Results 81 to 90 of about 63,473 (252)
Antibody engineering and phage display technology
The EuroBiotech Journal, 2017 Here we provide our pipeline for antibody discovery process. Starting from de novo isolation from custom made phage display libraries to expression and downstream applications; it allows us to obtain recombinant antibodies with desired properties to vast
Dormeshkin Dmitri Olegovich
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Advanced Science, EarlyView.Given that PEEK chest wall implants induce shear force at the soft tissue‐implant interface due to respiratory motion in the early in vivo post‐implantation stage. We confirmed that this mechanical stimulus triggers the PIEZO1/ITGB1/YAP1 signalling axis in L929 and the STAT6/p‐STAT6 pathway in RAW264.7, which in turn mediates adhesion and migration of ...
Rou Huang +14 more
wiley +1 more source
Engineering Antibodies into Targeted Chimeras: From Recognition Modules to Programmable Degraders
Advanced Science, EarlyView.Antibody can be engineered into diverse lysosome‐targeting chimeras that convert target recognition into active degradation. By coupling antibody binding with recruited receptors, encoded domains, conjugated ligands, or nanoparticle scaffolds, these antibody‐associated targeted chimeras (AbTACs) platforms direct extracellular and membrane proteins ...
Kaige Chen, Quanyin Hu
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Advanced Science, EarlyView.This graphical abstract presents gut microbial cross‐feeding through a Mechanism–Technology–Application framework. It summarizes major resource‐transfer modes, the experimental and computational tools used to establish causality, and potential interventions involving dietary substrates, live biotherapeutic products, and engineered microbes.
Chuankai Sun +7 more
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Virology JournalBackground To develop efficient selection strategies and improve the discovery of promising ligands, it is highly desirable to analyze the sequence composition of naïve phage display libraries and monitor the evolution of their peptide content during ...
Anders Wilgaard Sinkjaer +5 more
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Monoclonal Antibody Development for Cancer Treatment Using the Phage Display Library Platform
BiologicsThirty-four years ago, the groundbreaking work of John McCafferty and Sir Gregory Winter in developing phage display technology revolutionized the discovery of human antibodies, paving the way for diverse applications.
Tiantian Zhang, Zhe Wang
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Compact 9dBEs Enable Efficient and Precise Genome Editing in Mammalian Cells and In Vivo
Advanced Science, EarlyView.As a compact type II‐D system, the Cas9d‐based platform holds great potential for in vivo applications. Through rational engineering, its derived base editors (9dBEs) enable efficient disease modeling while facilitating single‐vector AAV delivery for in vivo genome editing. These miniature tools offer a robust strategy for basic research and biomedical
Qingquan Xiao +12 more
wiley +1 more source
Angewandte Chemie, EarlyView.An optimized strategy for the ribosomal synthesis of thioisoindole‐bridged bicyclic (TiB) peptides is incorporated into the RaPID platform, enabling high‐throughput discovery from topologically defined TiB libraries. Proof‐of‐concept selection against TNIK identifies nanomolar‐affinity ligands with inhibitory activity, while x‐ray crystallography ...
Yue Zhang +5 more
wiley +2 more sources
Advanced Science, EarlyView.Streptococcal mannose phosphotransferase system component IID (Man‐PTSIID) is identified as a novel RANK‐binding osteoclastogenic factor. By directly binding to RANK and activating NF‐κB independently of TLR2, Man‐PTSIID drives osteoclastogenesis and inflammatory bone destruction, uncovering an unexpected microbial mechanism underlying streptococcal ...
Chaeyeon Park +13 more
wiley +1 more source