Biocompatible Ir(III) Complexes as Oxygen Sensors for Phosphorescence Lifetime Imaging [PDF]
Molecules, 2021 Synthesis of biocompatible near infrared phosphorescent complexes and their application in bioimaging as triplet oxygen sensors in live systems are still challenging areas of organometallic chemistry.
Ilya S. Kritchenkov +14 more
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High-Resolution Phosphorescence Lifetime Imaging (PLIM) of Bones [PDF]
Applied Sciences, 2022 For the first time, the time-resolved two-photon excited autophosphorescence of non-labeled biological specimens was investigated by phosphoresce lifetime imaging with microscopic spatial resolution.
Hans Georg Breunig, Karsten König
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Quantitation of cerebral oxygen tension using phasor analysis and phosphorescence lifetime imaging microscopy (PLIM) [PDF]
Biomedical Optics Express, 2021 Time-domain measurements for fluorescence lifetime imaging microscopy (FLIM) and phosphorescence lifetime imaging microscopy (PLIM) are conventionally computed by nonlinear curve fitting techniques to model the time-resolved profiles as mono- or multi-exponential decays.
Sava Sakadžić +2 more
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Effects of Photodynamic Therapy on Tumor Metabolism and Oxygenation Revealed by Fluorescence and Phosphorescence Lifetime Imaging [PDF]
International Journal of Molecular SciencesThis work was aimed at the complex analysis of the metabolic and oxygen statuses of tumors in vivo after photodynamic therapy (PDT). Studies were conducted on mouse tumor model using two types of photosensitizers—chlorin e6-based drug Photoditazine predominantly targeted to the vasculature and genetically encoded photosensitizer KillerRed targeted to ...
Varvara Dudenkova +2 more
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Determination of the physiological range of oxygen tension in bone marrow monocytes using two-photon phosphorescence lifetime imaging microscopy [PDF]
Scientific Reports, 2022 Oxygen is a key regulator of both development and homeostasis. To study the role of oxygen, a variety of in vitro and ex vivo cell and tissue models have been used in biomedical research. However, because of ambiguity surrounding the level of oxygen that
Ayako Narazaki +8 more
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Fluorescence and phosphorescence lifetime imaging reveals a significant cell nuclear viscosity and refractive index changes upon DNA damage [PDF]
Scientific Reports, 2023 Cytoplasmic viscosity is a crucial parameter in determining rates of diffusion-limited reactions. Changes in viscosity are associated with several diseases, whilst nuclear viscosity determines gene integrity, regulation and expression.
Ellen Clancy +7 more
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Line scanning mechanical streak camera for phosphorescence lifetime imaging. [PDF]
Opt Express, 2020 Phosphorescence lifetime measurement holds great importance in life sciences and material sciences. Due to the long lifetime of phosphorescence emission, conventional approaches based on point scanning time-domain recording suffer from long recording time and low signal-to-noise ratio (SNR).
Wang C, Cheng Z, Gan W, Cui M.
europepmc +3 more sources
Analysis of tumour oxygenation in model animals on a phosphorescence lifetime based macro-imager
Scientific Reports, 2023 Monitoring of tissue O2 is essential for cancer development and treatment, as hypoxic tumour regions develop resistance to radio- and chemotherapy. We describe a minimally invasive technique for the monitoring of tissue oxygenation in developing grafted ...
Alexander V. Zhdanov +5 more
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Tomographic imaging of oxygen by phosphorescence lifetime [PDF]
Applied Optics, 2006 Imaging of oxygen in tissue in three dimensions can be accomplished by using the phosphorescence quenching method in combination with diffuse optical tomography. We experimentally demonstrate the feasibility of tomographic imaging of oxygen by phosphorescence lifetime.
David F Wilson, Sergei Vinogradov
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Simultaneous Fluorescence and Phosphorescence Lifetime Imaging Microscopy in Living Cells. [PDF]
Sci Rep, 2015 AbstractIn living cells, there are always a plethora of processes taking place at the same time. Their precise regulation is the basis of cellular functions, since small failures can lead to severe dysfunctions. For a comprehensive understanding of intracellular homeostasis, simultaneous multiparameter detection is a versatile tool for revealing the ...
Jahn K, Buschmann V, Hille C.
europepmc +5 more sources