Results 81 to 90 of about 1,452,004 (343)

Meta‐analysis fails to show any correlation between protein abundance and ubiquitination changes

open access: yesFEBS Open Bio, EarlyView.
We analyzed over 50 published proteomics datasets to explore the relationship between protein levels and ubiquitination changes across multiple experimental conditions and biological systems. Although ubiquitination is often associated with protein degradation, our analysis shows that changes in ubiquitination do not globally correlate with changes in ...
Nerea Osinalde   +3 more
wiley   +1 more source

First Order Kaon Condensate

open access: yes, 1998
First order Bose condensation in asymmetric nuclear matter and in neutron stars is studied, with particular reference to kaon condensation. We demonstrate explicitly why the Maxwell construction fails to assure equilibrium in multicomponent substances ...
A. B. Migdal   +41 more
core   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

open access: yesFEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli   +8 more
wiley   +1 more source

Experimental Study of Mixed Gas Hydrates from Gas Feed Containing CH4, CO2 and N2: Phase Equilibrium in the Presence of Excess Water and Gas Exchange

open access: yesEnergies, 2018
This article presents gas hydrate experimental measurements for mixtures containing methane (CH4), carbon dioxide (CO2) and nitrogen (N2) with the aim to better understand the impact of water (H2O) on the phase equilibrium.
Ludovic Nicolas Legoix   +3 more
doaj   +1 more source

Study of the phase transition in the 3d Ising spin glass from out of equilibrium numerical simulations

open access: yes, 2006
Using the decay of the out equilibrium spin-spin correlation function we compute the equilibrium Edward-Anderson order parameter in the three dimensional binary Ising spin glass in the spin glass phase.
A Tarancón   +16 more
core   +3 more sources

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

open access: yesFEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen   +9 more
wiley   +1 more source

Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system

open access: yesFEBS Open Bio, EarlyView.
An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková   +3 more
wiley   +1 more source

Measurement of phase equilibrium characteristics and optimization of state equation for N2-CO2 binary system

open access: yesYou-qi chuyun
Objective The presence of impurities impacts the phase equilibrium of CO2, potentially compromising the safety of CO2 pipeline transmission. As efforts intensify towards advancing the application of Carbon Capture, Utilization and Storage (CCUS ...
Naiya XIE   +5 more
doaj   +1 more source

Reactive Extraction of Lactic Acid, Formic Acid and Acetic Acid from Aqueous Solutions with Tri-n-octylamine/1-Octanol/n-Undecane

open access: yesChemEngineering, 2019
The present work develops the basics for the isolation of lactic acid, acetic acid and formic acid from a single as well as a mixed feed stream, as is present, for example, in fermentation broth for lactic acid production.
Nuttakul Mungma   +2 more
doaj   +1 more source

Large‐scale bidirectional arrayed genetic screens identify OXR1 and EMC4 as modifiers of αSynuclein aggregation

open access: yesFEBS Open Bio, EarlyView.
Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane   +11 more
wiley   +1 more source

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