Exploring complex cellular phenotypes and model-guided strain design with a novel genome-scale metabolic model of Clostridium thermocellum DSM 1313 implementing an adjustable cellulosome [PDF]
Additional file 1.
Cong T. Trinh+4 more
core +7 more sources
Exploring biochemical and functional features of Leishmania major phosphoenolpyruvate carboxykinase [PDF]
This work reports the first functional characterization of leishmanial PEPCK. The recombinant Leishmania major enzyme (Lmj-PEPCK) exhibits equivalent kcat values for the phosphoenolpyruvate (PEP) and oxaloacetate (OAA) forming reactions.
Giordana, Lucila+2 more
core +1 more source
ABSTRACT Plasmodiophora brassicae is one of the most devastating threats to Brassicaceae crops. However, the molecular mechanisms underlying clubroot disease remain unclear. Initial proteomics results led us to hypothesize that HSP70 proteins regulate host–P. brassicae interactions by modulating both plant defenses and pathogen activity.
Romana Kopecká+7 more
wiley +1 more source
Aluminum Fluorides as Noncovalent Lewis Acids in Proteins: The Case of Phosphoryl Transfer Enzymes
The abundance, directionality and energetic features of O ⋅ ⋅ ⋅ Al Triel Bonds in biological systems have been studied herein combining a search in the PDB (Protein Data Bank) with Quantum Mechanics calculations at the PBE0‐D3/def2‐TZVPD level of theory. Results indicate the noncovalent and attractive nature of the interaction, being important to fully
Sergi Burguera+2 more
wiley +1 more source
Camellia seed oil is rich in bioactive compounds, which could promote the in vitro proliferation of Akkermansia muciniphila by reducing the oxidation–reduction potential of the media and activating the metabolism pathways involving energy and nucleic acid metabolism, protein synthesis, and environmental adaptation, thereby facilitating the nutrient ...
Xi Chen+3 more
wiley +1 more source
Biochemical characterization of phosphoenolpyruvate carboxykinases from Arabidopsis thaliana [PDF]
ATP-dependent phosphoenolpyruvate carboxykinases (PEPCKs, EC 4.1.1.49) from C4 and CAM plants have been widely studied due to their crucial role in photosynthetic CO2 fixation.
Figueroa, Carlos Maria+5 more
core +1 more source
ABSTRACT Background: Human macrophages generate antimicrobial reactive nitrogen species in response to infection by Mycobacterium tuberculosis (Mtb). Exposure to these redox‐reactive compounds induces stress response in Mtb, which can affect posttranslational modifications (PTM). Methods: Here, we present the global analysis of the PTM acylation of Mtb
Alemayehu Godana Birhanu+3 more
wiley +1 more source
The mitochondrial and cytosolic forms of avian phosphoenolpyruvate carboxykinase (GTP) are encoded by different messenger RNAs. [PDF]
Previous work from our laboratory (Watford, M., Hod, Y., Chiao, Y. B., Utter M. F., and Hanson R. W. (1981) J. Biol. Chem. 256, 10023-10027) indicated that in the chicken, hepatic phosphoenolpyruvate carboxykinase is in the mitochondria, whereas kidney contains both a mitochondrial and cytosolic form of the enzyme. In the present study the two forms of
Merton F. Utter+2 more
openaire +2 more sources
Proteomic profiling reveals the transglutaminase-2 externalization pathway in kidneys after unilateral ureteric obstruction [PDF]
Increased export of transglutaminase-2 (TG2) by tubular epithelial cells (TECs) into the surrounding interstitium modifies the extracellular homeostatic balance, leading to fibrotic membrane expansion.
A.+22 more
core +1 more source
Abstract The coordination of assimilation pathways for all the elements that make up cellular components is a vital task for every organism. Integrating the assimilation and use of carbon (C) and nitrogen (N) is of particular importance because of the high cellular abundance of these elements.
Lisa Scholtysek+3 more
wiley +1 more source