Results 181 to 190 of about 45,181 (210)

PFGE in physical mapping

1995
Abstract The development of pulsed field gel electrophoresis (PFGE), which allows the separation of DNA molecules as large as 10 Mb, has enabled large regions of genomic DNA to be mapped and analysed without cloning the DNA (1–3).
M F Ho, A P Monaco
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Mutation detection and diagnosis Using PFGE

1995
Abstract Pulse field gel electrophoresis (PFGE) was specifically designed to separate large DNA fragments (1). Consequently, the great innovation of PFGE analysis for mutation identification and diagnosis was its range of detection and its ability to scan for genetic rearrangements at large distances from a given locus (probe).
den Dunnen, J.T., Liang, P., van Ommen, G.J.B., Van Broeckhoven, Christine   +3 more
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Rapid PFGE method for fingerprinting of Serratia marcescens isolates

Journal of Microbiological Methods, 2009
We modified the conventional PFGE procedure for Serratia marcescens to establish a rapid method. Our protocol showed modification in the bacterial lysis, washing, and restriction enzyme digestion time. This resulted in shortening the time needed by about 3 days compared to the conventional PFGE method.
Alwaleed, Alaidan, Marie Fe, Bohol, Sahar I, Al-Thawadi, Abdulrahman, Al-Suwaine, Ahmed A, Al-Qahtani, Mohammed N, Al-Ahdal   +5 more
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Localization of yeast glucoamylase genes by PFGE and OFAGE

Current Genetics, 1988
Chromosomes of two closely related yeast strains, the amylolytic Saccharomyces diastaticus and the non-amylolytic Saccharomyces cerevisiae, were resolved by pulsed field gel electrophoresis (PFGE) and orthological field alteration gel electrophoresis (OFAGE). Electrophoretic karyotypes of these two strains are identical.
Pretorius I.S., Marmur J.
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Yeast artificial chromosomes cloning using PFGE

1995
Abstract The major goal of the human genome project includes the isolation of the entire human genome in overlapping clones and the development of physical maps of the cloned DNA. Cloning into yeast artificial chromosomes (YAC) represents the method of choice for genome mapping analysis in the megabase range.
P Ougen, D Cohen
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Construction of Lambda Libraries from Large PFGE Fragments

2003
Pulsed-field gel electrophoresis (PFGE) has the capacity to fractionate large fragments of DNA up to thousands of kilobases in size. This aspect of the technique has been exploited for constructing long-range restriction maps of chromosomes from many different species including humans (see Chapters 14 , 15 , and 18 ).
C, Pritchard, M, Burmeister
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PFGE Protocols to Distinguish Subspecies of Lactococcus lactis

2015
Pulsed-field gel electrophoresis (PFGE), developed in the mid-1980s, rapidly became a "gold standard" method for analyzing bacterial chromosomes. Today, although outcompeted in resolution by alternative methods, such as optical mapping, and not applicable for high-throughput analyses, PFGE remains a valuable method for bacterial strain typing. Here, we
Le Bourgeois, Pascal, Passerini, Delphine, Coddeville, Michèle, Guellerin, Maéva, Daveran-Mingot, Marie-Line, Ritzenthaler, Paul   +5 more
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Pulsed-Field Gel Electrophoresis (PFGE) Analysis of Listeria monocytogenes

2020
PFGE is a valuable tool for assessing L. monocytogenes strain interrelatedness. It is based on the study of total bacterial DNA restriction patterns. Cells are embedded in agarose plugs before being lysed. The released DNA is then digested into large fragments by restriction enzymes.
Karen, Hunt, Kieran, Jordan
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Pulsed-Field Gel Electrophoresis (PFGE) for Pathogenic Cronobacter Species

2015
Pulsed-field gel electrophoresis (PFGE) is a molecular-based subtyping strategy that uses a suitable DNA restriction endonuclease enzyme to cut genomic DNA into several large linear fragments, that can be separated based on their sizes. PFGE has been successfully applied to the subtyping of many pathogenic bacteria, including Cronobacter species, and ...
Qiongqiong, Yan, Séamus, Fanning
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Wilms' tumor‐specific methylation pattern in 11p13 detected by PFGE

Genes, Chromosomes and Cancer, 1992
AbstractThe analysis of 2,550 kb from 11 p 13 in Wilms' tumor (WT) material revealed two regions that differed significantly in their methylation between tumor and normal tissue. In WT a hypomethylated area defined by an Nrul and Mlul recognition site 100–150 kb centromeric of the WT gene WTI (site A) and hypermethylation defined by an Nrul and Sacll ...
B, Royer-Pokora, S, Schneider
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