The Role of the MCM2-7 Helicase Subunit MCM2 in Epigenetic Inheritance [PDF]
BiologyRecycling histone proteins from parental chromatin, a process known as parental histone transfer, is an important component in chromosome replication and is essential for epigenetic inheritance.
Chuanhe Yu, Yu Chuanhe
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Cryo-EM structure of human hexameric MCM2-7 complex [PDF]
iScience, 2022 Summary: The central step in the initiation of eukaryotic DNA replication is the loading of the minichromosome maintenance 2–7 (MCM2-7) complex, the core of the replicative DNA helicase, onto chromatin at replication origin. Here, we reported the cryo-EM
Naining Xu +8 more
doaj +7 more sources
Assembly, Activation, and Helicase Actions of MCM2-7: Transition from Inactive MCM2-7 Double Hexamers to Active Replication Forks [PDF]
BiologyIn this review, we summarize the processes of the assembly of multi-protein replisomes at the origins of replication. Replication licensing, the loading of inactive minichromosome maintenance double hexamers (dhMCM2-7) during the G1 phase, is followed by
Zhiying You, Hisao Masai, Masai Hisao
exaly +7 more sources
MCM2-7 in Clear Cell Renal Cell Carcinoma: MCM7 Promotes Tumor Cell Proliferation [PDF]
Frontiers in Oncology, 2021 BackgroundClear cell renal cell carcinoma (ccRCC) accounts for 60-70% of renal cell carcinoma (RCC) cases. Finding more therapeutic targets for advanced ccRCC is an urgent mission.
Qingshui Wang, Wang Qingshui
exaly +5 more sources
MCM2-7 ring closure involves the Mcm5 C-terminus and triggers Mcm4 ATP hydrolysis [PDF]
Nature CommunicationsThe eukaryotic helicase MCM2-7, is loaded by ORC, Cdc6 and Cdt1 as a double-hexamer onto replication origins. The insertion of DNA into the helicase leads to partial MCM2-7 ring closure, while ATP hydrolysis is essential for consecutive steps in pre ...
Sarah V. Faull +11 more
doaj +4 more sources
DDK regulates replication initiation by controlling the multiplicity of Cdc45-GINS binding to Mcm2-7 [PDF]
eLife, 2021 The committed step of eukaryotic DNA replication occurs when the pairs of Mcm2-7 replicative helicases that license each replication origin are activated. Helicase activation requires the recruitment of Cdc45 and GINS to Mcm2-7, forming Cdc45-Mcm2-7-GINS
Lorraine De Jesús-Kim +5 more
doaj +4 more sources
MCM2-7 loading-dependent ORC release ensures genome-wide origin licensing [PDF]
Nature CommunicationsOrigin recognition complex (ORC)-dependent loading of the replicative helicase MCM2-7 onto replication origins in G1-phase forms the basis of replication fork establishment in S-phase.
L. Maximilian Reuter +6 more
doaj +5 more sources
MCM2-7 complex is a novel druggable target for neuroendocrine prostate cancer [PDF]
Scientific Reports, 2021 Neuroendocrine prostate cancer (NEPC) is a lethal subtype of prostate cancer that rarely develops de novo in primary tumors and is commonly acquired during the development of treatment resistance.
En-Chi Hsu +11 more
doaj +6 more sources
MCMBP promotes the assembly of the MCM2–7 hetero-hexamer to ensure robust DNA replication in human cells [PDF]
eLife, 2022 The MCM2–7 hetero-hexamer is the replicative DNA helicase that plays a central role in eukaryotic DNA replication. In proliferating cells, the expression level of the MCM2–7 hexamer is kept high, which safeguards the integrity of the genome. However, how
Yuichiro Saito +3 more
doaj +4 more sources
The structural basis of Cdc7-Dbf4 kinase dependent targeting and phosphorylation of the MCM2-7 double hexamer [PDF]
Nature Communications, 2022 Here the authors describe multiple structures of the replicative helicase MCM2-7 in complex with Dbf4-dependent kinase (DDK). These structures reveal why the kinase specifically recognizes the MCM2-7 double-hexamer over the single-hexamer and explain how
Almutasem Saleh +9 more
doaj +3 more sources