Results 181 to 190 of about 16,047 (206)

Polyhydroxyalkanoate (PHA) biosynthesis in Thermus thermophilus: Purification and biochemical properties of PHA synthase

Molecular and Cellular Biochemistry, 2003
The biosynthesis of polyhydroxyalkanoates (PHAs) was studied, for the first time, in the thermophilic bacterium Thermus thermophilus. Using sodium gluconate (1.5% w/v) or sodium octanoate (10 mM) as sole carbon sources, PHAs were accumulated to approximately 35 or 40% of the cellular dry weight, respectively.
Anastasia A, Pantazaki, Maria G, Tambaka, Valerie, Langlois, Philippe, Guerin, Dimitrios A, Kyriakidis   +4 more
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In vivo evolution of the Aeromonas punctata polyhydroxyalkanoate (PHA) synthase: isolation and characterization of modified PHA synthases with enhanced activity

Applied Microbiology and Biotechnology, 2002
In vivo random mutagenesis of the polyhydroxyalkanoate (PHA) synthase gene from Aeromonas punctata was performed employing the mutator strain Escherichia coli XL1-Red. About 200,000 mutants were screened on Nile red-containing medium and five mutants with enhanced fluorescence were selected. Four of these mutants exhibited enhanced in vivo and in vitro
Amara, AA, Steinbuchel, A, Rehm, BHA
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Biosynthesis of Polyhydroxyalkanoate (PHA) Copolymer from Fructose Using Wild‐Type and Laboratory‐Evolved PHA Synthases

Macromolecular Bioscience, 2005
AbstractSummary: Eleven laboratory‐evolved polyhydroxyalkanoate (PHA) synthases which originated from Pseudomonas sp. 61‐3 enzyme (PhaC1Ps), together with the wild‐type enzyme, were applied for PHA synthesis from fructose using Ralstonia eutropha PHB‐4 as a host strain.
Takeharu Tsuge, Kotaro Yano, Shin-ichi Imazu, Keiji Numata, Yoshihiro Kikkawa, Hideki Abe, Seiichi Taguchi, Yoshiharu Doi   +7 more
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Incremental truncation of PHA synthases results in altered product specificity

Enzyme and Microbial Technology, 2012
PHA synthase is the key enzyme involved in the biosynthesis of microbial polymers, polyhydroxyalkanoates (PHA). In this study, we created a hybrid library of PHA synthase gene with different crossover points by an incremental truncation method between the C-terminal fragments of the phaC(Cn) (phaC from Cupriavidus necator) and the N-terminal fragments ...
Qian, Wang, Yongzhen, Xia, Quan, Chen, Qingsheng, Qi   +3 more
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Analysis of polyhydroxyalkanoate (PHA) synthase gene in activated sludge that produces PHA containing 3‐hydroxy‐2‐methylvalerate

Biotechnology and Bioengineering, 2006
AbstractThe identification of phaC which encodes PHA synthase, that is involved in the formation of polyhydroxyalkanoate (PHA) containing 3‐hydroxy‐2‐methylvalerate (3H2MV), was attempted. As of now, PHA containing 3H2MV has been reported to be produced only by mixed microbial cultures in activated sludge, but no pure bacterial cultures.
Atsuko, Michinaka, Jun, Arou, Motoharu, Onuki, Hiroyasu, Satoh, Takashi, Mino   +4 more
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Sequence of PHA synthase gene from two strains of Rhodospirillum rubrum and in vivo substrate specificity of four PHA synthases across two heterologous expression systems

Applied Microbiology and Biotechnology, 2000
A 3.0-kb genomic fragment has been isolated from Rhodospirillum rubrum (ATCC 25903) that contains an open reading frame (ORF) with strong homology to other known polyhydroxyalkanoate (PHA) synthase genes. This ORF has lower homology to the R. rubrum strain Ha PHA synthase than would be expected within the same species.
T, Clemente, D, Shah, M, Tran, D, Stark, S, Padgette, D, Dennis, K, Brückener, A, Steinbüchel, T, Mitsky   +8 more
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Identification of a new polyhydroxyalkanoate (PHA) producer Aquitalea sp. USM4 (JCM 19919) and characterization of its PHA synthase

Journal of Bioscience and Bioengineering, 2016
Aquitalea sp. USM4 (JCM 19919) was isolated from a freshwater sample at Lata Iskandar Waterfall in Perak, Malaysia. It is a rod-shaped, gram-negative bacterium with high sequence identity (99%) to Aquitalea magnusonii based on 16S rRNA gene analysis. Aquitalea sp. USM4 also possessed a PHA synthase gene (phaC), which had amino acid sequence identity of
Lee-Mei, Ng, Kumar, Sudesh
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Engineering of polyhydroxyalkanoate (PHA) synthase PhaC2Ps of Pseudomonas stutzeri via site-specific mutation for efficient production of PHA copolymers

Applied Microbiology and Biotechnology, 2011
The site-specific mutagenesis for PHA synthase PhaC2(Ps1317) from Pseudomonas stutzeri 1317 was conducted for optimizing production of short-chain-length and medium-chain-length polyhydroxyalkanoates (scl-mcl PHA). Recombinant Ralstonia eutropha PHB-4 harboring double mutated phaC2 (Ps1317) gene (phaC2 (Ps) QKST) produced 42 wt.% PHA content in the ...
Xiao-Wen, Shen, Zhen-Yu, Shi, Ge, Song, Zheng-Jun, Li, Guo-Qiang, Chen   +4 more
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Chemo-enzymatic synthesis of polyhydroxyalkanoate (PHA) incorporating 2-hydroxybutyrate by wild-type class I PHA synthase from Ralstonia eutropha

Applied Microbiology and Biotechnology, 2011
A previously established improved two-phase reaction system has been applied to analyze the substrate specificities and polymerization activities of polyhydroxyalkanoate (PHA) synthases. We first analyzed the substrate specificity of propionate coenzyme A (CoA) transferase and found that 2-hydroxybutyrate (2HB) was converted into its CoA derivative ...
Xuerong, Han, Yasuharu, Satoh, Toshifumi, Satoh, Ken'ichiro, Matsumoto, Toyoji, Kakuchi, Seiichi, Taguchi, Tohru, Dairi, Masanobu, Munekata, Kenji, Tajima   +8 more
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Homology study of two polyhydroxyalkanoate (PHA) synthases from Pseudomonas aureofaciens.

DNA sequence : the journal of DNA sequencing and mapping, 2002
Recently, we have cloned and analyzed two polyhydroxyalkanoate (PHA) synthase genes (phaC1 and phaC2 in the pha cluster) from Pseudomonas aureofaciens. In this report, the deduced amino acid (AA) sequences of PHA synthase 1 and PHA synthase 2 from P. aureofaciens are compared with those from three other bacterial strains (Pseudomonas sp.
F, Umeda, T, Nishikawa, H, Miyasaka, I, Maeda, M, Kawase, K, Yagi   +5 more
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