Analysis of Mitochondrial Calcium Retention Capacity in Cultured Cells: Permeabilized Cells Versus Isolated Mitochondria [PDF]
Frontiers in Physiology, 2021 In response to various pathological stimuli, such as oxidative and energy stress accompanied by high Ca2+, mitochondria undergo permeability transition (PT) leading to the opening of the non-selective PT pores (PTP) in the inner mitochondrial membrane ...
Sehwan Jang +3 more
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Reconstitution of nuclear import in permeabilized cells. [PDF]
Methods Mol Biol, 2009 The trafficking of protein and RNA cargoes between the cytoplasm and the nucleus of eukaryotic cells, which is a major pathway involved in cell regulation, is mediated by nuclear transport sequences in the cargoes and by shuttling transport factors. The latter include receptors (karyopherins) that recognize the cargoes and carry them across the nuclear
Cassany A, Gerace L.
europepmc +5 more sources
Measuring CPT-1-mediated respiration in permeabilized cells and isolated mitochondria [PDF]
STAR Protocols, 2021 Summary: Carnitine palmitoyltransferase-1 (CPT-1) is a rate-controlling enzyme for long-chain fatty acid oxidation. This manuscript provides protocols for measuring CPT-1-mediated respiration in permeabilized, adherent cell monolayers and mitochondria ...
Krista Yang +3 more
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High-Resolution Respirometry for Simultaneous Measurement of Oxygen and Hydrogen Peroxide Fluxes in Permeabilized Cells, Tissue Homogenate and Isolated Mitochondria [PDF]
Biomolecules, 2015 Whereas mitochondria are well established as the source of ATP in oxidative phosphorylation (OXPHOS), it is debated if they are also the major cellular sources of reactive oxygen species (ROS).
Marina Makrecka-Kuka +2 more
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Assessing the limitations of paraformaldehyde fixation for accurate cell surface receptor measurement [PDF]
Frontiers in PharmacologyIntroductionQuantifying cell surface receptors, distinct from the total cellular receptor pool, which includes both intracellular and membrane-bound compartments, is imperative in cell biology and pharmacology.
Emma Bernard +4 more
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Enzymatic galactosylation of phenolic compounds by permeabilized cells of Pantoea anthophila [PDF]
Applied Microbiology and BiotechnologyThe permeabilized cells of two Pantoea anthophila strains (BI 55.2 and BI 69.1) were evaluated for galactosylation of phenolic compounds with different molecular structure (gallic acid, caffeic acid, catechin, phlorizin, puerarin and mangiferin), the ...
Jesús Rosales Briceño +6 more
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Mitochondrial respiration in human peripheral blood mononuclear cells: methodology and influence of permeabilization and storage [PDF]
Frontiers in Molecular BiosciencesHuman peripheral blood mononuclear cells (PBMCs) can be easily sampled from healthy individuals and patients. Density gradient isolation from human blood or leukocyte concentrates yields a mononuclear cell population of mainly lymphocytes, monocytes, and
Shusuke Sekine +15 more
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Microorganisms, 2023 Atmospheric cold plasma (ACP) treatment can reduce bacterial pathogens in foods. Additional reduction in bacterial cells during storage after ACP treatment was previously reported.
Barun Yadav, M. S. Roopesh
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Biochemical Reconstitution of Ca2+-Dependent Exosome Secretion in Permeabilized Mammalian Cells
Bio-Protocol, 2023 Exosomes are a subpopulation of the heterogenous pool of extracellular vesicles that are secreted to the extracellular space. Exosomes have been purported to play a role in intercellular communication and have demonstrated utility as biomarkers for a ...
Jordan Ngo +3 more
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Labyrinthin: A distinct pan-adenocarcinoma diagnostic and immunotherapeutic tumor specific antigen
Heliyon, 2022 Structural analysis and detection of optimal cell surface localization of labyrinthin, a pan-adenocarcinoma target, was studied with respect to adenocarcinoma specificity vs. normal and non-adenocarcinoma cells.
Michael Babich +3 more
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