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Phosphoproteome Analysis in Yeast
2003Publisher Summary This chapter describes yeast phosphoproteome analysis using the specific example of the effects of the Reg1p regulatory subunit of Glc7p (PP-1C) on the yeast phosphoproteome. The chapter presents genomics and proteomics linked in order to determine the direct effects of a given gene product.
Rupa, Ray, Timothy A, Haystead
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SILAC-Based Temporal Phosphoproteomics
2014In recent years, thanks to advances in Mass Spectrometry (MS)-based quantitative proteomics, studies on signaling pathways have moved from a detailed description of individual components to system-wide analysis of entire signaling cascades, also providing spatio-temporal views of intracellular pathways.
Francavilla, Chiara +3 more
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Enrichment Strategies in Phosphoproteomics
2016The comprehensive study of the phosphoproteome is heavily dependent on appropriate enrichment strategies that are most often, but not exclusively, carried out on the peptide level. In this chapter, I give an overview of the most widely used techniques. In addition to dedicated antibodies, phosphopeptides are enriched by their selective interaction with
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Deciphering the human phosphoproteome
Nature Biotechnology, 2020Giulia Franciosa +2 more
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