Results 61 to 70 of about 190,753 (310)

HIV‐1 establishes immediate latency in T cells expressing the viral Nef protein

FEBS Open Bio, EarlyView.
Nef is a viral protein often omitted from HIV‐1 reporter viruses. Consequently, its role in viral latency is unclear. We developed three novel dual reporter HIV‐1 derivatives that express Nef and allow for detection of latent and productive infection. Using these reporters, we show that Nef does not affect the establishment of immediate viral latency ...
Cindy Lam, Ivan Sadowski
wiley   +1 more source

A reverse transcriptase controls prophage genome reduction to promote phage dissemination in Pseudomonas aeruginosa biofilms

Cell Reports
Summary: Filamentous bacteriophages play a critical role in biofilm formation and virulence in the opportunistic pathogen Pseudomonas aeruginosa. Here, studies of the filamentous Pf4 prophage life cycle within P.
Yunxue Guo, Shituan Lin, Ran Chen, Jiayu Gu, Kaihao Tang, Zhaolong Nie, Zixian Huang, Juehua Weng, Jianzhong Lin, Tianlang Liu, Matthew K. Waldor, Xiaoxue Wang   +11 more
doaj   +1 more source

Genome Characterization of a Novel Wastewater Bacteroides fragilis Bacteriophage (vB_BfrS_23) and its Host GB124

Frontiers in Microbiology, 2020
Bacteroides spp. are part of the human intestinal microbiota but can under some circumstances become clinical pathogens. Phages are a potentially valuable therapeutic treatment option for many pathogens, but phage therapy for pathogenic Bacteroides spp ...
Mohammad A. Tariq, Fiona Newberry, Fiona Newberry, Rik Haagmans, Rik Haagmans, Catherine Booth, Tom Wileman, Tom Wileman, Lesley Hoyles, Martha R. J. Clokie, James Ebdon, Simon R. Carding, Simon R. Carding   +12 more
doaj   +1 more source

Isolation and Characterization of a Newly Discovered Phage, V-YDF132, for Lysing Vibrio harveyi

Viruses, 2022
A newly discovered lytic bacteriophage, V-YDF132, which efficiently infects the pathogenic strain of Vibrio harveyi, was isolated from aquaculture water collected in Yangjiang, China.
Shaozhu Kang, Luhao Zhang, Jiaming Liao, Dongzhuo Zhang, Siting Wu, Xin Zhang, Qiwei Qin, Jingguang Wei   +7 more
doaj   +1 more source

Isolation of a Novel Phage OTooleKemple52 [PDF]

, 2017
A bacteriophage is a virus capable of infecting bacteria like ubiquitous soil-dwelling genus Bacillus. Within the Bacillus genus, there is the “ACT family” made up of B. thuringiensis, B. cereus, and B.
Raymond, Thomas O
core   +1 more source

Characterizing RecA-Independent Induction of Shiga toxin2-encoding Phages by EDTA Treatment [PDF]

, 2013
The bacteriophage life cycle has an important role in Shiga toxin (Stx) expression. The induction of Shiga toxin-encoding phages (Stx phages) increases toxin production as a result of replication of the phage genome, and phage lysis of the host cell also
Imamovic, Lejla, Muniesa Pérez, Ma Teresa   +1 more
core   +2 more sources

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Open-source genomic analysis of Shiga-toxin–producing E. coli O104:H4 [PDF]

, 2011
An outbreak caused by Shiga-toxin–producing Escherichia coli O104:H4 occurred in Germany in May and June of 2011, with more than 3000 persons infected. Here, we report a cluster of cases associated with a single family and describe an open-source genomic
Antoine Danchin, Cordesmeyer S, Dongfang Li, Fei Pu, Feng Xi, George F. Gao, Holger Rohde, Huanming Yang, Itoh Y, Jian Wang, Jianguo Xu, Jun Oh, Jun Wang, Junhua Li, Junjie Qin, Kuijper E, Liang Yang, Mark J. Pallen, Martin Aepfelbacher, Martin Christner, Meiru Zhao, Moritz Hentschke, Nicholas J. Loman, Peng Wang, Robin Kobbe, Ruifu Yang, Scheutz F, Sebastian Loos, Shenghui Li, Wentong Chen, Xiangna Zhao, Xianwei Yang, Yajun Song, Yangqing Peng, Yin Li, Yingrui Li, Yuanlin Guan, Yujun Cui, Zhaoxi Zhang, Zhong Cen   +39 more
core   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

ΦCrAss001 represents the most abundant bacteriophage family in the human gut and infects Bacteroides intestinalis [PDF]

, 2018
peer-reviewedCrAssphages are an extensive and ubiquitous family of tailed bacteriophages, predicted to infect bacteria of the order Bacteroidales. Despite being found in ~50% of individuals and representing up to 90% of human gut viromes, members of this
Draper, Lorraine A., Fitzgerald, C. Brian, Hill, Colin, Khokhlova, Ekaterina V., Ross, R Paul, Shkoporov, Andrey N., Stockdale, Stephen R.   +6 more
core   +5 more sources