Results 181 to 190 of about 32,651 (227)

A Modified Pulsed-Field Gel Electrophoresis (PFGE) Protocol for Subtyping Previously Non-PFGE Typeable Isolates of Clostridium difficile Polymerase Chain Reaction Ribotype 001

Journal of Hospital Infection, 2005
A modified pulsed-field gel electrophoresis (PFGE) protocol was developed and applied to 50 isolates of the UK epidemic strain of Clostridium difficile, polymerase chain reaction (PCR) ribotype 001, to develop a PFGE-based subtyping scheme. This protocol
J S Brazier
exaly   +2 more sources

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Rapid PFGE Method for Fingerprinting of Serratia marcescens Isolates

Journal of Microbiological Methods, 2009
We modified the conventional PFGE procedure for Serratia marcescens to establish a rapid method. Our protocol showed modification in the bacterial lysis, washing, and restriction enzyme digestion time. This resulted in shortening the time needed by about 3 days compared to the conventional PFGE method.
Mohammed N Al-Ahdal, Marie Fe F Bohol, Ahmed Al-Qahtani   +2 more
exaly   +3 more sources

Localization of yeast glucoamylase genes by PFGE and OFAGE

Current Genetics, 1988
Chromosomes of two closely related yeast strains, the amylolytic Saccharomyces diastaticus and the non-amylolytic Saccharomyces cerevisiae, were resolved by pulsed field gel electrophoresis (PFGE) and orthological field alteration gel electrophoresis (OFAGE). Electrophoretic karyotypes of these two strains are identical.
Pretorius I.S., Marmur J.
openaire   +2 more sources

PFGE in physical mapping

1995
Abstract The development of pulsed field gel electrophoresis (PFGE), which allows the separation of DNA molecules as large as 10 Mb, has enabled large regions of genomic DNA to be mapped and analysed without cloning the DNA (1–3).
M F Ho, A P Monaco
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Mutation detection and diagnosis Using PFGE

1995
Abstract Pulse field gel electrophoresis (PFGE) was specifically designed to separate large DNA fragments (1). Consequently, the great innovation of PFGE analysis for mutation identification and diagnosis was its range of detection and its ability to scan for genetic rearrangements at large distances from a given locus (probe).
den Dunnen, J.T., Liang, P., van Ommen, G.J.B., Van Broeckhoven, C.   +3 more
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Physical mapping by PFGE

Methods, 1990
Pulsed-field gel electrophoresis (PFGE) is becoming an increasingly important tool in genetic mapping. The physical maps generated through PFGE techniques complement the classical genetic maps and provide a wider range of markers for mapping. PFGE is also used in conjunction with long-range cloning techniques, especially those based on rare-cutter ...
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A constitutional mutation within the retinoblastoma gene detected by PFGE

Clinical Genetics, 1994
Retinoblastoma may be caused by constitutional mutations in the retinoblastoma gene which segregates as an autosomal dominant inherited predisposition for developing retinoblastoma tumours. Since 75% of these cases are new mutations, there is a need for methods to identify carriers of such germ‐line mutations, so that informed genetic counselling is ...
M, Janson, M, Nordenskjöld
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Construction of Lambda Libraries from Large PFGE Fragments

2003
Pulsed-field gel electrophoresis (PFGE) has the capacity to fractionate large fragments of DNA up to thousands of kilobases in size. This aspect of the technique has been exploited for constructing long-range restriction maps of chromosomes from many different species including humans (see Chapters 14 , 15 , and 18 ).
C, Pritchard, M, Burmeister
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Wilms' tumor‐specific methylation pattern in 11p13 detected by PFGE

Genes, Chromosomes and Cancer, 1992
AbstractThe analysis of 2,550 kb from 11 p 13 in Wilms' tumor (WT) material revealed two regions that differed significantly in their methylation between tumor and normal tissue. In WT a hypomethylated area defined by an Nrul and Mlul recognition site 100–150 kb centromeric of the WT gene WTI (site A) and hypermethylation defined by an Nrul and Sacll ...
B, Royer-Pokora, S, Schneider
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Introduction to Pulsed-Field Gel Electrophoresis (PFGE)

1999
An agarose gel is made up of pores of various sizes with net pore size varying with concentration of the matrix. The mobility of a DNA molecule depends on how easily it can pass through these pores. Larger molecules must change their conformation to get through the smaller pores and thus migrate more slowly.
Feng Qian, Gregory G. Germino
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