Topological stress regulates replication fork dynamics in unperturbed S phase [PDF]
Nature CommunicationsAccurate genome duplication requires tight-regulation of replication fork progression, and disruptions to this process are a major source of genomic instability, yet how fork dynamics are controlled during unperturbed S-phase remains unclear.
Deepika Jayaprakash +8 more
doaj +2 more sources
FET proteins and PARylation-dependent condensates promote replication fork reversal and genome stability [PDF]
Nature CommunicationsTargeting replication-associated DNA repair mechanisms, including the control of ADP-ribosylation by PARP1/2 and PARG, is a powerful therapeutic approach for cancer. However, the mechanisms by which PARG inhibition impacts DNA replication remain unclear.
Celeste Giansanti +4 more
doaj +2 more sources
H2BK120ub and its reader RNF169 sequentially regulate replication fork remodeling and stability [PDF]
The EMBO JournalUbiquitination of the C-terminus of histone H2B (H2BK120ub) is a key histone modification with functions in a wide array of DNA-related processes, best characterized in gene transcription and repair.
Filip D Duzanic +8 more
doaj +2 more sources
Automated mapping of DNA replication fork progression in human cells with ForkML [PDF]
Nature CommunicationsCurrent approaches to mapping fork progression in the human genome suffer from drastically low throughput. Here, we introduce ForkML, a nanopore sequencing-based method automatically positioning thousands of individual fork velocities by tracking BrdU ...
Victoria Rojat +11 more
doaj +2 more sources
An additional replication origin causes cell cycle specific DNA replication fork speed [PDF]
Frontiers in MicrobiologyReplication fork speed (RFS) in Escherichia coli has long been considered constant throughout the replication and cell cycles. In wild-type cells, the circular chromosome is duplicated bidirectionally from oriC, yielding two replication forks that ...
Ole Skovgaard
doaj +2 more sources
The linker histone H1–BRCA1 axis is a crucial mediator of replication fork stability
Life Science Alliance, 2023 The replication-dependent histones H1 interact with BRCA1 upon replication stress. Cells deficient for H1 fail to recruit BRCA1 to stalled replication forks and undergo fork resection and collapse.
Meryem Ozgencil +4 more
doaj +1 more source
Gene duplication and deletion caused by over-replication at a fork barrier
Nature Communications, 2023 Replication fork stalling can provoke fork reversal to form a four-way DNA junction. This remodelling of the replication fork can facilitate repair, aid bypass of DNA lesions, and enable replication restart, but may also pose a risk of over-replication ...
Judith Oehler +2 more
doaj +1 more source
Chl1 helicase controls replication fork progression by regulating dNTP pools
Life Science Alliance, 2022 Chl1 helicase affects RPA-dependent checkpoint activation after replication fork arrest by ensuring proper dNTP levels, thereby controlling replication fork progression under stress conditions.
Amandine Batté +7 more
doaj +1 more source
Restarted replication forks are error-prone and cause CAG repeat expansions and contractions.
PLoS Genetics, 2021 Disease-associated trinucleotide repeats form secondary DNA structures that interfere with replication and repair. Replication has been implicated as a mechanism that can cause repeat expansions and contractions.
Michaela A Gold +6 more
doaj +1 more source
Telomere Replication: Solving Multiple End Replication Problems
Frontiers in Cell and Developmental Biology, 2021 Eukaryotic genomes are highly complex and divided into linear chromosomes that require end protection from unwarranted fusions, recombination, and degradation in order to maintain genomic stability.
Erin Bonnell +2 more
doaj +1 more source