Can Crystal Symmetry and Packing Influence the Active Site Conformation of Homohexameric Purine Nucleoside Phosphorylases? [PDF]
Croatica Chemica Acta, 2016 It is generaly believed that enzymes retain most of their functionality in the crystal form due to the large solvent content of protein crystals. This is facilitated by the fact that their natural environment in solution is not too far from the one found
Marija Luić, Zoran Štefanić
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In vitro and in vivo exploration of the cellobiose and cellodextrin phosphorylases panel in Ruminiclostridium cellulolyticum: implication for cellulose catabolism [PDF]
Biotechnology for Biofuels, 2019 Background In anaerobic cellulolytic micro-organisms, cellulolysis results in the action of several cellulases gathered in extracellular multi-enzyme complexes called cellulosomes.
Nian Liu +7 more
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Dual-Activity Mannosyltransferase Phosphorylases in Family 130 of Glycoside Hydrolases. [PDF]
ACS CatalInternational audienceWe discovered that certain mannoside-active enzymes from family GH130 possess both glycoside phosphorylase (GP) and weak glycosyltransferase (GT) activities. This dual activity was observed only in glycoside phosphorylases acting on
Ladeveze S +11 more
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Nucleoside Phosphorylases Make N7-Xanthosine, the “Non-native” Regioisomer of Xanthosine [PDF]
, 2023 Modern, highly evolved, nucleoside-processing enzymes are known to exhibit perfect regioselectivity over the glycosylation of purine nucleobases at N9. We herein report an exception to this paradigm.
Anke, Kurreck +9 more
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SOME RECENT FINDINGS IN THE BIOTECHNOLOGY OF BIOLOGICALLY IMPORTANT NUCLEOSIDES [PDF]
Biotechnologia Acta, 2013 Some recent findings in the biotechnology of biologically important nucleosides will be discussed, viz., (i) a new strategy of the cascade one-pot transformation of D-pentoses into nucleosides based on the extension and deepening of the knowledge of the ...
A. Mikhailopulo, A. I. Miroshnikov
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Disaccharide phosphorylases: Structure, catalytic mechanisms and directed evolution
Synthetic and Systems Biotechnology, 2021 Disaccharide phosphorylases (DSPs) are carbohydrate-active enzymes with outstanding potential for the biocatalytic conversion of common table sugar into products with attractive properties. They are modular enzymes that form active homo-oligomers. From a
Shangshang Sun, Chun You
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An exploration of phosphorylases for the synthesis of carbohydrate polymers [PDF]
, 2013 Phosphorylases are interesting enzymes with regard to both their role in metabolism and their use in the in vitro synthesis of carbohydrates. The disaccharide phosphorylases have attracted attention because of their strict stereo- and regiospecificity
O'Neill, Ellis
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Frontiers in Chemistry, 2023 The phosphorolysis of cello-oligosaccharides is a critical process played in the rumen by Ruminococcus albus to degrade cellulose. Cellodextrins, made up of a few glucosyl units, have gained lots of interest by their potential applications.
Alem Storani +2 more
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A C-Nucleoside Analogue of Cordycepin With High Metabolic Stability and Potent Anti-Psoriatic Activity via Microneedle Delivery. [PDF]
Adv Sci (Weinh)This work identified CPD3a as a potent, stable C‐nucleoside cordycepin derivative. When formulated into microneedle array for topical treatment, it ameliorated psoriasis by rebalancing immunity and enhancing antioxidant defenses. ABSTRACT Psoriasis is a chronic inflammatory disorder characterized by immune dysregulation and epidermal hyperplasia ...
Pan W +7 more
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Molecules, 2023 The complete mechanism behind starch regulation has not been fully characterized. However, significant progress can be achieved through proteomic approaches.
Junio Flores Castellanos +2 more
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