Results 221 to 230 of about 108,655 (245)
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2010
Publisher Summary Phospholipase D (PLD) is associated with hydrolysis of the phosphodiester bond of the glycerolipid phosphatidylcholine (PC) to form phosphatidic acid (PA) and choline. PLD superfamily catalyzes a transphosphatidylation reaction that, depending on the specific gene product, can employ a wide variety of primary alcohol-like ...
Wenjuan Su, Michael A. Frohman
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Publisher Summary Phospholipase D (PLD) is associated with hydrolysis of the phosphodiester bond of the glycerolipid phosphatidylcholine (PC) to form phosphatidic acid (PA) and choline. PLD superfamily catalyzes a transphosphatidylation reaction that, depending on the specific gene product, can employ a wide variety of primary alcohol-like ...
Wenjuan Su, Michael A. Frohman
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Phospholipase D structure and regulation
Chemistry and Physics of Lipids, 1999The recent identification of cDNA clones for phospholipase D has opened the door to new types of investigations into its structure and regulation. PLD activity has been found to be encoded by at least two different genes that contain catalytic domains that relate their mechanism of action to phosphodiesterases. In vivo roles for PLD suggest that it may
Andrew J. Morris, Michael A. Frohman
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Dimerization of Phospholipase D Isozymes
Biochemical and Biophysical Research Communications, 2002Two mammalian phospholipase D (PLD) isozymes (PLD1 and PLD2) have been reported. In this study, we differentially tagged these isozymes with enhanced green fluorescent protein (EGFP-rPLD1 and EGFP-rPLD2) or Xpress peptide epitope (Xpress-rPLD1 and Xpress-rPLD2) to examine the association between these isozymes.
Yoonseok Kam, John H. Exton
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2009
Phospholipase D (PLD) is involved in different plant processes, ranging from responses to abiotic and biotic stresses to plant development and seed quality. The PLD family consists of multiple members that have distinguishable biochemical and regulatory properties.
Wenhua Zhang +4 more
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Phospholipase D (PLD) is involved in different plant processes, ranging from responses to abiotic and biotic stresses to plant development and seed quality. The PLD family consists of multiple members that have distinguishable biochemical and regulatory properties.
Wenhua Zhang +4 more
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1993
Publisher Summary This chapter discusses the detection and characterization of the soluble phospholipase D. Phospholipase D is an important component in cellular signal transduction mechanisms. A unique property of phospholipase D is its ability to catalyze a transphosphatidylation reaction by which the phosphatidyl moiety of the phospholipid ...
M. Motasim Billah +2 more
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Publisher Summary This chapter discusses the detection and characterization of the soluble phospholipase D. Phospholipase D is an important component in cellular signal transduction mechanisms. A unique property of phospholipase D is its ability to catalyze a transphosphatidylation reaction by which the phosphatidyl moiety of the phospholipid ...
M. Motasim Billah +2 more
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Isolation of an Activator for Phospholipase D
Nature, 1959WHILE working on the purification of phospholipase D for use in connexion with other studies on phos-pholipids1 we observed that incubation with the enzyme released choline from commercial soy bean lecithin, but not from synthetic L-α-dipalmitoyl lecithin or carefully purified lecithins from egg, yeast and soy beans.
Herbert Weiss +2 more
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Measurement of Phospholipase D Activity
Analytical Biochemistry, 1997Phosphodiesteric cleavage of phosphatidylcholine by members of a growing family of phospholipases D produces choline and phosphatidic acid. These enzymes can also catalyse a transphosphatidylation reaction in which the aliphatic chain of a primary alcohol is transferred to the phosphatidyl moiety of the phosphatidic acid product.
JoAnne Engebrecht +2 more
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Phospholipase D and cell signaling
Current Opinion in Immunology, 1993Phospholipase D, which hydrolyzes phospholipids (primarily phosphatidylcholine) to generate phosphatidic acid, has emerged as a critical component in cellular signal transduction. Research during the past year has confirmed and extended the view that phosphatidic acid and its dephosphorylated product, sn-1,2-diacylglycerol, are important intracellular ...
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Biochemical Analysis of Phospholipase D
2007Phospholipase D (PLD) is distributed widely in nature, being present in various isoforms in bacteria, protozoa, fungi, plants, and animals. It catalyzes the hydrolysis of phospholipids, primarily phosphatidylcholine (PC), into phosphatidic acid (PA) and the head group, choline.
Anita M. Preininger +4 more
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A spectrophotometric assay for phospholipase D
Analytica Chimica Acta, 1995Abstract The preparation of phosphatidyl- p -nitrophenol and its use as a substrate in the non-enzyme based assay for the quantitative evaluation of phospholipase D activity is described. The solution of the substrate in Tris buffer pH 8 and Triton X-100 is mixed directly with an aliquot of a fermentation broth or a plant extract and the absorbance ...
D'ARRIGO, PAOLA +3 more
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