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Interactive Database for 2-Photon-Based Oxygen Phosphorescence Lifetime Imaging Data

Biophotonics Congress 2021, 2021
We present a graphical user interface-based database software for visualization, analysis, and export of experimental data of two-photon-based phosphorescence lifetime imaging of oxygen concentrations in the somatosensory cortex of awake mice.
Anna Devor, Payam Saisan
exaly   +2 more sources

Oxygen Assessment in Tumors In Vivo Using Phosphorescence Lifetime Imaging Microscopy

Methods in Molecular Biology
The oxygen level in a tumor is a crucial factor for its development and response to therapies. Phosphorescence lifetime imaging (PLIM) with the use of phosphorescent oxygen probes is a highly sensitive, noninvasive optical technique for the assessment of molecular oxygen in living cells and tissues.
Marina Shirmanova   +2 more
exaly   +3 more sources

Tomographic Imaging of Oxygen in Tissue by Phosphorescence Lifetime: A Computational Study

Biomedical Optics, 2006
In this paper using numerical simulations we demonstrate feasibility of near infrared oxygen imaging in tissue by phosphorescence lifetime in time-domain and show reconstructions of hypoxic phantoms in cylindrical model.
David F Wilson, Sergei Vinogradov
exaly   +2 more sources

Dual-Channel Phosphorescence Ratiometry and Phosphorescence Lifetime Imaging of Mitochondria-Specific Methionine Sulfoxide Reductase Activity

Journal of the American Chemical Society
Methionine sulfoxide reductases (Msrs) are essential for preserving redox homeostasis in the nervous system, with dysregulation implicated in Alzheimer's disease (AD). Conventional fluorescence-based assays for Msrs activity sensing are hampered by background interference, limited sensitivity, and inadequate quantification. This work introduces a novel
Yang Tian, Qi-Wei Zhang
exaly   +3 more sources

Phosphorescence Lifetime Imaging (PLIM) with a multimodal multiphoton tomograph

Multiphoton Microscopy in the Biomedical Sciences XXII, 2022
Hans Georg Breunig
exaly   +2 more sources

Phosphorescence lifetime imaging in turbid media

2021
In this dissertation we explore the use of highly scattered photons in an analog of conventional emission tomography. Through this technique we quantitatively reconstruct images of the spatial distribution of oxygen in turbid media. We begin with the mathematical description of diffusive light propagation in highly scattering systems and solve the ...
Vadim Soloviev, Anthony Paul Curatola
openaire   +1 more source

Singlet Oxygen Phosphorescence Lifetime Imaging Based on a Fluorescence Lifetime Imaging Microscope

The Journal of Physical Chemistry A, 2015
The feasibility of singlet oxygen phosphorescence (SOP) lifetime imaging microscope was studied on a modified fluorescence lifetime imaging microscope (FLIM). SOP results from the infrared radiative transition of O2(a(1)Δg → X(3)Σg(-)) and O2(a(1)Δg) was produced in a C60 powder sample via photosensitization process. To capture the very weak SOP signal,
Wenming, Tian   +9 more
openaire   +2 more sources

Combined fluorescence and phosphorescence lifetime imaging

Applied Physics Letters, 2016
We present a lifetime imaging technique that simultaneously records the fluorescence and phosphorescence lifetime images in confocal laser scanning systems. It is based on modulating a high-frequency pulsed laser synchronously with the pixel clock of the scanner, and recording the fluorescence and phosphorescence signals by multidimensional time ...
V. I. Shcheslavskiy   +4 more
openaire   +1 more source

Simultaneous fluorescence and phosphorescence lifetime imaging

SPIE Proceedings, 2011
We present a lifetime imaging technique that simultaneously records fluorescence and phosphorescence lifetime images in laser scanning systems. It is based on modulating a high-frequency pulsed laser by a signal synchronous with the pixel clock of the scanner, and recording the fluorescence and phosphorescence signals by multi-dimensional TCSPC ...
Wolfgang Becker   +4 more
openaire   +1 more source

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