Results 41 to 50 of about 953,432 (120)

Targeted dephosphorylation of SMAD3 as an approach to impede TGF-β signaling

iScience
Summary: TGF-β (transforming growth factor-β) signaling is involved in a myriad of cellular processes and its dysregulation has been implicated in many human diseases, including fibrosis and cancer.
Abigail Brewer, Jin-Feng Zhao, Rotimi Fasimoye, Natalia Shpiro, Thomas J. Macartney, Nicola T. Wood, Melanie Wightman, Dario R. Alessi, Gopal P. Sapkota   +8 more
doaj   +1 more source

In Vitro Reconstitution Defines the Minimal Requirements for Cdc48-Dependent Disassembly of the CMG Helicase in Budding Yeast

Cell Reports, 2019
Summary: Disassembly of the replisome is the final step of chromosome duplication in eukaryotes. In budding yeast and metazoa, cullin ubiquitin ligases are required to ubiquitylate the Cdc45-MCM-GINS (CMG) helicase that lies at the heart of the replisome,
Progya P. Mukherjee, Karim P.M. Labib
doaj   +1 more source

CMG helicase disassembly is essential and driven by two pathways in budding yeast

The EMBO Journal
The CMG helicase is the stable core of the eukaryotic replisome and is ubiquitylated and disassembled during DNA replication termination. Fungi and animals use different enzymes to ubiquitylate the Mcm7 subunit of CMG, suggesting that CMG ubiquitylation ...
Cristian Polo Rivera, Tom D Deegan, Karim P M Labib   +2 more
doaj   +1 more source

PINK1 regulated mitophagy is evident in skeletal muscles

Autophagy Reports
PINK1, mutated in familial forms of Parkinson’s disease, initiates mitophagy following mitochondrial depolarization. However, it is difficult to monitor this pathway physiologically in mice as loss of PINK1 does not alter basal mitophagy levels in most ...
Francois Singh, Lea Wilhelm, Alan R. Prescott, Kevin Ostacolo, Jin-Feng Zhao, Margret H. Ogmundsdottir, Ian G. Ganley   +6 more
doaj   +1 more source

Neurymenolide A, a Novel Mitotic Spindle Poison from the New Caledonian Rhodophyta Phacelocarpus neurymenioides

Marine Drugs, 2019
The marine α-pyrone macrolide neurymenolide A was previously isolated from the Fijian red macroalga, Neurymenia fraxinifolia, and characterized as an antibacterial agent against antibiotic-resistant strains that also exhibited moderate cytotoxicity ...
Sofia-Eléna Motuhi, Omid Feizbakhsh, Béatrice Foll-Josselin, Blandine Baratte, Claire Delehouzé, Arnaud Cousseau, Xavier Fant, Jeannette Chloë Bulinski, Claude Elisabeth Payri, Sandrine Ruchaud, Mohamed Mehiri, Stéphane Bach   +11 more
doaj   +1 more source

Targeted dephosphorylation of TFEB promotes its nuclear translocation

iScience
Summary: Reversible phosphorylation of the transcription factor EB (TFEB) coordinates cellular responses to metabolic and other stresses. During nutrient replete and stressor-free conditions, phosphorylated TFEB is primarily localized to the cytoplasm ...
Jin-Feng Zhao, Natalia Shpiro, Gajanan Sathe, Abigail Brewer, Thomas J. Macartney, Nicola T. Wood, Florentina Negoita, Kei Sakamoto, Gopal P. Sapkota   +8 more
doaj   +1 more source

Characterisation of the Cullin‐3 mutation that causes a severe form of familial hypertension and hyperkalaemia

EMBO Molecular Medicine, 2015
Deletion of exon 9 from Cullin‐3 (CUL3, residues 403–459: CUL3Δ403–459) causes pseudohypoaldosteronism type IIE (PHA2E), a severe form of familial hyperkalaemia and hypertension (FHHt).
Frances‐Rose Schumacher, Keith Siew, Jinwei Zhang, Clare Johnson, Nicola Wood, Sarah E Cleary, Raya S Al Maskari, James T Ferryman, Iris Hardege, Yasmin, Nichola L Figg, Radoslav Enchev, Axel Knebel, Kevin M O'Shaughnessy, Thimo Kurz   +14 more
doaj   +1 more source

Redox takes control

eLife
A study of two enzymes in the brain reveals new insights into how redox reactions regulate the activity of protein kinases.
Iván Plaza-Menacho
doaj   +1 more source

Time-resolved mitochondrial screen identifies regulatory components of oxidative metabolism

EMBO Reports
Defects in mitochondrial oxidative metabolism underlie many genetic disorders with limited treatment options. The incomplete annotation of mitochondrial proteins highlights the need for a comprehensive gene inventory, particularly for Oxidative ...
Marcos Zamora-Dorta, Sara Laine-Menéndez, David Abia, Pilar González-García, Luis C López, Paula Fernández-Montes, Enrique Calvo, Jesús Vázquez, José Antonio Enríquez, Eduardo Balsa   +9 more
doaj   +1 more source

ATP functions as a pathogen-associated molecular pattern to activate the E3 ubiquitin ligase RNF213

Nature Communications
The giant E3 ubiquitin ligase RNF213 is a conserved component of mammalian cell-autonomous immunity, limiting the replication of bacteria, viruses and parasites.
Juraj Ahel, Arda Balci, Victoria Faas, Daniel B. Grabarczyk, Roosa Harmo, Daniel R. Squair, Jiazhen Zhang, Elisabeth Roitinger, Frederic Lamoliatte, Sunil Mathur, Luiza Deszcz, Lillie E. Bell, Anita Lehner, Thomas L. Williams, Hanna Sowar, Anton Meinhart, Nicola T. Wood, Tim Clausen, Satpal Virdee, Adam J. Fletcher   +19 more
doaj   +1 more source