Nano Select, 2022 Probiotics are greatly exploited for use as biocatalysts to synthesize γ‐aminobutyric acid (GABA). However, the permeability barrier of the cell envelope is considered to be one of the major hindrances in the effective production of GABA.
Divyashri Gangaraju +2 more
doaj +1 more source
Flow-cytometry-based protocol to analyze respiratory chain function in mouse microglia
STAR Protocols, 2022 Summary: Most of the protocols to analyze metabolic features of cell populations from different tissues rely on in vitro cell culture conditions.
Daniel Erny +5 more
doaj +1 more source
Permeabilizing Cell Membranes with Electric Fields [PDF]
Cancers, 2021 The biological impact of exogenous, alternating electric fields (AEFs) and direct-current electric fields has a long history of study, ranging from effects on embryonic development to influences on wound healing. In this article, we focus on the application of electric fields for the treatment of cancers.
Alondra A. Aguilar +7 more
openaire +2 more sources
Experimental substantiation of permeabilized hepatocytes model for investigation of mitochondria in situ respiration [PDF]
The Ukrainian Biochemical Journal, 2015 To verify experimentally the model of permeabilized hepatocytes, the degree of cell permeability was assessed using trypan blue and polarographycally determined cell respiration rate upon succinate (0.35 mM) and α-ketoglutarate (1 mM) oxidation ...
V. M. Merlavsky +3 more
doaj +1 more source
Molecular determinants of large cargo transport into the nucleus
eLife, 2020 Nucleocytoplasmic transport is tightly regulated by the nuclear pore complex (NPC). Among the thousands of molecules that cross the NPC, even very large (>15 nm) cargoes such as pathogens, mRNAs and pre-ribosomes can pass the NPC intact.
Giulia Paci +4 more
doaj +1 more source
Honey bee hemocyte profiling by flow cytometry. [PDF]
PLoS ONE, 2014 Multiple stress factors in honey bees are causing loss of bee colonies worldwide. Several infectious agents of bees are believed to contribute to this problem.
William J Marringa +3 more
doaj +1 more source
PLoS ONE, 2022 PurposeMuscle biopsies are the gold standard to assess mitochondrial respiration; however, biopsies are not always a feasible approach in persons with spinal cord injury (SCI).
Raymond E Lai +5 more
doaj +2 more sources
Cy3-ATP labeling of unfixed, permeabilized mouse hair cells
Scientific Reports, 2021 ATP-utilizing enzymes play key roles in hair bundles, the mechanically sensitive organelles of sensory hair cells in the inner ear. We used a fluorescent ATP analog, EDA-ATP-Cy3 (Cy3-ATP), to label ATP-binding proteins in two different preparations of ...
Itallia V. Pacentine +1 more
doaj +1 more source
Investigation of mitochondrial dysfunction by sequential microplate-based respiration measurements from intact and permeabilized neurons. [PDF]
PLoS ONE, 2012 Mitochondrial dysfunction is a component of many neurodegenerative conditions. Measurement of oxygen consumption from intact neurons enables evaluation of mitochondrial bioenergetics under conditions that are more physiologically realistic compared to ...
Pascaline Clerc, Brian M Polster
doaj +1 more source
D-Allulose Production from D-Fructose by Permeabilized Recombinant Cells of Corynebacterium glutamicum Cells Expressing D-Allulose 3-Epimerase Flavonifractor plautii. [PDF]
PLoS ONE, 2016 A d-allulose 3-epimerase from Flavonifractor plautii was cloned and expressed in Escherichia coli and Corynebacterium glutamicum. The maximum activity of the enzyme purified from recombinant E.
Chul-Soon Park +5 more
doaj +1 more source