Results 31 to 40 of about 3,382 (177)
Beilstein Journal of Organic Chemistry, 2020 In the present work, we suggested anion exchange resins in the phosphate form as a source of phosphate, one of the substrates of the phosphorolysis of uridine, thymidine, and 1-(β-ᴅ-arabinofuranosyl)uracil (Ara-U) catalyzed by recombinant E. coli uridine
Julia N. Artsemyeva +7 more
doaj +1 more source
Recent development of phosphorylases possessing large potential for oligosaccharide synthesis
, 2013 Phosphorylases are one group of carbohydrate active enzymes involved in the cleavage and formation of glycosidic linkages together with glycoside hydrolases and sugar nucleotide-dependent glycosyltransferases.
Svensson, Birte +3 more
core +1 more source
Insights into phosphate cooperativity and influence of substrate modifications on binding and catalysis of hexameric purine nucleoside phosphorylases. [PDF]
PLoS ONE, 2012 The hexameric purine nucleoside phosphorylase from Bacillus subtilis (BsPNP233) displays great potential to produce nucleoside analogues in industry and can be exploited in the development of new anti-tumor gene therapies.
Priscila O de Giuseppe +5 more
doaj +1 more source
Découverte de nouvelles glycoside-phosphorylases pour la synthèse d'oligosaccharides
, 2020 Glycoside-phosphorylases are particular carbohydrate active enzymes (CAZymes), able to catalyze both glycoside degradation by using inorganic phosphate to breakdown osidic linkages, and synthesis by using sugar-phosphates as glycosyl donors.
Li, Ao
core +2 more sources
Diversification of 4ʹ-Methylated Nucleosides by Nucleoside Phosphorylases [PDF]
, 2021 The growing demand for 4\u27-modified nucleoside analogs in medicinal and biological chemistry is contrasted by the challenging synthetic access to these molecules and the lack of efficient diversification strategies. Herein, we report the development of
Anke, Kurreck +11 more
core +2 more sources
Microbial Cell Factories, 2022 Background Continuous processing with enzyme reuse is a well-known engineering strategy to enhance the efficiency of biocatalytic transformations for chemical synthesis.
Katharina N. Schwaiger, Bernd Nidetzky
doaj +1 more source
Heterotropic regulation and negative homotropic cooperativity
FEBS Open Bio, EarlyView.We identified a structural module common to some proteins that couple negative cooperativity with heterotropic regulation, two features that rarely coexist. These proteins are ring‐like and present an ordered asymmetry whereby noncontacting subunits are symmetric, and their tertiary structure differs from that of contacting subunits.
Veronica Morea +5 more
wiley +1 more source
A minimal cellulosome‐like system in Cellulosilyticum lentocellum
FEBS Open Bio, EarlyView.Cellulose‐degrading bacteria typically use cellulosomes, large multi‐enzyme complexes on a scaffold protein. In Cellulosilyticum lentocellum, we characterise a far smaller arrangement, a single scaffold bound to one cellulase through a single cohesin‐dockerin interaction.
John Allan +2 more
wiley +1 more source
Nucleoside phosphorylases from clostridium perfringens in the synthesis of 2',3'-dideoxyinosine
, 2010 Four Clostridium perfringens phosphorylases were subcloned, overexpressed and analyzed for their substrate specificity. DeoD(1) and PunA could use a variety of purine substrates, including an antiviral drug 2',3'-dideoxyinosine (ddI).
TERRENI, MARCO +3 more
core +2 more sources
Crystals, 2023 Uridine phosphorylases are used for biotechnological synthesis of pyrimidine derivatives and, moreover, their substrates and inhibitors are used in medicine.
Alexander A. Lashkov +3 more
doaj +1 more source