Results 21 to 30 of about 2,048 (142)
DNA binding polarity, dimerization, and ATPase ring remodeling in the CMG helicase of the eukaryotic replisome [PDF]
eLife, 2014 The Cdc45/Mcm2-7/GINS (CMG) helicase separates DNA strands during replication in eukaryotes. How the CMG is assembled and engages DNA substrates remains unclear.
Alessandro Costa +9 more
doaj +2 more sources
BMC Cell Biology, 2009 Background Each of the three individual components of the CMG complex (Cdc45, MCM and GINS) is essential for chromosomal DNA replication in eukaryotic cells, both for the initiation of replication at origins and also for normal replication fork ...
MacNeill Stuart A, Akman Gökhan
doaj +2 more sources
The Eukaryotic CMG Helicase at the Replication Fork: Emerging Architecture Reveals an Unexpected Mechanism [PDF]
BioEssays, 2018 The eukaryotic helicase is an 11‐subunit machine containing an Mcm2‐7 motor ring that encircles DNA, Cdc45 and the GINS tetramer, referred to as CMG (Cdc45, Mcm2‐7, GINS). CMG is “built” on DNA at origins in two steps. First, two Mcm2‐7 rings are assembled around duplex DNA at origins in G1 phase, forming the Mcm2‐7 “double hexamer.” In a second step ...
Huilin Li, Mike O’Donnell
exaly +5 more sources
Dynamics of the Eukaryotic Replicative Helicase at Lagging-Strand Protein Barriers Support the Steric Exclusion Model [PDF]
Cell Reports, 2019 Summary: Progression of DNA replication depends on the ability of the replisome complex to overcome nucleoprotein barriers. During eukaryotic replication, the CMG helicase translocates along the leading-strand template and unwinds the DNA double helix ...
Hazal B. Kose +3 more
doaj +3 more sources
A Ctf4 trimer couples the CMG helicase to DNA polymerase α in the eukaryotic replisome [PDF]
Nature, 2014 Efficient duplication of the genome requires the concerted action of helicase and DNA polymerases at replication forks to avoid stalling of the replication machinery and consequent genomic instability. In eukaryotes, the physical coupling between helicase and DNA polymerases remains poorly understood. Here we define the molecular mechanism by which the
Simon, Aline C. +12 more
core +5 more sources
Mechanistic insights into how CMG helicase facilitates replication past DNA roadblocks [PDF]
DNA Repair, 2017 Before leaving the house, it is a good idea to check for road closures that may affect the morning commute. Otherwise, one may encounter significant delays arriving at the destination. While this is commonly true, motorists may be able to consult a live interactive traffic map and pick an alternate route or detour to avoid being late.
Michael A Trakselis +2 more
exaly +4 more sources
Cryo-EM snapshots of CMG helicase assembly
The accurate, complete and timely replication of DNA is essential for the propagation of life. Every proliferating cell needs to duplicate its entire genome before it can divide. At the heart of this process lies the replicative helicase MCM, which unwinds parental DNA, thereby providing the single-stranded template for replicative polymerases.
Pühringer, Thomas
openaire +2 more sources
The mechanism of DNA unwinding by the eukaryotic replicative helicase [PDF]
Nature Communications, 2019 How the eukaryotic helicase unzips DNA during replication is not well understood. By measuring the real-time motion of purified CMG unwinding DNA with magnetic tweezers, the authors reveal the dynamics where isolated CMG unwinds via a biased random walk ...
Daniel R. Burnham +3 more
doaj +3 more sources
Tethering of SCF(Dia2) to the Replisome Promotes Efficient Ubiquitylation and Disassembly of the CMG Helicase. [PDF]
Curr Biol, 2015 Disassembly of the Cdc45-MCM-GINS (CMG) DNA helicase, which unwinds the parental DNA duplex at eukaryotic replication forks, is the key regulated step during replication termination but is poorly understood. In budding yeast, the F-box protein Dia2 drives ubiquitylation of the CMG helicase at the end of replication, leading to a disassembly pathway ...
Maculins T +3 more
europepmc +6 more sources
Mcm10 regulates DNA replication elongation by stimulating the CMG replicative helicase
Genes & Development, 2017 Activation of the Mcm2–7 replicative DNA helicase is the committed step in eukaryotic DNA replication initiation. Although Mcm2–7 activation requires binding of the helicase-activating proteins Cdc45 and GINS (forming the CMG complex), an additional protein, Mcm10, drives initial origin DNA unwinding by an unknown mechanism.
Looke, Marko +2 more
openaire +5 more sources