CUL2<sup>LRR1</sup> , TRAIP and p97 control CMG helicase disassembly in the mammalian cell cycle. [PDF]
EMBO Rep, 2021 The eukaryotic replisome is disassembled in each cell cycle, dependent upon ubiquitylation of the CMG helicase. Studies of Saccharomyces cerevisiae, Caenorhabditis elegans and Xenopus laevis have revealed surprising evolutionary diversity in the ...
Villa F +6 more
europepmc +6 more sources
The Eukaryotic CMG Helicase at the Replication Fork: Emerging Architecture Reveals an Unexpected Mechanism [PDF]
BioEssays, 2018 The eukaryotic helicase is an 11‐subunit machine containing an Mcm2‐7 motor ring that encircles DNA, Cdc45 and the GINS tetramer, referred to as CMG (Cdc45, Mcm2‐7, GINS). CMG is “built” on DNA at origins in two steps. First, two Mcm2‐7 rings are assembled around duplex DNA at origins in G1 phase, forming the Mcm2‐7 “double hexamer.” In a second step ...
Huilin Li, Michael O'Donnell
exaly +5 more sources
MCM-GINS and MCM-MCM interactions
BMC Cell Biology, 2009 Background Each of the three individual components of the CMG complex (Cdc45, MCM and GINS) is essential for chromosomal DNA replication in eukaryotic cells, both for the initiation of replication at origins and also for normal replication fork ...
MacNeill Stuart A, Akman Gökhan
doaj +2 more sources
Regulation of CMG Helicase Disassembly During Replication Termination
, 2019 During DNA replication, the replicative helicase, CMG, unwinds the parental duplex at the leading edge of each replication fork. CMG unwinds DNA by encircling the leading strand template and excluding the lagging strand template, which interacts with the
Low, Emily
core +6 more sources
DNA binding polarity, dimerization, and ATPase ring remodeling in the CMG helicase of the eukaryotic replisome [PDF]
eLife, 2014 The Cdc45/Mcm2-7/GINS (CMG) helicase separates DNA strands during replication in eukaryotes. How the CMG is assembled and engages DNA substrates remains unclear.
Alessandro Costa +9 more
doaj +2 more sources
Cryo-EM snapshots of CMG helicase assembly
The accurate, complete and timely replication of DNA is essential for the propagation of life. Every proliferating cell needs to duplicate its entire genome before it can divide.
Pühringer, Thomas
core +2 more sources
Mechanistic insights into how CMG helicase facilitates replication past DNA roadblocks [PDF]
DNA Repair, 2017 Before leaving the house, it is a good idea to check for road closures that may affect the morning commute. Otherwise, one may encounter significant delays arriving at the destination. While this is commonly true, motorists may be able to consult a live interactive traffic map and pick an alternate route or detour to avoid being late.
Michael M Seidman +2 more
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A Ctf4 trimer couples the CMG helicase to DNA polymerase α in the eukaryotic replisome [PDF]
Nature, 2014 Efficient duplication of the genome requires the concerted action of helicase and DNA polymerases at replication forks to avoid stalling of the replication machinery and consequent genomic instability.
Evrin, Cecile +12 more
core +5 more sources
Unwinding the Role of the CMG Helicase in Inborn Errors of Immunity. [PDF]
J Clin Immunol, 2023 Guilz NC, Ahn YO, Seo S, Mace EM.
europepmc +3 more sources
CDT1 inhibits CMG helicase in early S phase to separate origin licensing from DNA synthesis [PDF]
Molecular Cell, 2023 Joseph T P Yeeles +2 more
exaly +2 more sources