Results 41 to 50 of about 508,345 (256)

JAK2V617F promotes replication fork stalling with disease-restricted impairment of the intra-S checkpoint response. [PDF]

, 2014
Cancers result from the accumulation of genetic lesions, but the cellular consequences of driver mutations remain unclear, especially during the earliest stages of malignancy.
Massie, C.E., Mullally, A, Li, J, Silber, Y, Ahn, JS, Mullally, A., Godfrey, AL, Nangalia, J., Massie, CE, Godfrey, A.L., Park, H.J., Gibbons, R.J., Silber, Y., Clynes, D, Chen, E, Nangalia, J, Ahn, J.S., Chen, E., Li, J., Gibbons, RJ, Green, AR, Park, HJ, Green, A.R., Clynes, D.   +23 more
core   +1 more source

Rif1 inhibits replication fork progression and controls DNA copy number in Drosophila

eLife, 2018
Control of DNA copy number is essential to maintain genome stability and ensure proper cell and tissue function. In Drosophila polyploid cells, the SNF2-domain-containing SUUR protein inhibits replication fork progression within specific regions of the ...
Alexander Munden, Zhan Rong, Amanda Sun, Rama Gangula, Simon Mallal, Jared T Nordman   +5 more
doaj   +1 more source

Peripheral lysosomes recruit PLEKHG3 to focal adhesions and restrain protrusion dynamics

FEBS Letters, EarlyView.
Proximity‐dependent labeling at the LAMTOR complex revealed the Rho GEF PLEKHG3 as a lysosome‐proximal protein directing the study toward the influence of lysosome positioning on actin dynamics and cell motility. We show that PLEKHG3 colocalizes with lysosomes at focal adhesion sites and observe that forced peripheral dispersion of lysosomes hinders ...
Rainer Ettelt, Ana‐Maria Sandru, Georg Vucak, Sebastian Didusch, Biljana Riemelmoser, Karin Ehrenreiter, Markus Hartl, Lukas A. Huber, Manuela Baccarini   +8 more
wiley   +1 more source

Replication factory activation can be decoupled from the replication timing program by modulating Cdk levels [PDF]

, 2010
In the metazoan replication timing program, clusters of replication origins located in different subchromosomal domains fire at different times during S phase. We have used Xenopus laevis egg extracts to drive an accelerated replication timing program in
Blow, J. Julian; id_orcid, Thomson, Alexander M., Gillespie, Peter J., Peter J. Gillespie, J. Julian Blow, Blow, J. Julian, Alexander M. Thomson   +6 more
core   +1 more source

FBH1 Catalyzes Regression of Stalled Replication Forks

Cell Reports, 2015
DNA replication fork perturbation is a major challenge to the maintenance of genome integrity. It has been suggested that processing of stalled forks might involve fork regression, in which the fork reverses and the two nascent DNA strands anneal.
Kasper Fugger, Martin Mistrik, Kai J. Neelsen, Qi Yao, Ralph Zellweger, Arne Nedergaard Kousholt, Peter Haahr, Wai Kit Chu, Jiri Bartek, Massimo Lopes, Ian D. Hickson, Claus Storgaard Sørensen   +11 more
doaj   +1 more source

FORK-seq: replication landscape of the Saccharomyces cerevisiae genome by nanopore sequencing

Genome Biology, 2020
Genome replication mapping methods profile cell populations, masking cell-to-cell heterogeneity. Here, we describe FORK-seq, a nanopore sequencing method to map replication of single DNA molecules at 200-nucleotide resolution.
Magali Hennion, Jean-Michel Arbona, Laurent Lacroix, Corinne Cruaud, Bertrand Theulot, Benoît Le Tallec, Florence Proux, Xia Wu, Elizaveta Novikova, Stefan Engelen, Arnaud Lemainque, Benjamin Audit, Olivier Hyrien   +12 more
doaj   +1 more source

Recovery of arrested replication forks by homologous recombination is error-prone. [PDF]

PLoS Genetics, 2012
Homologous recombination is a universal mechanism that allows repair of DNA and provides support for DNA replication. Homologous recombination is therefore a major pathway that suppresses non-homology-mediated genome instability.
Ismail Iraqui, Yasmina Chekkal, Nada Jmari, Violena Pietrobon, Karine Fréon, Audrey Costes, Sarah A E Lambert   +6 more
doaj   +1 more source

Interplay between chromosomal architecture and termination of DNA replication in bacteria

Frontiers in Microbiology, 2023
Faithful transmission of the genome from one generation to the next is key to life in all cellular organisms. In the majority of bacteria, the genome is comprised of a single circular chromosome that is normally replicated from a single origin, though ...
Daniel J. Goodall, Dominika Warecka, Michelle Hawkins, Christian J. Rudolph   +3 more
doaj   +1 more source

L‐aspartate oxidase provides new insights into fumarate reduction in anaerobic darkness in Synechocystis sp. PCC6803

FEBS Letters, EarlyView.
Synechocystis strains deficient in succinate dehydrogenase (SDH) secrete more succinate than the WT under dark anaerobic conditions, supporting that SDH then primarily acts as SDH, not as a fumarate reductase. L‐aspartate oxidase (Laspo) from Synechocystis is functional under anaerobic conditions, reducing fumarate to succinate.
Kateryna Kukil, Jeffrey A. Hawkes, Cecilia Blikstad, Pia Lindberg   +3 more
wiley   +1 more source

Proofreading deficiency of Pol I increases the levels of spontaneous rpoB mutations in E. coli [PDF]

, 2011
The fidelity role of DNA polymerase I in chromosomal DNA replication in E. coli was investigated using the rpoB forward target. These experiments indicated that in a strain carrying a proofreading-exonuclease-defective form of Pol I (polAexo mutant) the ...
Jonczyk, Piotr, K. Makiela-Dzbenska, P. Jonczyk, Fijalkowska, Iwona J., Schaaper, Roel, R.M. Schaaper, I.J. Fijalkowska, Makiela-Dzbenska, Karolina   +7 more
core   +2 more sources