Cryo-EM structure of Mcm2-7 double hexamer on DNA suggests a lagging-strand DNA extrusion model. [PDF]
Proc Natl Acad Sci U S A, 2017 Significance During initiation of DNA replication in eukaryotes, the origin recognition complex, with Cdc6 and Cdt1, assembles an inactive Mcm2-7 double hexamer on the dsDNA.
Noguchi Y +7 more
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Structural insights into the Cdt1-mediated MCM2–7 chromatin loading [PDF]
Nucleic Acids Research, 2011 Initiation of DNA replication in eukaryotes is exquisitely regulated to ensure that DNA replication occurs exactly once in each cell division. A conserved and essential step for the initiation of eukaryotic DNA replication is the loading of the mini-chromosome maintenance 2-7 (MCM2-7) helicase onto chromatin at replication origins by Cdt1. To elucidate
Liu, Changdong LIFS +7 more
openaire +4 more sources
Binding of Human MCM-BP with MCM2-7 Proteins [PDF]
Genes & Development, 2011 MCM2-7 proteins play essential roles in DNA replication in eukaryotic cells, probably by acting as a replicative DNA helicase that unwinds DNA duplexes at replication forks (Bell & Dutta, 2002; Forsburg, 2004; Masai et al., 2010). Several lines of evidences suggest that MCM2-7 hexameric complexes assembled on the replication origins are converted to an
Ryoko Kon +3 more
core +5 more sources
Chronic DNA Replication Stress Reduces Replicative Lifespan of Cells by TRP53-Dependent, microRNA-Assisted MCM2-7 Downregulation. [PDF]
PLoS Genetics, 2016 Circumstances that compromise efficient DNA replication, such as disruptions to replication fork progression, cause a state known as DNA replication stress (RS).
Gongshi Bai +2 more
doaj +2 more sources
Multiple functions for Mcm2-7 ATPase motifs during replication initiation. [PDF]
Mol Cell, 2014 The Mcm2-7 replicative helicase is central to all steps of eukaryotic DNA replication. The hexameric ring of Mcm subunits forms six essential ATPases whose contributions to replication initiation remain unclear. Mcm2-7 complexes containing ATPase-motif mutations showed Mcm2-7 ATP binding and hydrolysis are required for helicase loading.
Kang S, Warner MD, Bell SP.
europepmc +7 more sources
Structural and mechanistic insights into Mcm2-7 double-hexamer assembly and function. [PDF]
Genes Dev, 2014 Eukaryotic cells license each DNA replication origin during G1 phase by assembling a prereplication complex that contains a Mcm2–7 (minichromosome maintenance proteins 2–7) double hexamer. During S phase, each Mcm2–7 hexamer forms the core of a replicative DNA helicase.
Sun J +7 more
europepmc +7 more sources
Targeting CDC7 sensitizes resistance melanoma cells to BRAFV600E-specific inhibitor by blocking the CDC7/MCM2-7 pathway. [PDF]
Sci Rep, 2019 Although the utilization of selective BRAFV600E inhibitors is associated with improved overall survival in patients with metastatic melanoma, a growing challenge of drug resistance has emerged.
Gad SA +9 more
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Odontoestomatología, 2019 La formación de nuevas células se genera a partir de células preexistentes a través de una serie ordenada de eventos denominada ciclo celular. El control de este es fundamental para la integridad del genoma, por lo que hay múltiples proteínas regulando ...
Delmira Apellaniz +3 more
doaj +1 more source
An intact Mcm10 coiled-coil interaction surface is important for origin melting, helicase assembly and the recruitment of Pol-α to Mcm2-7. [PDF]
Nucleic Acids Res, 2017 Mcm10 is an essential eukaryotic factor required for DNA replication. The replication fork helicase is composed of Cdc45, Mcm2–7 and GINS (CMG). DDK is an S-phase-specific kinase required for replication initiation, and the DNA primase-polymerase in ...
Perez-Arnaiz P +3 more
europepmc +2 more sources
TIMELESS Suppresses the Accumulation of Aberrant CDC45·MCM2-7·GINS Replicative Helicase Complexes on Human Chromatin. [PDF]
J Biol Chem, 2016 The replication licensing factor CDC6 recruits the MCM2-7 replicative helicase to the replication origin, where MCM2-7 is activated to initiate DNA replication.
Xu X, Wang JT, Li M, Liu Y.
europepmc +2 more sources