used an electropermeabilized cell system to investigate the regulation of
Schulkes, C.C.G.M.   +3 more
core   +1 more source

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