Results 31 to 40 of about 4,704,619 (300)

In Vivo Chromatin Structure of the Murine Interleukin-5 Gene Region: A New Intact Cell System

open access: yesBioTechniques, 1996
The study of chromatin involvement in the regulation of gene expression has traditionally required the isolation of nuclei. However, cell fractionation techniques are subject to redistribution of proteins during the isolation procedure, which prevents ...
Sanie Ymer, David A. Jans
doaj   +1 more source

Physiological levels of cardiolipin acutely affect mitochondrial respiration in vascular smooth muscle cells

open access: yesCurrent Research in Physiology, 2023
Cardiolipin (CL) is a phospholipid molecule found in the inner mitochondrial membrane, where it normally associates with and activates the respiratory complexes.
Deema Galambo, Andreas Bergdahl
doaj   +1 more source

Lysosomal membrane permeabilization and cell death [PDF]

open access: yesTraffic, 2018
Lysosomes are membrane‐enclosed organelles that mediate the intracellular degradation of macromolecules. They play an essential role in calcium regulation and have emerged as key signaling hubs in controlling the nutrient response. Maintaining lysosomal integrity and function is therefore crucial for cellular homeostasis.
Fengjuan Wang   +2 more
openaire   +2 more sources

Lysosomal membrane permeabilization in cell death [PDF]

open access: yesOncogene, 2008
Mitochondrial outer membrane permeabilization (MOMP) constitutes one of the major checkpoint(s) of apoptotic and necrotic cell death. Recently, the permeabilization of yet another organelle, the lysosome, has been shown to initiate a cell death pathway, in specific circumstances.
Boya, Patricia, Kroemer, Guido
openaire   +3 more sources

Carbachol increases intracellular free calcium concentrations in human granulosa-lutein cells [PDF]

open access: yes, 1992
We investigated whether the stimulation of human granulosa-lutein cells with muscarinic and nicotinic receptor agonists can cause increases in intracellular free calcium (Ca2+), using Fura-2 microfluorimetry.
Föhr, K. J.   +3 more
core   +1 more source

A Quantitative Assay to Measure Stress Granule Association of Proteins and Peptides in Semi-permeabilized Human Cells

open access: yesBio-Protocol, 2020
Stress granules (SGs) are membrane-less organelles that form in the cytoplasm through phase separation, in response to diverse stressors. SGs contain translationally stalled mRNAs, proteins involved in translation, and various RNA-binding proteins (RBPs).
Saskia Hutten, Dorothee Dormann
doaj   +1 more source

Permeabilization of lymphocytes with polyethylene glycol 1000. Discrimination of permeabilized cells by flow cytometry [PDF]

open access: yesCytometry, 1982
AbstractThe toxicity of polyethylene glycol 1000 (PEG) used similarly as in cell hybridization experiments, has been studied by flow cytometry, measuring the light scattering and fluorescence distributions of PEG‐treated human lymphocytes stained with propidium iodide, fluorescein diacetate and acridine orange (AO).
G, Szabó, A, Kiss, L, Trón
openaire   +2 more sources

Data_Sheet_1_Analysis of Mitochondrial Calcium Retention Capacity in Cultured Cells: Permeabilized Cells Versus Isolated Mitochondria.PDF

open access: yes, 2021
In response to various pathological stimuli, such as oxidative and energy stress accompanied by high Ca2+, mitochondria undergo permeability transition (PT) leading to the opening of the non-selective PT pores (PTP) in the inner mitochondrial membrane ...
Silvia Fossati (4836396)   +3 more
core   +1 more source

How DASPMI reveals mitochondrial membrane potential : fluorescence decay kinetics and steady-state anisotropy in living cells [PDF]

open access: yes, 2008
Spectroscopic responses of the potentiometric probe 2-(4-(dimethylamino)styryl)-1-methylpyridinium iodide (DASPMI) were investigated in living cells by means of a time- and space-correlated single photon counting technique.
Ramadass, Radhan, Bereiter-Hahn, Jürgen
core   +1 more source

High metabolic substrate load induces mitochondrial dysfunction in rat skeletal muscle microvascular endothelial cells

open access: yesPhysiological Reports, 2021
The influence of glucose and palmitic acid (PA) on mitochondrial respiration and emission of hydrogen peroxide (H2O2) was determined in skeletal muscle‐derived microvascular endothelial cells. Measurements were assessed in intact and permeabilized (cells
Camilla Hansen   +5 more
doaj   +1 more source

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