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PFGE Protocols to Distinguish Subspecies of Lactococcus lactis [PDF]
, 2015 Pulsed-field gel electrophoresis (PFGE), developed in the mid-1980s, rapidly became a "gold standard" method for analyzing bacterial chromosomes. Today, although outcompeted in resolution by alternative methods, such as optical mapping, and not applicable for high-throughput analyses, PFGE remains a valuable method for bacterial strain typing. Here, we
Le Bourgeois, Pascal +5 more
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A modified pulsed-field gel electrophoresis (PFGE) protocol for subtyping previously non-PFGE typeable isolates of Clostridium difficile polymerase chain reaction ribotype 001 [PDF]
Journal of Hospital Infection, 2005 A modified pulsed-field gel electrophoresis (PFGE) protocol was developed and applied to 50 isolates of the UK epidemic strain of Clostridium difficile, polymerase chain reaction (PCR) ribotype 001, to develop a PFGE-based subtyping scheme. This protocol
Jon S Brazier
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Localization of yeast glucoamylase genes by PFGE and OFAGE
Current Genetics, 1988Chromosomes of two closely related yeast strains, the amylolytic Saccharomyces diastaticus and the non-amylolytic Saccharomyces cerevisiae, were resolved by pulsed field gel electrophoresis (PFGE) and orthological field alteration gel electrophoresis (OFAGE). Electrophoretic karyotypes of these two strains are identical.
Pretorius I.S., Marmur J.
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1995
Abstract The development of pulsed field gel electrophoresis (PFGE), which allows the separation of DNA molecules as large as 10 Mb, has enabled large regions of genomic DNA to be mapped and analysed without cloning the DNA (1–3).
M F Ho, A P Monaco
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Abstract The development of pulsed field gel electrophoresis (PFGE), which allows the separation of DNA molecules as large as 10 Mb, has enabled large regions of genomic DNA to be mapped and analysed without cloning the DNA (1–3).
M F Ho, A P Monaco
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Mutation detection and diagnosis Using PFGE
1995Abstract Pulse field gel electrophoresis (PFGE) was specifically designed to separate large DNA fragments (1). Consequently, the great innovation of PFGE analysis for mutation identification and diagnosis was its range of detection and its ability to scan for genetic rearrangements at large distances from a given locus (probe).
den Dunnen, J.T. +3 more
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Methods, 1990
Pulsed-field gel electrophoresis (PFGE) is becoming an increasingly important tool in genetic mapping. The physical maps generated through PFGE techniques complement the classical genetic maps and provide a wider range of markers for mapping. PFGE is also used in conjunction with long-range cloning techniques, especially those based on rare-cutter ...
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Pulsed-field gel electrophoresis (PFGE) is becoming an increasingly important tool in genetic mapping. The physical maps generated through PFGE techniques complement the classical genetic maps and provide a wider range of markers for mapping. PFGE is also used in conjunction with long-range cloning techniques, especially those based on rare-cutter ...
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A constitutional mutation within the retinoblastoma gene detected by PFGE
Clinical Genetics, 1994Retinoblastoma may be caused by constitutional mutations in the retinoblastoma gene which segregates as an autosomal dominant inherited predisposition for developing retinoblastoma tumours. Since 75% of these cases are new mutations, there is a need for methods to identify carriers of such germ‐line mutations, so that informed genetic counselling is ...
M, Janson, M, Nordenskjöld
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Construction of Lambda Libraries from Large PFGE Fragments
2003Pulsed-field gel electrophoresis (PFGE) has the capacity to fractionate large fragments of DNA up to thousands of kilobases in size. This aspect of the technique has been exploited for constructing long-range restriction maps of chromosomes from many different species including humans (see Chapters 14 , 15 , and 18 ).
C, Pritchard, M, Burmeister
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Wilms' tumor‐specific methylation pattern in 11p13 detected by PFGE
Genes, Chromosomes and Cancer, 1992AbstractThe analysis of 2,550 kb from 11 p 13 in Wilms' tumor (WT) material revealed two regions that differed significantly in their methylation between tumor and normal tissue. In WT a hypomethylated area defined by an Nrul and Mlul recognition site 100–150 kb centromeric of the WT gene WTI (site A) and hypermethylation defined by an Nrul and Sacll ...
B, Royer-Pokora, S, Schneider
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Yeast artificial chromosomes cloning using PFGE
1995Abstract The major goal of the human genome project includes the isolation of the entire human genome in overlapping clones and the development of physical maps of the cloned DNA. Cloning into yeast artificial chromosomes (YAC) represents the method of choice for genome mapping analysis in the megabase range.
P Ougen, D Cohen
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