PKCɛ switches Aurora B specificity to exit the abscission checkpoint
Nature Communications, 2016 The Aurora B abscission checkpoint is activated when DNA is retained in the cleavage furrow on completion of anaphase. Here the authors show that PKCɛ directly phosphorylates Aurora B triggering a switch in Aurora B substrate specificity to elicit ...
Tanya Pike +4 more
doaj +1 more source
On the existence of Hopf bifurcations in the sequential and distributive double phosphorylation cycle [PDF]
, 2019 Protein phosphorylation cycles are important mechanisms of the post translational modification of a protein and as such an integral part of intracellular signaling and control. We consider the sequential phosphorylation and dephosphorylation of a protein
Conradi, Carsten +2 more
core +2 more sources
Tissue environment, not ontogeny, defines murine intestinal intraepithelial T lymphocytes
eLife, 2021 Tissue-resident intestinal intraepithelial T lymphocytes (T-IEL) patrol the gut and have important roles in regulating intestinal homeostasis. T-IEL include both induced T-IEL, derived from systemic antigen-experienced lymphocytes, and natural T-IEL ...
Alejandro J Brenes +8 more
doaj +1 more source
A conserved phosphorylation site regulates the transcriptional function of ETHYLENE-INSENSITIVE3-like1 in tomato [PDF]
, 2011 ETHYLENE-INSENSITIVE3/ETHYLENE-INSENSITIVE3-like (EIN3/EIL) transcription factors are important downstream components of the ethylene transduction pathway known to regulate the transcription of early ethylene-responsive genes in plants.
Abel +37 more
core +2 more sources
Nuclear CaMKII enhances histone H3 phosphorylation and remodels chromatin during cardiac hypertrophy. [PDF]
, 2013 Calcium/calmodulin-dependent protein kinase II (CaMKII) plays a central role in pathological cardiac hypertrophy, but the mechanisms by which it modulates gene activity in the nucleus to mediate hypertrophic signaling remain unclear. Here, we report that
An, Woojin +7 more
core +1 more source
PPM1H phosphatase counteracts LRRK2 signaling by selectively dephosphorylating Rab proteins
eLife, 2019 Mutations that activate LRRK2 protein kinase cause Parkinson’s disease. LRRK2 phosphorylates a subset of Rab GTPases within their Switch-II motif controlling interaction with effectors.
Kerryn Berndsen +11 more
doaj +1 more source
Journal of Advanced Research, 2023 Introduction: The structural and dynamic determinants that confer highly selective RET kinase inhibition are poorly understood. Objectives: To explore the druggability landscape of the RET active site in order to uncover structural and dynamic ...
Moustafa A. Shehata +6 more
doaj +1 more source
Translation inhibition by rocaglates is independent of eIF4E phosphorylation status [PDF]
, 2016 Rocaglates are natural products that inhibit protein synthesis in eukaryotes and exhibit antineoplastic activity. In vitro biochemical assays, affinity chromatography experiments coupled with mass spectrometry analysis, and in vivo genetic screens have ...
Cencic, Regina +4 more
core +1 more source
Determination of VPS34/PIK3C3 Activity in vitro Utilising 32P-γATP
Bio-Protocol, 2016 VPS34 is the only class III phosphatidylinositol-3-kinase (PI3K) in mammalian cells and produces the vast majority of cellular phosphatidylinositol-3-phosphate [PI(3)P].
Michael Munson, Ian Ganley
doaj +1 more source
Distinct phosphorylation clusters determines the signalling outcome of the free fatty acid receptor FFA4/GPR120 [PDF]
, 2016 It is established that long-chain free fatty acids including ω-3 fatty acids mediate an array of biological responses through members of the free fatty acid receptor family, which includes FFA4.
Alvarez-Curto, Elisa +7 more
core +2 more sources