The consequences of replicating in the wrong orientation: Bacterial chromosome duplication without an active replication origin [PDF]
, 2015 Chromosome replication is regulated in all organisms at the assembly stage of the replication machinery at specific origins. In Escherichia coli the DnaA initiator protein regulates the assembly of replication forks at oriC.
Dimude, JU +8 more
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The replication fork trap and termination of chromosome replication [PDF]
Molecular Microbiology, 2008 SummaryBacteria that have a circular chromosome with a bidirectional DNA replication origin are thought to utilize a ‘replication fork trap’ to control termination of replication. The fork trap is an arrangement of replication pause sites that ensures that the two replication forks fuse within the terminus region of the chromosome, approximately ...
Duggin, I, Wake, R, Bell, S, Hill, T
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Replication fork collapse at replication terminator sequences [PDF]
The EMBO Journal, 2002 Replication fork arrest is a source of genome re arrangements, and the recombinogenic properties of blocked forks are likely to depend on the cause of blockage. Here we study the fate of replication forks blocked at natural replication arrest sites. For this purpose, Escherichia coli replication terminator sequences Ter were placed at ectopic positions
Bidnenko, Vladimir +2 more
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The Swr1 chromatin-remodeling complex prevents genome instability induced by replication fork progression defects. [PDF]
, 2018 Genome instability is associated with tumorigenesis. Here, we identify a role for the histone Htz1, which is deposited by the Swr1 chromatin-remodeling complex (SWR-C), in preventing genome instability in the absence of the replication fork/replication ...
Branzei, Dana +5 more
core +2 more sources
Topoisomerase II Is Crucial for Fork Convergence during Vertebrate Replication Termination
Cell Reports, 2019 Summary: Termination of DNA replication occurs when two replication forks converge upon the same stretch of DNA. Resolution of topological stress by topoisomerases is crucial for fork convergence in bacteria and viruses, but it is unclear whether similar
Darren R. Heintzman +4 more
doaj +1 more source
RAD51 is a druggable target that sustains replication fork progression upon DNA replication stress.
PLoS ONE, 2022 Solving the problems that replication forks encounter when synthesizing DNA is essential to prevent genomic instability. Besides their role in DNA repair in the G2 phase, several homologous recombination proteins, specifically RAD51, have prominent roles
Sonia Feu +5 more
doaj +1 more source
Cohesin causes replicative DNA damage by trapping DNA topological stress [PDF]
, 2020 DNA topological stress inhibits DNA replication fork (RF) progression and contributes to DNA replication stress. In Saccharomyces cerevisiae, we demonstrate that centromeric DNA and the rDNA array are especially vulnerable to DNA topological stress ...
Baxter, Jonathan +2 more
core +1 more source
Checkpoints are blind to replication restart and recombination intermediates that result in gross chromosomal rearrangements [PDF]
, 2015 Replication fork inactivation can be overcome by homologous recombination, but this can cause gross chromosomal rearrangements that subsequently missegregate at mitosis, driving further chromosome instability.
A Janssen +39 more
core +1 more source
Replication fork stalling elicits chromatin compaction for the stability of stalling replication forks [PDF]
Proceedings of the National Academy of Sciences, 2019 Significance Stalling replication forks require strict cellular regulations for their stability. The intra-S phase checkpoint has been demonstrated essential for preventing stalling/stalled replication forks from collapse. In this study, a cellular pathway was uncovered that regulates the stabilization of stalling replication forks.
Gang, Feng +7 more
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Activation of mammalian Chk1 during DNA replication arrest: a role for Chk1 in the intra-S phase checkpoint monitoring replication origin firing [PDF]
, 2001 Checkpoints maintain order and fidelity in the cell cycle by blocking late-occurring events when earlier events are improperly executed. Here we describe evidence for the participation of Chk1 in an intra-S phase checkpoint in mammalian cells.
Feijoo, Carmen +6 more
core +2 more sources