Results 41 to 50 of about 7,568,861 (163)
The Mcm2-7 Complex Has In Vitro Helicase Activity [PDF]
Molecular Cell, 2008 Helicases unwind duplex DNA ahead of the polymerases at the replication fork. However, the identity of the eukaryotic replicative helicase has been controversial; in vivo studies implicate the ring-shaped heterohexameric Mcm2-7 complex, although only a specific subset of Mcm subunits (Mcm467) unwind DNA in vitro.
Bochman, Matthew L., Schwacha, Anthony
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Chronic DNA Replication Stress Reduces Replicative Lifespan of Cells by TRP53-Dependent, microRNA-Assisted MCM2-7 Downregulation. [PDF]
PLoS Genetics, 2016 Circumstances that compromise efficient DNA replication, such as disruptions to replication fork progression, cause a state known as DNA replication stress (RS).
Gongshi Bai +2 more
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Stepwise Regulated Chromatin Assembly of MCM2–7 Proteins [PDF]
Journal of Biological Chemistry, 2000 Acquisition of the competence to replicate requires the assembly of the MCM2-7 (minichromosome maintenance) protein complex onto pre-replicative chromatin, a step of the licensing reaction. This step is thought to occur through binding of a heterohexameric MCM complex containing the six related MCM subunits.
D, Maiorano, J M, Lemaître, M, Méchali
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PLoS Genetics, 2022 The MCM2-7 complex is a highly conserved hetero-hexameric protein complex, critical for DNA unwinding at the replicative fork during DNA replication. Overexpression or mutation in MCM2-7 genes is linked to and may drive several cancer types in humans. In
Irene Rubio-Ferrera +7 more
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Decreased MCM2-6 in Drosophila S2 cells does not generate significant DNA damage or cause a marked increase in sensitivity to replication interference. [PDF]
, 2011 A reduction in the level of some MCM proteins in human cancer cells (MCM5 in U20S cells or MCM3 in Hela cells) causes a rapid increase in the level of DNA damage under normal conditions of cell proliferation and a loss of viability when the cells are ...
Cotterill, S. +24 more
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Mechanism and timing of Mcm2-7 ring closure during DNA replication origin licensing. [PDF]
Nat Struct Mol Biol, 2017 The opening and closing of two ring-shaped Mcm2-7 DNA helicases is necessary to license eukaryotic origins of replication, although the mechanisms controlling these events are unclear.
Ticau S +5 more
europepmc +2 more sources
SLAS Discovery, 2022 Mcm2-7 is the catalytic core of the eukaryotic replicative helicase, which together with CDC45 and the GINS complex unwind parental DNA to generate templates for DNA polymerase.
Jordan Sanders +6 more
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Activation of the MCM2-7 Helicase by Association with Cdc45 and GINS Proteins [PDF]
Molecular Cell, 2010 MCM2-7 proteins provide essential helicase functions in eukaryotes at chromosomal DNA replication forks. During the G1 phase of the cell cycle, they remain loaded on DNA but are inactive. We have used recombinant methods to show that the Drosophila MCM2-7 helicase is activated in complex with Cdc45 and the four GINS proteins (CMG complex).
Ilves, Ivar +3 more
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Nature Communications, 2020 The origin recognition complex (ORC) is essential for loading the Mcm2–7 replicative helicase onto DNA during DNA replication initiation. Here, the authors describe several cryo-electron microscopy structures of Drosophila ORC bound to DNA and its ...
Jan Marten Schmidt, Franziska Bleichert
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Expression and Prognostic Value of MCM Family Genes in Osteosarcoma
Frontiers in Molecular Biosciences, 2021 We performed a detailed cancer VS normal analysis to explore the expression and prognostic value of minichromosome maintenance (MCM) proteinsin human sarcoma.
Jian Zhou +6 more
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