Results 1 to 10 of about 1,744 (112)
CMG helicase disassembly is essential and driven by two pathways in budding yeast [PDF]
EMBO JournalThe CMG helicase is the stable core of the eukaryotic replisome and is ubiquitylated and disassembled during DNA replication termination. Fungi and animals use different enzymes to ubiquitylate the Mcm7 subunit of CMG, suggesting that CMG ubiquitylation ...
Cristian Polo Rivera +2 more
exaly +4 more sources
Cell Reports, 2017 Disassembly of the Cdc45-MCM-GINS (CMG) DNA helicase is the key regulated step during DNA replication termination in eukaryotes, involving ubiquitylation of the Mcm7 helicase subunit, leading to a disassembly process that requires the Cdc48 “segregase ...
Michael H Tatham +2 more
exaly +4 more sources
Cell Reports, 2019 Summary: Disassembly of the replisome is the final step of chromosome duplication in eukaryotes. In budding yeast and metazoa, cullin ubiquitin ligases are required to ubiquitylate the Cdc45-MCM-GINS (CMG) helicase that lies at the heart of the replisome,
Karim Labib
exaly +4 more sources
Synergism between CMG helicase and leading strand DNA polymerase at replication fork [PDF]
Nature Communications, 2023 The replisome that replicates the eukaryotic genome consists of at least three engines: the Cdc45-MCM-GINS (CMG) helicase that separates duplex DNA at the replication fork and two DNA polymerases, one on each strand, that replicate the unwound DNA. Here,
Zhichun Xu +10 more
doaj +2 more sources
Duplex DNA engagement and RPA oppositely regulate the DNA-unwinding rate of CMG helicase [PDF]
Nature Communications, 2020 During eukaryotic chromosome replication cells utilize ring-shaped CMG helicase that separates the two strands of the DNA double helix. Here the authors reveal that CMG helicase activity is inhibited by duplex DNA engagement at the fork, which is ...
Hazal B. Kose +4 more
doaj +2 more sources
CMG helicase disassembly is controlled by replication fork DNA, replisome components and a ubiquitin threshold [PDF]
eLife, 2020 The eukaryotic replisome assembles around the CMG helicase, which stably associates with DNA replication forks throughout elongation. When replication terminates, CMG is ubiquitylated on its Mcm7 subunit and disassembled by the Cdc48/p97 ATPase.
Tom D Deegan +4 more
doaj +2 more sources
Mechanistic models of asymmetric hand-over-hand translocation and nucleosome navigation by CMG helicase [PDF]
Nature CommunicationsFaithful replication of eukaryotic chromatin requires the CMG helicase to translocate directionally along single-stranded DNA (ssDNA) while unwinding double-stranded DNA (dsDNA) and navigating nucleosomes.
Fritz Nagae +4 more
doaj +2 more sources
DNA unwinding mechanism of a eukaryotic replicative CMG helicase [PDF]
Nature Communications, 2020 The DNA duplex is known to be split apart in a steric exclusion manner during replication, but the specific mechanism has remained unclear. Here the authors present a cryo-EM structure of a eukaryotic replicative CMG helicase on forked DNA, revealing the
Zuanning Yuan +5 more
doaj +2 more sources
Solving the MCM paradox by visualizing the scaffold of CMG helicase at active replisomes [PDF]
Nature Communications, 2022 For several decades the MCM2-7 proteins, the core of the DNA replicative helicase, eluded detection at DNA replication sites. Here, the authors solve this conundrum by gene editing, which enables visualization of replication dynamics in living cells.
Hana Polasek-Sedlackova +4 more
doaj +2 more sources
Molecular Basis for ATP-Hydrolysis-Driven DNA Translocation by the CMG Helicase of the Eukaryotic Replisome [PDF]
Cell Reports, 2019 Summary: In the eukaryotic replisome, DNA unwinding by the Cdc45-MCM-Go-Ichi-Ni-San (GINS) (CMG) helicase requires a hexameric ring-shaped ATPase named minichromosome maintenance (MCM), which spools single-stranded DNA through its central channel.
Patrik Eickhoff +11 more
doaj +2 more sources