Results 81 to 90 of about 3,623,148 (283)
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
FEBS Open Bio, EarlyView.We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
The mimR Package for Graphical Modelling in R [PDF]
The mimR package for graphical modelling in R is introduced. We present some facilities of mimR, namely those relating specifying models, editing models, fitting models and doing model search.
Søren Højsgaard
core
Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens
FEBS Open Bio, EarlyView.Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee +3 more
wiley +1 more source
An IMS LD Graphic Editor using the graphs representation for modifying the course structures
Journal of Interactive Media in Education, 2005 Commentary on: Chapter 20: Delivery of Learning Design: the Explor@ System?s Case. (Paquette, Marino, De la Teja, Léonard, & Lundgren-Cayrol, 2005) Abstract: We developed a Web portal, named netUniversité, that enables the teachers to create their ...
Ecaterina Giacomini Pacurar +2 more
doaj +1 more source
High-Dimensional Graphical Model Search with the gRapHD R Package [PDF]
This paper presents the R package gRapHD for efficient selection of high-dimensional undirected graphical models. The package provides tools for selecting trees, forests, and decomposable models minimizing information criteria such as AIC or BIC, and for
Rodrigo Labouriau +2 more
core
FEBS Open Bio, EarlyView.Hyperosmotic stress triggers the relocation of the CFIm complex from the nucleus to the cytoplasm. This shift creates a nuclear ‘stoichiometric bottleneck’, limiting CFIm availability for mRNA processing. Consequently, specific mRNAs like NUDT21 and DICER1 undergo targeted 3′UTR shortening, demonstrating how spatial protein dynamics drive rapid ...
Hitomi Soumiya +2 more
wiley +1 more source
Evaluating the involvement of autolysosomes in the nuclear translocation of fluorescent proteins
FEBS Open Bio, EarlyView.Endogenously expressed fluorescent proteins can be degraded by autophagy and transported to cell nuclei via the nuclear pore complex. But in some cell lines, for example, HeLa cells which are positive for immunoreactivity of a receptor ligand, such as UCN I, in cell nuclei, fusion of autolysosome with the nuclear envelope is involved in the nuclear ...
Keiichi Ikeda
wiley +1 more source
Discover SensorsThe convergence of quantum sensing and organ-on-a-chip (OoC) technologies represents an untapped frontier in biomedical diagnostics and personalised medicine.
Subham Preetam
doaj +1 more source
Open Physics, 2018 In this paper the nonlinear long-short (LS) wave resonance model is analyzed through a new perspective. We obtain the classification of exact solutions by making use of the complete discrimination system for the trial equation method and through the ...
Cimpoiasu Rodica, Pauna Alina Streche
doaj +1 more source