Results 161 to 170 of about 32,826 (199)
Infection and Immunity, 1996 The DNA sequence of the pertussis toxin operon (ptx) of Bordetella pertussis predicts that transcription of the operon ends downstream from the ptxS3 gene at a possible stem-loop structure. Secretion of the assembled pertussis toxin into the culture medium required the expression of 8 genes arranged in an operon (ptl) and lying 55 bp downstream from ...
Ricci S., Rappuoli R., Scarlato V.
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Production of cell mass and pertussis toxin by Bordetella pertussis
Journal of Biotechnology, 1991The cultivation of Bordetella pertussis affects production of pertussis toxin and biomass. Comparison of batch mode, chemostat operation and pHstat-turbidostatic control showed that productivities for the continuous process were greater than that for the batch operation.
P, Licari, G R, Siber, R, Swartz
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Pertussis toxin (Bordetella pertussis)
1997Abstract PT (Sekura et al. 1985) is a protein of 105 000 daltons composed of five noncovalently linked subunits named 51 through 55, and organized into two functional domains called A and B (Tamura et al. 1982). The A domain, which is composed of the 51 subunit, is an enzyme that intoxicates eukaryotic cells by ADP-ribosylating their GTP¬
R Rappuoli, M G Pizza
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Binding of NAD+ to pertussis toxin
Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1991The equilibrium dissociation constant of NAD+ and pertussis toxin was determined by equilibrium dialysis and by the quenching of the protein's intrinsic fluorescence on titration with NAD+. A binding constant, Kd, of 24 +/- 2 microM at 30 degrees C was obtained from equilibrium dialysis, consistent with the previously determined value for the Michaelis
M D, Lobban +2 more
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Effect of Pertussis Toxin on Radioligand Binding to Rat Brain Adenosine A1Receptors
Journal of Neurochemistry, 1992 In a previous study we showed that in vivo treatment with pertussis toxin could inhibit some, but not all, effects of adenosine in the rat hippocampus. In this study we investigated the effect of pertussis toxin on the binding of adenosine analogues to ...
Fiona E Parkinson +1 more
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The effect of pH on the production of pertussis toxin by Bordetella pertussis
Journal of Biotechnology, 1991The production of pertussis toxin by Bordetella pertussis was increased by controlling the pH at 7.0 through the addition of sulfuric acid. The more commonly used hydrochloric acid and Tris buffer were observed to be detrimental to toxin yields.
P, Licari, L, Winberry, R, Swartz
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The role of pertussis toxin in clinical manifestations of pertussis
ToxiconPertussis is an acute respiratory infectious disease caused by Bordetella pertussis (B. pertussis). In recent years, the resurgence of pertussis has once again drawn widespread attention from the academic community and society at large. The pathogenicity of B.
Wenjuan, Zhao +4 more
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