Results 41 to 50 of about 125,595 (262)

Genome Sequence of Gordonia Phage BetterKatz [PDF]

Genome Announcements, 2016
ABSTRACT BetterKatz is a bacteriophage isolated from a soil sample collected in Pittsburgh, Pennsylvania using the host Gordonia terrae 3612. BetterKatz's genome is 50,636 bp long and contains 75 predicted protein-coding genes, 35 of which have been assigned putative functions.
Pope, Welkin H., Berryman, Emily N., Forrest, Kaitlyn M., McHale, Lilliana, Wertz, Anthony T., Zhuang, Zenas, Kasturiarachi, Naomi S., Pressimone, Catherine A., Schiebel, Johnathon G., Furbee, Emily C., Grubb, Sarah R., Warner, Marcie H., Montgomery, Matthew T., Garlena, Rebecca A., Russell, Daniel A., Jacobs-Sera, Deborah, Hatfull, Graham F.   +16 more
openaire   +2 more sources

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

The Podovirus ϕ80-18 Targets the Pathogenic American Biotype 1B Strains of Yersinia enterocolitica

Frontiers in Microbiology, 2020
We report here the complete genome sequence and characterization of Yersinia bacteriophage vB_YenP_ϕ80-18. ϕ80-18 was isolated in 1991 using a Y. enterocolitica serotype O:8 strain 8081 as a host from a sewage sample in Turku, Finland, and based on its ...
Karolina Filik, Bożena Szermer-Olearnik, Maciej Wernecki, Lotta J. Happonen, Maria I. Pajunen, Ayesha Nawaz, Muhammad Suleman Qasim, Jin Woo Jun, Laura Mattinen, Mikael Skurnik, Mikael Skurnik, Ewa Brzozowska   +11 more
doaj   +1 more source

Genome Analysis and Antibiofilm Activity of Phage 590B against Multidrug-Resistant and Extensively Drug-Resistant Uropathogenic Escherichia coli Isolates, India

Pathogens, 2022
Urinary tract infections (UTIs) are among the most common bacterial infections in humans. Uropathogenic Escherichia coli (UPEC), which are the most frequent agents causing community as well as hospital-acquired UTIs, have become highly drug-resistant ...
Naveen Chaudhary, Ravi Kumar Maurya, Dharminder Singh, Balvinder Mohan, Neelam Taneja   +4 more
doaj   +1 more source

Evolutionary Stability of a Refactored Phage Genome

ACS Synthetic Biology, 2012
Engineered genetic systems are commonly unstable; if propagated, they evolve to reverse or modify engineered elements because the elements impair fitness. A goal of synthetic biology is thus to anticipate and avoid detrimental engineering, but little is yet known about which types of elements cause problems in different contexts.
Springman, Rachael, Molineux, Ian J., Duong, Chanan, Bull, Robert J., Bull, James J.   +4 more
openaire   +2 more sources

Vibrio choleraePhage K139: Complete Genome Sequence and Comparative Genomics of Related Phages [PDF]

Journal of Bacteriology, 2002
ABSTRACTIn this report, we characterize the complete genome sequence of the temperate phage K139, which morphologically belongs to theMyoviridaephage family (P2 and 186). The prophage genome consists of 33,106 bp, and the overall GC content is 48.9%. Forty-four open reading frames were identified.
Dagmar, Kapfhammer, Julia, Blass, Stefan, Evers, Joachim, Reidl   +3 more
openaire   +2 more sources

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

A reverse transcriptase controls prophage genome reduction to promote phage dissemination in Pseudomonas aeruginosa biofilms

Cell Reports
Summary: Filamentous bacteriophages play a critical role in biofilm formation and virulence in the opportunistic pathogen Pseudomonas aeruginosa. Here, studies of the filamentous Pf4 prophage life cycle within P.
Yunxue Guo, Shituan Lin, Ran Chen, Jiayu Gu, Kaihao Tang, Zhaolong Nie, Zixian Huang, Juehua Weng, Jianzhong Lin, Tianlang Liu, Matthew K. Waldor, Xiaoxue Wang   +11 more
doaj   +1 more source

Genome Characterization of a Novel Wastewater Bacteroides fragilis Bacteriophage (vB_BfrS_23) and its Host GB124

Frontiers in Microbiology, 2020
Bacteroides spp. are part of the human intestinal microbiota but can under some circumstances become clinical pathogens. Phages are a potentially valuable therapeutic treatment option for many pathogens, but phage therapy for pathogenic Bacteroides spp ...
Mohammad A. Tariq, Fiona Newberry, Fiona Newberry, Rik Haagmans, Rik Haagmans, Catherine Booth, Tom Wileman, Tom Wileman, Lesley Hoyles, Martha R. J. Clokie, James Ebdon, Simon R. Carding, Simon R. Carding   +12 more
doaj   +1 more source

Isolation and Characterization of a Newly Discovered Phage, V-YDF132, for Lysing Vibrio harveyi

Viruses, 2022
A newly discovered lytic bacteriophage, V-YDF132, which efficiently infects the pathogenic strain of Vibrio harveyi, was isolated from aquaculture water collected in Yangjiang, China.
Shaozhu Kang, Luhao Zhang, Jiaming Liao, Dongzhuo Zhang, Siting Wu, Xin Zhang, Qiwei Qin, Jingguang Wei   +7 more
doaj   +1 more source