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Analysis of the genomes of protozoan parasites using PFGE
1995Abstract Pulsed field gel electrophoresis (PFGE) has played a major role in developing our current knowledge of the genomes of a number of protozoan parasites as the genomes are small and the chromosomes usually fall within a usable size range. The studies on malaria and trypanosomatids described here demonstrate the applicability of the
David J Kemp, Roberto Cappai
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Pulsed-Field Gel Electrophoresis (PFGE) Analysis of Listeria monocytogenes
2020PFGE is a valuable tool for assessing L. monocytogenes strain interrelatedness. It is based on the study of total bacterial DNA restriction patterns. Cells are embedded in agarose plugs before being lysed. The released DNA is then digested into large fragments by restriction enzymes.
Karen, Hunt, Kieran, Jordan
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Introduction to Pulsed-Field Gel Electrophoresis (PFGE)
1999An agarose gel is made up of pores of various sizes with net pore size varying with concentration of the matrix. The mobility of a DNA molecule depends on how easily it can pass through these pores. Larger molecules must change their conformation to get through the smaller pores and thus migrate more slowly.
Feng Qian, Gregory G. Germino
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PFGE analysis of yeast artificial chromosomes
1995Abstract There are two basic pulsed field gel electrophoresis (PFGE)-related techniques for mapping yeast artificial chromosomes (YACs). One is to digest the DNA with restriction endonucleases, detect specific fragments with defined probes, and construct a restriction map.
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Proceedings. Symposium on Computer Applications in Medical Care, 1993
Pulse field gel electrophoresis mapping is an important technique for characterizing large segments of DNA. We have developed two tools to aid in the construction of pulse field electrophoresis gel maps: PFGE READER which stores experimental conditions and calculates fragment sizes and PFGE MAPPER which constructs pulse field gel electrophoresis maps.
M A, Shifman, P, Nadkarni, P L, Miller
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Pulse field gel electrophoresis mapping is an important technique for characterizing large segments of DNA. We have developed two tools to aid in the construction of pulse field electrophoresis gel maps: PFGE READER which stores experimental conditions and calculates fragment sizes and PFGE MAPPER which constructs pulse field gel electrophoresis maps.
M A, Shifman, P, Nadkarni, P L, Miller
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A method for overcoming DNA degradation during PFGE for Serratia marcescens
Journal of Microbiological Methods, 2011The DNA of some bacteria is broken up by Tris-dependent endonuclease activity during the process of sample preparation for pulsed field gel electrophoresis (PFGE). Adding thiourea to the electophoresis buffer for isolates that exhibit DNA degradation has been the method used for many bacterial genera.
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Karyotyping by PFGE of clinical isolates of
FEMS Immunology and Medical Microbiology, 1996T TATEISHI +3 more
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Germline rearrangement at the locus detected by PFGE
Cancer Genetics and Cytogenetics, 1992Simon Gayther +4 more
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