MCM2-7 conformational changes in the presence of DNA. [PDF]
Cell Cycle, 2016 [no abstract]
Schaffitzel C.
europepmc +4 more sources
The ORC/Cdc6/MCM2-7 complex facilitates MCM2-7 dimerization during prereplicative complex formation. [PDF]
Nucleic Acids Res, 2014 AbstractThe replicative mini-chromosome-maintenance 2–7 (MCM2-7) helicase is loaded in Saccharomyces cerevisiae and other eukaryotes as a head-to-head double-hexamer around origin DNA. At first, ORC/Cdc6 recruits with the help of Cdt1 a single MCM2-7 hexamer to form an ‘initial’ ORC/Cdc6/Cdt1/MCM2-7 complex.
Evrin C +8 more
europepmc +7 more sources
The N-terminus of Spt16 anchors FACT to MCM2-7 for parental histone recycling. [PDF]
Nucleic Acids Res, 2023 Abstract Parental histone recycling is vital for maintaining chromatin-based epigenetic information during replication, yet its underlying mechanisms remain unclear. Here, we uncover an unexpected role of histone chaperone FACT and its N-terminus of the Spt16 subunit during parental histone recycling and transfer in budding yeast ...
Wang X +9 more
europepmc +3 more sources
The Mcm2-7 replicative helicase: a promising chemotherapeutic target. [PDF]
Biomed Res Int, 2014 Numerous eukaryotic replication factors have served as chemotherapeutic targets. One replication factor that has largely escaped drug development is the Mcm2-7 replicative helicase. This heterohexameric complex forms the licensing system that assembles the replication machinery at origins during initiation, as well as the catalytic core of the CMG ...
Simon NE, Schwacha A.
europepmc +5 more sources
Origin single-stranded DNA releases Sld3 protein from the Mcm2-7 complex, allowing the GINS tetramer to bind the Mcm2-7 complex. [PDF]
J Biol Chem, 2017 The replication fork helicase in eukaryotic cells is comprised of Cdc45, Mcm2-7, and GINS (CMG complex). In budding yeast, Sld3, Sld2, and Dpb11 are required for the initiation of DNA replication, but Sld3 and Dpb11 do not travel with the replication fork. Sld3 and Cdc45 bind to early replication origins during the G(1) phase of the cell cycle, whereas
Bruck I, Kaplan DL.
europepmc +6 more sources
A helicase-tethered ORC flip enables bidirectional helicase loading
eLife, 2021 Replication origins are licensed by loading two Mcm2-7 helicases around DNA in a head-to-head conformation poised to initiate bidirectional replication. This process requires origin–recognition complex (ORC), Cdc6, and Cdt1.
Shalini Gupta +3 more
doaj +1 more source
Proteogenomic characterization of cholangiocarcinoma
Hepatology, EarlyView., 2022 Proteogenomic characterization of cholangiocarcinoma with therapeutic strategies Abstract Background and Aims Cholangiocarcinoma (CCA) is a highly heterogeneous cancer with limited understanding and few effective therapeutic approaches. We aimed at providing a proteogenomic CCA characterization to inform biological processes and treatment ...
Mengjie Deng +18 more
wiley +1 more source
Role of MCM2-7 protein phosphorylation in human cancer cells. [PDF]
Cell Biosci, 2018 A heterohexameric complex composed of minichromosome maintenance protein 2-7 (MCM2-7), which acts as a key replicative enzyme in eukaryotes, is crucial for initiating DNA synthesis only once per cell cycle. The MCM complex remains inactive through the G1 phase, until the S phase, when it is activated to initiate replication.
Fei L, Xu H.
europepmc +5 more sources
In the absence of ATPase activity, pre-RC formation is blocked prior to MCM2–7 hexamer dimerization [PDF]
, 2013 26.03.14 KB.
Brill, S +8 more
core +2 more sources
Mcm2 promotes stem cell differentiation via its ability to bind H3-H4
eLife, 2022 Mcm2, a subunit of the minichromosome maintenance proteins 2–7 (Mcm2-7) helicase best known for its role in DNA replication, contains a histone binding motif that facilitates the transfer of parental histones following DNA replication. Here, we show that
Xiaowei Xu +4 more
doaj +1 more source