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Screening for Phenoloxidases from Edible Mushrooms

Bioscience, Biotechnology, and Biochemistry, 2001
A screening test for phenoloxidases from edible mushrooms was done on potato dextrose agar plates that contained phenolic chemicals. Many edible mushrooms showed positive reactions on the agar plates. Among them, Auricularia auricula-judae, Clitocybe nebularis, Lentinus edodes, Pholiota aurivella, and Pseudohiatula oshimae produced a considerable ...
K, Morisaki   +4 more
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Molecular Forms of Apple Phenoloxidase

Biochemie und Physiologie der Pflanzen, 1979
Summary Qualitative differences of dimeric (mol. wt. 60,000—70,000 D) and monomeric (mol. wt. 30,000 to 40,000 D) forms of phenoloxidase (EC 1.10.3.1) isolated from the fruits of two winter apple varieties were studied. It is shown that both forms differ distinctly by k m values and Hill coefficients.
M.N. Zaprometov   +2 more
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Processing of crayfish hemocyanin subunits into phenoloxidase

Biochemical and Biophysical Research Communications, 2004
Hemocyanin and phenoloxidase are both copper-binding proteins involved in the immune system for a wide range of animal species. In crayfish, these proteins were purified and characterized from plasma and hemocytes, respectively. Recently, we have reported that the processing of one of the hemocyanin subunits occurs by a proteolytic cleavage under ...
So Young, Lee   +2 more
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Lipoprotein-induced phenoloxidase-activity in tarantula hemocyanin

Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics, 2015
Phenoloxidases play vital roles in invertebrate innate immune reactions, wound closure and sclerotization processes in arthropods. In chelicerates, where phenoloxidases are lacking, phenoloxidase-activity can be induced in the oxygen carrier hemocyanin in vitro by proteolytic cleavage, incubation with the artificial inducer SDS, or lipids.
Sven, Schenk   +3 more
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Properties of Phenoloxidase in Mycobacterium leprae

Nature, 1968
Mycobacterium leprae separated from infected human tissues, oxidizes 3,4-dihydroxyphenylalanine (dopa) and a few other phenolic compounds1–3. This property is not shown by several other mycobacteria1,2,4. Phenoloxidase occurs in vertebrate melanocytes and is widely distributed in the plant kingdom5. (E.C.
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Phenoloxidase activity of phenol-degrading bacteria

2010 International Conference on Bioinformatics and Biomedical Technology, 2010
In order to study phenoloxidase (PO) activity of the phenol-degrading bacteria, the carbon source of isolation culture medium adopts artificial phenol mixture. Stenotrophomonas maltophilia K279a is isolated from coal chemical industry wastewater. The optimum conditions for PO production are phenol 1000mg/L, temperature 30–34°C and PH 7–7.5.
null Hong-jun Han   +5 more
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Zur Substratspezifität der Phenoloxidase (EC 1.14.18.1)

Acta Histochemica, 1983
Zusammenfassung An 10 µ m dicken Kryostatschnitten von Milz und Darm weiser Ratten wurden im Vergleich mit Dihydroxyphenylalanin folgende Substanzen in jeweils 2 Konzentrationen als Substrat fur das Enzym Phenoloxidase getestet: Pyrogallol, Resorcin, 2,4-Dinitrophenol, Tyraminhydrochlorid, o-Cresol und Adrenalin.
Hans Schmidt, Johannes Koch
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Identification and isolation of endogenous insect phenoloxidase inhibitors

Biochemical and Biophysical Research Communications, 1992
Inhibitors of phenoloxidase were identified in pupae of the housefly, Musca domestica L. The phenoloxidase inhibitors were purified from final instar pupae of the housefly by a combination of ammonium sulfate fractionation, ion-exchange chromatography, gel filtration and reverse-phase high performance liquid chromatography.
T, Tsukamoto   +6 more
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Phenoloxidases in Portabella Mushrooms

Journal of Food Science, 1997
ABSTRACT Tyrosinase, laccase, and peroxidase activities were detected in crude extracts from commercial Portabella mushrooms. Tyrosinase was present in larger amounts than either laccase or peroxidase. Catechol showed the greatest activity as a substrate for tyrosinase while diaminobenzidine was found to be a good substrate for ...
XIAODONG ZHANG, WILLIAM H. FLURKEY
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Phenoloxidase System of the Blowfly, Calliphora erythrocephala

Science, 1964
In Calliphora larvae, an inactive precursor of the enzyme phenoloxidase is present in the hemolymph. A special activator enzyme is localized in the cuticle. When phenoloxidase precursor is allowed to react with the activator, it yields a soluble enzyme having the characteristics mainly of a diphenoloxidase.
C E, SEKERIS, D, MERGENHAGEN
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