Results 141 to 150 of about 5,996 (169)

Biochemical and genetic analysis of PHA synthases and other proteins required for PHA synthesis

International Journal of Biological Macromolecules, 1999
Polyhydroxyalkanoic acids (PHA) represent a complex class of storage polyesters that are synthesized by a wide range of different gram-positive and gram-negative bacteria as well as by some Archaea and that are deposited as insoluble cytoplasmic inclusions.
Rehm, BHA, Steinbuchel, A
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Polyhydroxyalkanoate (PHA) biosynthesis in Thermus thermophilus: Purification and biochemical properties of PHA synthase

Molecular and Cellular Biochemistry, 2003
The biosynthesis of polyhydroxyalkanoates (PHAs) was studied, for the first time, in the thermophilic bacterium Thermus thermophilus. Using sodium gluconate (1.5% w/v) or sodium octanoate (10 mM) as sole carbon sources, PHAs were accumulated to approximately 35 or 40% of the cellular dry weight, respectively.
Anastasia A, Pantazaki, Maria G, Tambaka, Valerie, Langlois, Philippe, Guerin, Dimitrios A, Kyriakidis   +4 more
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Incremental truncation of PHA synthases results in altered product specificity

Enzyme and Microbial Technology, 2012
PHA synthase is the key enzyme involved in the biosynthesis of microbial polymers, polyhydroxyalkanoates (PHA). In this study, we created a hybrid library of PHA synthase gene with different crossover points by an incremental truncation method between the C-terminal fragments of the phaC(Cn) (phaC from Cupriavidus necator) and the N-terminal fragments ...
Qian, Wang, Yongzhen, Xia, Quan, Chen, Qingsheng, Qi   +3 more
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PHA synthase engineering toward superbiocatalysts for custom-made biopolymers

Applied Microbiology and Biotechnology, 2007
Poly-3-hydroxyalkanoates [P(3HA)s] are biologically produced polyesters that have attracted much attention as biodegradable polymers that can be produced from biorenewable resources. These polymers have many attractive properties for use as bulk commodity plastics, fishing lines, and medical uses that are dependent on the repeating unit structures ...
Christopher T, Nomura, Seiichi, Taguchi
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Biosynthesis of Polyhydroxyalkanoate (PHA) Copolymer from Fructose Using Wild‐Type and Laboratory‐Evolved PHA Synthases

Macromolecular Bioscience, 2005
AbstractSummary: Eleven laboratory‐evolved polyhydroxyalkanoate (PHA) synthases which originated from Pseudomonas sp. 61‐3 enzyme (PhaC1Ps), together with the wild‐type enzyme, were applied for PHA synthesis from fructose using Ralstonia eutropha PHB‐4 as a host strain.
Takeharu Tsuge, Kotaro Yano, Shin-ichi Imazu, Keiji Numata, Yoshihiro Kikkawa, Hideki Abe, Seiichi Taguchi, Yoshiharu Doi   +7 more
openaire   +2 more sources

PHA synthase activity controls the molecular weight and polydispersity of polyhydroxybutyrate in vivo

Nature Biotechnology, 1997
A synthetic operon for polyhydroxyalkanoate (PHA) biosynthesis designed to yield high levels of PHA synthase activity in vivo was constructed by positioning a genetic fragment encoding beta-ketothiolase and acetoacetyl-CoA reductase behind a modified synthase gene containing an Escherichia coli promoter and ribosome binding site.
S J, Sim, K D, Snell, S A, Hogan, J, Stubbe, C, Rha, A J, Sinskey   +5 more
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Analysis of polyhydroxyalkanoate (PHA) synthase gene in activated sludge that produces PHA containing 3‐hydroxy‐2‐methylvalerate

Biotechnology and Bioengineering, 2006
AbstractThe identification of phaC which encodes PHA synthase, that is involved in the formation of polyhydroxyalkanoate (PHA) containing 3‐hydroxy‐2‐methylvalerate (3H2MV), was attempted. As of now, PHA containing 3H2MV has been reported to be produced only by mixed microbial cultures in activated sludge, but no pure bacterial cultures.
Atsuko, Michinaka, Jun, Arou, Motoharu, Onuki, Hiroyasu, Satoh, Takashi, Mino   +4 more
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Sequence of PHA synthase gene from two strains of Rhodospirillum rubrum and in vivo substrate specificity of four PHA synthases across two heterologous expression systems

Applied Microbiology and Biotechnology, 2000
A 3.0-kb genomic fragment has been isolated from Rhodospirillum rubrum (ATCC 25903) that contains an open reading frame (ORF) with strong homology to other known polyhydroxyalkanoate (PHA) synthase genes. This ORF has lower homology to the R. rubrum strain Ha PHA synthase than would be expected within the same species.
T, Clemente, D, Shah, M, Tran, D, Stark, S, Padgette, D, Dennis, K, Brückener, A, Steinbüchel, T, Mitsky   +8 more
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Carboxy-terminal modification of polyhydroxyalkanoate (PHA) via alcoholysis reaction catalyzed by Class IV PHA synthase

Polymer Degradation and Stability, 2015
Abstract Polyhydroxyalkanoate (PHA) synthase from the bacterium Bacillus cereus YB-4 (PhaRC YB4 ), a Class IV PHA synthase, shows both PHA polymerization and alcoholysis activities. In PhaRC YB4 -expressing Escherichia coli in the presence of ethanol, biosynthesized PHA is subjected to an ethyl-esterification at the carboxy terminus as a result ...
Manami Hyakutake, Satoshi Tomizawa, Imari Sugahara, Emi Murata, Kouhei Mizuno, Hideki Abe, Takeharu Tsuge   +6 more
openaire   +1 more source

In Vivo Characterization and Application of the PHA Synthase from Azotobacter vinelandii for the Biosynthesis of Polyhydroxyalkanoate Containing 4-Hydroxybutyrate

Polymers, 2021
Nazalan Najimudin, Kumar Sudesh
exaly