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Evolution of Phosphofructokinase [PDF]
, 1988 Abstract : Comparative sequence data suggests that mammalian phosphofructokinase (PFK) has evolved from a procaryotic precursors by gene duplication, fusion, and mutation of previous catalytic sites into new regulatory ligand binding sites. We are examining this process by two approaches.
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Interaction of phosphofructokinase with antibodies
Biochimica et Biophysica Acta (BBA) - Enzymology, 1975The allosteric properties of phosphofructokinase (EC 2.7.1.11) from rabbit muscle are influenced by enzyme concentration, most probably due to changes in the association state of the enzyme. In this study, the behaviour of dispersed pre-cipitates of phosphofructolinase as produced by treatment with antibodies has been investigated.
Margaret Bartholomé-Dönnicke +1 more
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Archives of Biochemistry and Biophysics, 1979
A crosslinked hexamer of Phosphofructokinase from Dunaliella salina has been prepared and purified by ammonium sulfate fractionation, DEAE-cellulose chromatography, and gel chromatography on BioGel-A 5 m. The crosslinked enzyme is characterized by sedimentation coefficient so20,w = 14.7 S, translational diffusion coefficient D20,w = 2.88 × 10−7 cm2/s ...
H.Hasko Paradies +2 more
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A crosslinked hexamer of Phosphofructokinase from Dunaliella salina has been prepared and purified by ammonium sulfate fractionation, DEAE-cellulose chromatography, and gel chromatography on BioGel-A 5 m. The crosslinked enzyme is characterized by sedimentation coefficient so20,w = 14.7 S, translational diffusion coefficient D20,w = 2.88 × 10−7 cm2/s ...
H.Hasko Paradies +2 more
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Clinica Chimica Acta, 1975
A case of chronic non-spherocytic hemolytic anemia due to partial erythrocyte phosphofructokinase deficiency (61% of normal) is reported. Immunological studies in hemolystates, using anti-muscle and anti-leukocyte phosphofructokinase antisera, seemed to indicate that an isozyme of the muscle type was deficient in the patient.
Pierre Bovin +3 more
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A case of chronic non-spherocytic hemolytic anemia due to partial erythrocyte phosphofructokinase deficiency (61% of normal) is reported. Immunological studies in hemolystates, using anti-muscle and anti-leukocyte phosphofructokinase antisera, seemed to indicate that an isozyme of the muscle type was deficient in the patient.
Pierre Bovin +3 more
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International Journal of Biochemistry, 1973
Abstract 1. 1. An isolation method for human leucocyte phosphofructokinase is described. 2. 2. The enzyme resembles the crythrocyte phosphofructokinase with respect to the kinetics, the electrophoretic behaviour, the influence of several ligands, and the various molecular weights. 3. 3.
P. Van Dijck +3 more
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Abstract 1. 1. An isolation method for human leucocyte phosphofructokinase is described. 2. 2. The enzyme resembles the crythrocyte phosphofructokinase with respect to the kinetics, the electrophoretic behaviour, the influence of several ligands, and the various molecular weights. 3. 3.
P. Van Dijck +3 more
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Pasteur Effect and Phosphofructokinase
1974Publisher Summary The Pasteur effect can be defined in two parts: (1) as the action of oxygen in diminishing the carbohydrate uptake and its catabolism; (2) as the action of oxygen in suppressing or decreasing the accumulation of the products of anaerobic metabolism.
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Studies on the structure of yeast phosphofructokinase
Biochimie, 1984In this paper, we describe an efficient procedure for the purification of yeast phosphofructokinase. This procedure eliminates any time delay and enables to obtain an enzyme with minimum proteolytic alterations. The molecular weights of the oligomeric enzyme and of its constitutive subunits were both evaluated by means of several independent methods ...
Jeannine M. Yon +7 more
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[10] Phosphofructokinase from yeast
1982Publisher Summary This chapter discusses the purification of enzyme phosphofructokinase from yeast and describes some properties of purified enzyme. Phosphofructokinase is an allosteric enzyme that catalyzes the transfer of the γ-phosphoryl group of MgATP to carbon atom one of D(-)-fructofuranose 6-phosphate and produces D(-)-fructofuranose 1,6 ...
Kopperschläger G, Hofmann E
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Rat liver phosphofructokinase isozymes
Archives of Biochemistry and Biophysics, 1974Abstract The labile phosphofructokinase activity of rat liver was found to be stabilized and efficiently extracted in 50 m m Tris-HCl, pH 8.0, 50 m m NaF, 10 m m dithiothreitol, and 1.0 m m ATP. By the method of DEAE-cellulose chromatography liver phosphofructokinase activity could be resolved into two isozymes. The major isozyme which was 85% of
George Weber, George A. Dunaway
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Purification of the phosphofructokinase regulatory factors
Archives of Biochemistry and Biophysics, 1984In this paper, the existence and purification of two species of phosphofructokinase regulatory factor activity are reported. The purification procedure included liver homogenization and ultracentrifugation, a 93 degrees C heat step on the supernate, precipitation with ammonium sulfate, DEAE-cellulose column chromatography, and Sephadex G-75 (fine ...
Dale A. Kruep, George Dunaway
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