Results 51 to 60 of about 7,568,861 (163)
Mcm2-, Mcm4-, and Mcm6-ChEC footprints over 12 origins on chrIV.
, 2021 Footprints for Mcm2-ChEC (top row), Mcm4-ChEC (second row), and Mcm6-ChEC (third row) resemble each other at each of 12 consecutive origins on chrIV, but free MNase (bottom row) does not.
Tonibelle Gatbonton-Schwager (5707766) +6 more
core +1 more source
Modulation of Mcm2–7 activity by Cdt1
The FASEB Journal, 2013 Genome duplication occurs once and only once in each cell cycle. During G1 phase, pre‐replicative complexes (pre‐RC), composed of ORC, Cdc6, Cdt1 and Mcm2–7, are assembled at replication origins. Mcm2–7 is the replicative helicase in eukaryotic cells; however, it does not unwind DNA in the pre‐RC.
Lance F DaSilva +3 more
openaire +1 more source
Post-transcriptional homeostasis and regulation of MCM2–7 in mammalian cells [PDF]
Nucleic Acids Research, 2012 The MiniChromosome Maintenance 2-7 (MCM2-7) complex provides essential replicative helicase function. Insufficient MCMs impair the cell cycle and cause genomic instability (GIN), leading to cancer and developmental defects in mice. Remarkably, depletion or mutation of one Mcm can decrease all Mcm levels.
Chuang, Chen-Hua +5 more
openaire +2 more sources
Cryo-EM structure of a helicase loading intermediate containing ORC-Cdc6-Cdt1-MCM2-7 bound to DNA [PDF]
, 2013 26.03.14 KB.
Cecile Evrin +26 more
core +1 more source
BMC Biology, 2021 Background Faithful DNA replication is essential to maintain genomic stability in all living organisms, and the regulatory pathway for DNA replication initiation is conserved from yeast to humans.
Kyu Joon Lee, Ziyin Li
doaj +1 more source
The structural basis for MCM2–7 helicase activation by GINS and Cdc45 [PDF]
Nature Structural & Molecular Biology, 2011 Two central steps for initiating eukaryotic DNA replication involve loading of the Mcm2-7 helicase onto double-stranded DNA and its activation by GINS-Cdc45. To better understand these events, we determined the structures of Mcm2-7 and the CMG complex by using single-particle electron microscopy.
Alessandro, Costa +6 more
openaire +2 more sources
The PS1 hairpin of Mcm3 is essential for viability and for DNA unwinding in vitro. [PDF]
PLoS ONE, 2013 The pre-sensor 1 (PS1) hairpin is found in ring-shaped helicases of the AAA+ family (ATPases associated with a variety of cellular activities) of proteins and is implicated in DNA translocation during DNA unwinding of archaeal mini-chromosome maintenance
Simon K W Lam +5 more
doaj +1 more source
Human GINS, a conserved DNA replication factor and candidate cancer marker [PDF]
, 2011 The GINS complex (a heterotetramer of Sld5, Psf1, Psf2 and Psf3) is a highly conserved DNA replication factor required for the initiation and elongation of DNA replication. GINS is believed to associate with Cdc45 and MCM proteins on replicating DNA. The
core +2 more sources
Antagonistic control of DDK binding to licensed replication origins by Mcm2 and Rad53
eLife, 2020 Eukaryotic replication origins are licensed by the loading of the replicative DNA helicase, Mcm2-7, in inactive double hexameric form around DNA. Subsequent origin activation is under control of multiple protein kinases that either promote or inhibit ...
Syafiq Abd Wahab, Dirk Remus
doaj +1 more source
Genes & Genetic Systems, 2018 Recently published structural and functional analyses of the CMG complex have provided insight into the mechanism of its DNA helicase function and into the distinct roles of its central six component proteins MCM2-MCM7 (MCM2-7). To activate CMG helicase, the two protein kinases CDK and DDK, as well as MCM10, are required.
openaire +3 more sources