Results 131 to 140 of about 6,790 (182)

A Simplified Assay for Cryptococcal Phenoloxidase

Mycologia, 1986
factor (Rhodes et al., 1982). The standard procedure for assaying phenoloxidase activity described by Polacheck et al. (1982) requires extensive sample preparation. Appropriately grown cells must be broken gently by vortexing with glass beads to minimize loss of activity and the membranes collected by ultracentrifugation.
Judith C Rhodes
exaly   +2 more sources

Phenoloxidases in ascidian hemocytes: characterization of the pro-phenoloxidase activating system

Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 2003
The phenoloxidase (PO) activity of the hemocytes lysate supernatant from three ascidians species, assayed by means of 3-methyl-2-benzothiazolinone hydrazone hydrochloride, have been compared. PO-containing hemocytes were identified by a cytochemical reaction and the enzymatic activity measured by a spectrophotometric assay of lysate supernatant from ...
PARRINELLO, Nicolo', ARIZZA, Vincenzo, Chinnici, C, PARRINELLO, Daniela, CAMMARATA, Matteo   +4 more
openaire   +3 more sources

Temperature regulation of the cryptococcal phenoloxidase

Medical Mycology, 1991
Melanin formation at 37 degrees C has been proposed as a virulence factor in Cryptococcus neoformans. However, whereas catecholamine uptake is maintained at this temperature, phenoloxidase, which catalyses the oxidation of catecholamine to melanin, is severely decreased in most wild type strains cultivated at 37 degrees C.
E S, Jacobson, H S, Emery
openaire   +2 more sources

Phenoloxidase of Mycobacterium leprae

Nature, 1967
INVESTIGATIONS of the metabolism of Mycobacteriumleprae1 have revealed a few of the metabolic characteristics of this micro-organism. Concentrates of M. leprae prepared from lepromatous material actively oxidized 3,4-dihydroxyphenylalanine (DOPA) to pigmented products, in vitro2. Among several strains of mycobactena tested (including M. tuberculosis, M.
openaire   +2 more sources

Phenoloxidases of Aspergillus nidulans

Transactions of the British Mycological Society, 1974
A maximum of five phenoloxidase bands of the tyrosinase type were detected on Polyacrylamide gels after disk electrophoresis of extracts of Aspergillus nidulans . One band could be distinguished from the others because it was thermolabile. At least one band was present in all samples analysed.
S.D. Martinelli, B.W. Bainbridge
openaire   +1 more source

Properties of Phenoloxidase in Mycobacterium leprae

Nature, 1968
Mycobacterium leprae separated from infected human tissues, oxidizes 3,4-dihydroxyphenylalanine (dopa) and a few other phenolic compounds1–3. This property is not shown by several other mycobacteria1,2,4. Phenoloxidase occurs in vertebrate melanocytes and is widely distributed in the plant kingdom5. (E.C.
openaire   +2 more sources

Multiple Forms of Phenoloxidase

Journal of Food Science, 1967
SUMMARY— The phenoloxidase system in the tissues of mushrooms, potatoes, and apples was investigated using a polyacrylamide electrophoretic technique. The enzyme system was shown to exhibit the phenomenon of multiple forms.
S. M. CONSTANTINIDES, C. L. BEDFORD
openaire   +1 more source

Processing of crayfish hemocyanin subunits into phenoloxidase

Biochemical and Biophysical Research Communications, 2004
Hemocyanin and phenoloxidase are both copper-binding proteins involved in the immune system for a wide range of animal species. In crayfish, these proteins were purified and characterized from plasma and hemocytes, respectively. Recently, we have reported that the processing of one of the hemocyanin subunits occurs by a proteolytic cleavage under ...
So Young, Lee, Bok Luel, Lee, Kenneth, Söderhäll   +2 more
openaire   +2 more sources

Molecular Forms of Apple Phenoloxidase

Biochemie und Physiologie der Pflanzen, 1979
Summary Qualitative differences of dimeric (mol. wt. 60,000—70,000 D) and monomeric (mol. wt. 30,000 to 40,000 D) forms of phenoloxidase (EC 1.10.3.1) isolated from the fruits of two winter apple varieties were studied. It is shown that both forms differ distinctly by k m values and Hill coefficients.
M.N. Zaprometov, G.B. Samorodova-Bianki, S.A. Strel'tsina   +2 more
openaire   +1 more source