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Biodegradable Innovations: Harnessing Agriculture for Eco-Friendly Plastics. [PDF]

J Xenobiot
Pandey K, Saharan BS, Singh Y, Sadh PK, Duhan JS, Jabborova D.   +5 more
europepmc   +1 more source

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Biosynthesis of polyhydroxyalkanoates (PHA) by recombinant Ralstonia eutropha and effects of PHA synthase activity on in vivo PHA biosynthesis

International Journal of Biological Macromolecules, 1999
Recombinant strains of Ralstonia eutropha PHB 4, which harbored Aeromonas caviae polyhydroxyalkanoates (PHA) biosynthesis genes under the control of a promoter for R. eutropha phb operon, were examined for PHA production from various alkanoic acids. The recombinants produced poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) [P(3HB-co-3HHx)] from hexanoate ...
Toshiaki Fukui
exaly   +3 more sources

Identification of a new polyhydroxyalkanoate (PHA) producer Aquitalea sp. USM4 (JCM 19919) and characterization of its PHA synthase

Journal of Bioscience and Bioengineering, 2016
Aquitalea sp. USM4 (JCM 19919) was isolated from a freshwater sample at Lata Iskandar Waterfall in Perak, Malaysia. It is a rod-shaped, gram-negative bacterium with high sequence identity (99%) to Aquitalea magnusonii based on 16S rRNA gene analysis. Aquitalea sp. USM4 also possessed a PHA synthase gene (phaC), which had amino acid sequence identity of
Kumar Sudesh
exaly   +3 more sources

Polyhydroxyalkanoate (PHA) synthase genes and PHA-associated gene clusters in Pseudomonas spp. and Janthinobacterium spp. isolated from Antarctica

Journal of Biotechnology, 2020
The polyhydroxyalkanoate (PHA) producing capability of four bacterial strains isolated from Antarctica was reported in a previous study. This study analyzed the PHA synthase genes and the PHA-associated gene clusters from the two antarctic Pseudomonas isolates (UMAB-08 and UMAB-40) and the two antarctic Janthinobacterium isolates (UMAB-56 and UMAB-60 ...
Hua Tiang Tan, Irène Kit Ping Tan, Choon Pin Foong   +2 more
exaly   +4 more sources

Natural and engineered polyhydroxyalkanoate (PHA) synthase: key enzyme in biopolyester production

Applied Microbiology and Biotechnology, 2017
With the finite supply of petroleum and increasing concern with environmental issues associated with their harvest and processing, the development of more eco-friendly, sustainable alternative biopolymers that can effectively fill the role of petro-polymers has become a major focus.
Huibin Zou, Liangzhi Li, Li Liangzhi
exaly   +3 more sources

Polyhydroxyalkanoate (PHA) Homeostasis: The Role of the PHA Synthase

ChemInform, 2003
AbstractFor Abstract see ChemInform Abstract in Full Text.
JoAnne, Stubbe, Jiamin, Tian
openaire   +2 more sources

Class IV polyhydroxyalkanoate (PHA) synthases and PHA-producing Bacillus

Applied Microbiology and Biotechnology, 2015
This review highlights the recent investigations of class IV polyhydroxyalkanoate (PHA) synthases, the newest classification of PHA synthases. Class IV synthases are prevalent in organisms of the Bacillus genus and are composed of a catalytic subunit PhaC (approximately 40 kDa), which has a PhaC box sequence ([GS]-X-C-X-[GA]-G) at the active site, and ...
Takeharu Tsuge, Manami Hyakutake, Kouhei Mizuno   +2 more
openaire   +2 more sources

In vivo evolution of the Aeromonas punctata polyhydroxyalkanoate (PHA) synthase: isolation and characterization of modified PHA synthases with enhanced activity

Applied Microbiology and Biotechnology, 2002
In vivo random mutagenesis of the polyhydroxyalkanoate (PHA) synthase gene from Aeromonas punctata was performed employing the mutator strain Escherichia coli XL1-Red. About 200,000 mutants were screened on Nile red-containing medium and five mutants with enhanced fluorescence were selected. Four of these mutants exhibited enhanced in vivo and in vitro
Amara, AA, Steinbuchel, A, Rehm, BHA
openaire   +3 more sources