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Structural study of PHA synthase from Chromobacterium sp. USM2, PhaCCs
Structural study of PHA synthase from Chromobacterium sp. USM2, PhaCCsopenaire
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International Journal of Biological Macromolecules, 1999
Recombinant strains of Ralstonia eutropha PHB 4, which harbored Aeromonas caviae polyhydroxyalkanoates (PHA) biosynthesis genes under the control of a promoter for R. eutropha phb operon, were examined for PHA production from various alkanoic acids. The recombinants produced poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) [P(3HB-co-3HHx)] from hexanoate ...
Toshiaki Fukui
exaly +3 more sources
Recombinant strains of Ralstonia eutropha PHB 4, which harbored Aeromonas caviae polyhydroxyalkanoates (PHA) biosynthesis genes under the control of a promoter for R. eutropha phb operon, were examined for PHA production from various alkanoic acids. The recombinants produced poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) [P(3HB-co-3HHx)] from hexanoate ...
Toshiaki Fukui
exaly +3 more sources
Biochemical and genetic analysis of PHA synthases and other proteins required for PHA synthesis
International Journal of Biological Macromolecules, 1999Polyhydroxyalkanoic acids (PHA) represent a complex class of storage polyesters that are synthesized by a wide range of different gram-positive and gram-negative bacteria as well as by some Archaea and that are deposited as insoluble cytoplasmic inclusions.
Bernd H A Rehm, Alexander Steinbüchel
exaly +4 more sources
Journal of Biotechnology, 2020
The polyhydroxyalkanoate (PHA) producing capability of four bacterial strains isolated from Antarctica was reported in a previous study. This study analyzed the PHA synthase genes and the PHA-associated gene clusters from the two antarctic Pseudomonas isolates (UMAB-08 and UMAB-40) and the two antarctic Janthinobacterium isolates (UMAB-56 and UMAB-60 ...
Irene Kit Ping Tan +2 more
exaly +4 more sources
The polyhydroxyalkanoate (PHA) producing capability of four bacterial strains isolated from Antarctica was reported in a previous study. This study analyzed the PHA synthase genes and the PHA-associated gene clusters from the two antarctic Pseudomonas isolates (UMAB-08 and UMAB-40) and the two antarctic Janthinobacterium isolates (UMAB-56 and UMAB-60 ...
Irene Kit Ping Tan +2 more
exaly +4 more sources
PHA synthase engineering toward superbiocatalysts for custom-made biopolymers
Applied Microbiology and Biotechnology, 2007Poly-3-hydroxyalkanoates [P(3HA)s] are biologically produced polyesters that have attracted much attention as biodegradable polymers that can be produced from biorenewable resources. These polymers have many attractive properties for use as bulk commodity plastics, fishing lines, and medical uses that are dependent on the repeating unit structures ...
Christopher T Nomura, Seiichi Taguchi
exaly +3 more sources
Polymer Degradation and Stability, 2015
Abstract Polyhydroxyalkanoate (PHA) synthase from the bacterium Bacillus cereus YB-4 (PhaRC YB4 ), a Class IV PHA synthase, shows both PHA polymerization and alcoholysis activities. In PhaRC YB4 -expressing Escherichia coli in the presence of ethanol, biosynthesized PHA is subjected to an ethyl-esterification at the carboxy terminus as a result ...
Kouhei Mizuno +2 more
exaly +2 more sources
Abstract Polyhydroxyalkanoate (PHA) synthase from the bacterium Bacillus cereus YB-4 (PhaRC YB4 ), a Class IV PHA synthase, shows both PHA polymerization and alcoholysis activities. In PhaRC YB4 -expressing Escherichia coli in the presence of ethanol, biosynthesized PHA is subjected to an ethyl-esterification at the carboxy terminus as a result ...
Kouhei Mizuno +2 more
exaly +2 more sources
Natural and engineered polyhydroxyalkanoate (PHA) synthase: key enzyme in biopolyester production
Applied Microbiology and Biotechnology, 2017With the finite supply of petroleum and increasing concern with environmental issues associated with their harvest and processing, the development of more eco-friendly, sustainable alternative biopolymers that can effectively fill the role of petro-polymers has become a major focus.
Huibin Zou, Liang-Zhi Li
exaly +3 more sources
Polyhydroxyalkanoate (PHA) Homeostasis: The Role of the PHA Synthase
ChemInform, 2003AbstractFor Abstract see ChemInform Abstract in Full Text.
JoAnne, Stubbe, Jiamin, Tian
openaire +2 more sources
Class IV polyhydroxyalkanoate (PHA) synthases and PHA-producing Bacillus
Applied Microbiology and Biotechnology, 2015This review highlights the recent investigations of class IV polyhydroxyalkanoate (PHA) synthases, the newest classification of PHA synthases. Class IV synthases are prevalent in organisms of the Bacillus genus and are composed of a catalytic subunit PhaC (approximately 40 kDa), which has a PhaC box sequence ([GS]-X-C-X-[GA]-G) at the active site, and ...
Takeharu Tsuge +2 more
openaire +2 more sources