Results 171 to 180 of about 194,872 (203)

Class IV polyhydroxyalkanoate (PHA) synthases and PHA-producing Bacillus

Applied Microbiology and Biotechnology, 2015
This review highlights the recent investigations of class IV polyhydroxyalkanoate (PHA) synthases, the newest classification of PHA synthases. Class IV synthases are prevalent in organisms of the Bacillus genus and are composed of a catalytic subunit PhaC (approximately 40 kDa), which has a PhaC box sequence ([GS]-X-C-X-[GA]-G) at the active site, and ...
Takeharu Tsuge, Manami Hyakutake, Kouhei Mizuno   +2 more
openaire   +2 more sources

Biochemical and genetic analysis of PHA synthases and other proteins required for PHA synthesis

International Journal of Biological Macromolecules, 1999
Polyhydroxyalkanoic acids (PHA) represent a complex class of storage polyesters that are synthesized by a wide range of different gram-positive and gram-negative bacteria as well as by some Archaea and that are deposited as insoluble cytoplasmic inclusions.
Rehm, BHA, Steinbuchel, A
openaire   +3 more sources

Polyhydroxyalkanoate (PHA) biosynthesis in Thermus thermophilus: Purification and biochemical properties of PHA synthase

Molecular and Cellular Biochemistry, 2003
The biosynthesis of polyhydroxyalkanoates (PHAs) was studied, for the first time, in the thermophilic bacterium Thermus thermophilus. Using sodium gluconate (1.5% w/v) or sodium octanoate (10 mM) as sole carbon sources, PHAs were accumulated to approximately 35 or 40% of the cellular dry weight, respectively.
Anastasia A, Pantazaki, Maria G, Tambaka, Valerie, Langlois, Philippe, Guerin, Dimitrios A, Kyriakidis   +4 more
openaire   +2 more sources

Identification of a new polyhydroxyalkanoate (PHA) producer Aquitalea sp. USM4 (JCM 19919) and characterization of its PHA synthase

Journal of Bioscience and Bioengineering, 2016
Aquitalea sp. USM4 (JCM 19919) was isolated from a freshwater sample at Lata Iskandar Waterfall in Perak, Malaysia. It is a rod-shaped, gram-negative bacterium with high sequence identity (99%) to Aquitalea magnusonii based on 16S rRNA gene analysis. Aquitalea sp. USM4 also possessed a PHA synthase gene (phaC), which had amino acid sequence identity of
Kumar Sudesh
exaly   +3 more sources

Incremental truncation of PHA synthases results in altered product specificity

Enzyme and Microbial Technology, 2012
PHA synthase is the key enzyme involved in the biosynthesis of microbial polymers, polyhydroxyalkanoates (PHA). In this study, we created a hybrid library of PHA synthase gene with different crossover points by an incremental truncation method between the C-terminal fragments of the phaC(Cn) (phaC from Cupriavidus necator) and the N-terminal fragments ...
Qian, Wang, Yongzhen, Xia, Quan, Chen, Qingsheng, Qi   +3 more
openaire   +2 more sources

PHA synthase engineering toward superbiocatalysts for custom-made biopolymers

Applied Microbiology and Biotechnology, 2007
Poly-3-hydroxyalkanoates [P(3HA)s] are biologically produced polyesters that have attracted much attention as biodegradable polymers that can be produced from biorenewable resources. These polymers have many attractive properties for use as bulk commodity plastics, fishing lines, and medical uses that are dependent on the repeating unit structures ...
Christopher T, Nomura, Seiichi, Taguchi
openaire   +2 more sources

Biosynthesis of Polyhydroxyalkanoate (PHA) Copolymer from Fructose Using Wild‐Type and Laboratory‐Evolved PHA Synthases

Macromolecular Bioscience, 2005
AbstractSummary: Eleven laboratory‐evolved polyhydroxyalkanoate (PHA) synthases which originated from Pseudomonas sp. 61‐3 enzyme (PhaC1Ps), together with the wild‐type enzyme, were applied for PHA synthesis from fructose using Ralstonia eutropha PHB‐4 as a host strain.
Takeharu Tsuge, Kotaro Yano, Shin-ichi Imazu, Keiji Numata, Yoshihiro Kikkawa, Hideki Abe, Seiichi Taguchi, Yoshiharu Doi   +7 more
openaire   +2 more sources

Natural and engineered polyhydroxyalkanoate (PHA) synthase: key enzyme in biopolyester production

Applied Microbiology and Biotechnology, 2017
With the finite supply of petroleum and increasing concern with environmental issues associated with their harvest and processing, the development of more eco-friendly, sustainable alternative biopolymers that can effectively fill the role of petro-polymers has become a major focus.
Mo Xian, Huibin Zou, Liangzhi Li
exaly   +3 more sources

PHA synthase activity controls the molecular weight and polydispersity of polyhydroxybutyrate in vivo

Nature Biotechnology, 1997
A synthetic operon for polyhydroxyalkanoate (PHA) biosynthesis designed to yield high levels of PHA synthase activity in vivo was constructed by positioning a genetic fragment encoding beta-ketothiolase and acetoacetyl-CoA reductase behind a modified synthase gene containing an Escherichia coli promoter and ribosome binding site.
S J, Sim, K D, Snell, S A, Hogan, J, Stubbe, C, Rha, A J, Sinskey   +5 more
openaire   +2 more sources

Analysis of polyhydroxyalkanoate (PHA) synthase gene in activated sludge that produces PHA containing 3‐hydroxy‐2‐methylvalerate

Biotechnology and Bioengineering, 2006
AbstractThe identification of phaC which encodes PHA synthase, that is involved in the formation of polyhydroxyalkanoate (PHA) containing 3‐hydroxy‐2‐methylvalerate (3H2MV), was attempted. As of now, PHA containing 3H2MV has been reported to be produced only by mixed microbial cultures in activated sludge, but no pure bacterial cultures.
Atsuko, Michinaka, Jun, Arou, Motoharu, Onuki, Hiroyasu, Satoh, Takashi, Mino   +4 more
openaire   +2 more sources