Results 171 to 180 of about 3,448 (206)

Production of phosphatidylethanol by phospholipase D phosphatidyl transferase in intact or dispersed pancreatic islets: Evidence for the in situ metabolism of phosphatidylethanol

Archives of Biochemistry and Biophysics, 1990
To determine if phospholipase D is present in intact adult islets, we took advantage of the fact that, in the presence of ethanol, this enzyme generates phosphatidylethanol via transphosphatidylation. Extracts of cells prelabeled with [14C]arachidonate, [14C]myristate, or [14C]stearate were analyzed via three TLC systems; the identify of ...
S A, Metz, M, Dunlop
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Formation of phosphatidylethanol from endogenous phosphatidylcholines in animal tissues from pig, calf, and goat [PDF]

Forensic Science International, 2018
In the presence of alcohol, phosphatidylcholine (PC) is transformed to the direct alcohol biomarker phosphatidylethanol (PEth). This reaction is catalyzed by the enzyme phospholipase D (PLD) and dependent on substrate availability. As recent methods have
Stefan Schuerch, Wolfgang Weinmann, Marc Luginbuehl   +2 more
exaly   +4 more sources

Analysis of the alcohol biomarker phosphatidylethanol by NACE with on‐line ESI‐MS

ELECTROPHORESIS, 2010
AbstractPhosphatidylethanol (Peth), a group of aberrant phospholipids formed in cell membranes in the presence of ethanol, has been recently proposed as biomarker of chronic alcohol abuse. The aim of this study was to develop a new analytical method, based on NACE online coupled with a mass spectrometer for the analysis of Peth in blood.
Nalesso A, Viel G, Cecchetto G, Frison G, Ferrara SD   +4 more
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Phosphatidylethanol formation in rat organs after ethanol treatment

Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1984
An abnormal acidic phospholipid was found in high concentration in kidney and brain, and also in other organs of rats exposed to ethanol by i.p. injection or by a liquid diet. The compound could be identified as phosphatidylethanol. Phosphatidylethanol is probably formed in cell membranes by a phospholipase D-catalyzed transphosphatidylation reaction.
C, Alling, L, Gustavsson, J E, Månsson, G, Benthin, E, Anggård   +4 more
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[Formation of phosphatidylethanol in alcoholic intoxication].

Voprosy meditsinskoi khimii, 1988
Phospholipid composition was studied in membranes of various tissues as well as of mitochondria and erythrocytes of rats with alcohol intoxication. A new phospholipid--phosphatidyl ethanol, which was absent in tissues of control animals, was detected in liver, heart, kidney, small intestine tissues as well as in erythrocytes and liver mitochondria of ...
M M, Rakhimov, K T, Almatov, D T, Mirtalipov, G M, Kasimova, N I, Khodzhaeva   +4 more
openaire   +1 more source

Inter Individual Variation and Factors Regulating the Formation of Phosphatidylethanol

Alcoholism: Clinical and Experimental Research, 2019
BackgroundAlcohol use disorders are a major but often unrecognized health problem. Alcohol markers can therefore be of great value for diagnosis, follow‐up, and treatment evaluation. Phosphatidylethanol in blood (B‐PEth) is an alcohol biomarker with higher clinical sensitivity and specificity than commonly used alcohol markers but has shown a ...
Joanna Stenton, Lisa Walther, Therese Hansson, Anders Andersson, Anders Isaksson   +4 more
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Phosphatidylethanol; clinical significance and biochemical basis

2001
As shown in figure 1, ethanol can be metabolized by oxidative and nonoxidative pathways (Lieber, 1995). In the oxidative pathway, ethanol is converted to acetal-dehyde through the action of alcohol dehydrogenases, the microsomal ethanol-oxidizing system, or catalase.
Arthur Varga, Kristian Moller, Per Hansson, Steina Aradottir, Christer Alling   +4 more
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Influence of Ethanol and Phosphatidylethanol on the Activity of Pancreatic Phospholipase A2

Biochemistry (Moscow), 2001
Hydrolysis of dioleoylphosphatidylethanol (DOPEt) and dioleoylphosphatidylcholine (DOPC) catalyzed by phospholipase A2 (PLA2) from porcine pancreas has been studied in single-component and binary liposomes in the absence and in the presence of ethanol. DOPEt (an anionic phospholipid) was found to increase the rate of hydrolysis of zwitterionic DOPC in ...
M A, Kisel, S V, Kuchuro, N M, Litvinko
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Improving screening for alcohol consumption during pregnancy with phosphatidylethanol

Reproductive Toxicology, 2017
The study objective was to compare rates of alcohol use between urine ethanol testing and self- reporting (Method: 1) and Phosphatidylethanol (PEth) dried blood spot testing and self-reporting (Method: 2).This was a prospective observational study in an obstetric clinic with universal alcohol screening.Method: 1 identified 11 patients with alcohol use (
Luis A, Bracero, Stefan, Maxwell, Afua, Nyanin, Dara J, Seybold, April, White, Mike, Broce   +5 more
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Use of Phosphatidylethanol Testing in Patients With Liver Disease

American Journal of Gastroenterology, 2023
Sahil, Khanna, Neeral L, Shah, Curtis K, Argo   +2 more
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