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Luminescence lifetime imaging of ultra-long room temperature phosphorescence on a smartphone

Analytical and Bioanalytical Chemistry, 2021
Luminescence lifetime imaging plays an important role in distinguishing the luminescence decay rates in time-resolved luminescence imaging. However, traditional imaging instruments used for detecting lifetimes within milliseconds would be time-consuming when imaging ultra-long luminescence lifetimes over subseconds. Herein, we present an accessible and
Zece, Zhu   +4 more
openaire   +2 more sources

Phosphorescence lifetime imaging in turbid media: the forward problem

Applied Optics, 2003
An adequate description of the propagation of near-infrared light in highly scattering media is important for imaging hidden luminescent inhomogeneities in tissues. We present an improved approach to modeling of the forward problem based on a telegraph equation. The asymptotic solutions of the telegraph equation are derived and analyzed. The expression
Vadim, Soloviev   +2 more
openaire   +2 more sources

Phosphorescence lifetime imaging in turbid media: the inverse problem and experimental image reconstruction

Applied Optics, 2004
Three-dimensional phosphorescence lifetime imaging is a novel method for the mapping of oxygen concentration in biological tissues. We present reconstruction techniques for recovering phosphorescent objects in highly scattering media based on the telegraph equation and two regularization methods, i.e., the Tikhonov-Phillips regularization and the ...
Vadim Y, Soloviev   +2 more
openaire   +2 more sources

Phosphorescence Lifetime Imaging of Labile Zn2+ in Mitochondria via a Phosphorescent Iridium(III) Complex

Inorganic Chemistry, 2018
Phosphorescence lifetime Zn2+ imaging possesses the advantage over normal fluorescence imaging in offering the more accurate temporal-spatial Zn2+ information. Herein, we report a new phosphorescent cyclometalated Ir(III) complex with a Zn2+-chelator bearing 1,10-phenanthrolin acting as ancillary ligand, Zin-IrDPA, which displays the specific Zn2 ...
Changli Zhang   +6 more
openaire   +2 more sources

Map estimation of oxygen tension in retinal vessels with phosphorescence lifetime imaging

2012 9th IEEE International Symposium on Biomedical Imaging (ISBI), 2012
In phosphorescence lifetime imaging methods oxygen tension in retinal vessels has traditionally been indirectly determined from the estimates of intermediate variables whose noise-contaminated linear combinations are observed as phosphorescence intensity images.
Isa Yildirim, Rashid Ansari
openaire   +1 more source

Estimation of oxygen tension in retinal capillaries from phosphorescence lifetime images

2009 IEEE International Symposium on Biomedical Imaging: From Nano to Macro, 2009
Investigating the effect of retinal oxygenation abnormalities in the development of common eye diseases requires accurate assessment of oxygen tension in retinal vasculatures. Estimation of oxygen tension in retinal capillaries using phosphorescence lifetime imaging is addressed in this paper.
Isa Yildirim   +4 more
openaire   +1 more source

Phosphorescence Lifetime Imaging (PLIM): State of the Art and Perspectives

2019
This chapter reviews the status and perspectives of phosphorescence lifetime imaging (PLIM), an advanced imaging strategy that relies on phosphorescence lifetime measuring as a function of some particular biological microenvironment parameters. PLIM should be regarded as a functional imaging technique, as opposite to various forms of “localization ...
Pavel S. Chelushkin, Sergey P. Tunik
openaire   +1 more source

Phosphorescence Lifetime Imaging for Monitoring an Insertable Hydrogel Multianalyte Sensor

2024 46th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC)
Oxidoreductase enzymes paired with oxygen-sensitive phosphorescent dyes can be immobilized in sensor structures that allow for continuous monitoring following insertion with minimal insult to the tissue. However, oxidoreductase-based sensors are limited by their consumption of oxygen, requiring knowledge and control of local oxygen conditions. Further,
Brian Serivuth Ko   +2 more
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Precision and accuracy considerations for phosphorescence lifetime thermometry imaging using rapid lifetime determination

IET & ISA 60th International Instrumentation Symposium 2014, 2014
Phosphor lifetime imaging holds much promise as an accurate method for distributed temperature sensing. Several previous efforts have been directed at analyzing the potential and limitations of this technique in terms of accuracy and precision.
B. Heeg, J.I. Eldridge, T.P. Jenkins
openaire   +1 more source

Intravital phosphorescence lifetime imaging of the renal cortex accurately measures renal hypoxia

Kidney International, 2018
Renal tubulointerstitial hypoxia is recognized as a final common pathway of chronic kidney disease and is considered a promising drug target. However, hypoxia in the tubules is not well examined because of limited detection methods. Here, we devised a method to visualize renal tubular oxygen tension with spatial resolution at a cellular level using the
Yosuke, Hirakawa   +9 more
openaire   +2 more sources

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