The Mcm2-7-interacting domain of human mini-chromosome maintenance 10 (Mcm10) protein is important for stable chromatin association and origin firing. [PDF]
J Biol Chem, 2017 The protein mini-chromosome maintenance 10 (Mcm10) was originally identified as an essential yeast protein in the maintenance of mini-chromosome plasmids.
Izumi M +7 more
europepmc +2 more sources
Single-Molecule Visualization of MCM2-7 DNA Loading: Seeing Is Believing [PDF]
Cell, 2015 The first event in the initiation of eukaryotic DNA replication is the recruitment of the MCM2-7 ATPase, the core of the replicative DNA helicase, to origins. Ticau et al. use single-molecule imaging to reveal how ORC, Cdc6, and Cdt1 cooperate to load MCM2-7 onto DNA, enabling bidirectional replication.
Chistol, Gheorghe, Walter, Johannes C.
openaire +3 more sources
The ORC/Cdc6/MCM2-7 complex facilitates MCM2-7 dimerization during prereplicative complex formation. [PDF]
Nucleic Acids Res, 2014 AbstractThe replicative mini-chromosome-maintenance 2–7 (MCM2-7) helicase is loaded in Saccharomyces cerevisiae and other eukaryotes as a head-to-head double-hexamer around origin DNA. At first, ORC/Cdc6 recruits with the help of Cdt1 a single MCM2-7 hexamer to form an ‘initial’ ORC/Cdc6/Cdt1/MCM2-7 complex.
Evrin C +8 more
europepmc +7 more sources
MCM2-7 conformational changes in the presence of DNA. [PDF]
Cell Cycle, 2016 [no abstract]
Schaffitzel C.
europepmc +5 more sources
Pre-initiation complex assembly functions as a molecular switch that splits the Mcm2-7 double hexamer. [PDF]
EMBO Rep, 2017 Miyazawa-Onami M, Araki H, Tanaka S.
europepmc +2 more sources
The Mcm2-7 replicative helicase: a promising chemotherapeutic target. [PDF]
Biomed Res Int, 2014 Numerous eukaryotic replication factors have served as chemotherapeutic targets. One replication factor that has largely escaped drug development is the Mcm2-7 replicative helicase. This heterohexameric complex forms the licensing system that assembles the replication machinery at origins during initiation, as well as the catalytic core of the CMG ...
Simon NE, Schwacha A.
europepmc +5 more sources
Origin single-stranded DNA releases Sld3 protein from the Mcm2-7 complex, allowing the GINS tetramer to bind the Mcm2-7 complex. [PDF]
J Biol Chem, 2017 The replication fork helicase in eukaryotic cells is comprised of Cdc45, Mcm2-7, and GINS (CMG complex). In budding yeast, Sld3, Sld2, and Dpb11 are required for the initiation of DNA replication, but Sld3 and Dpb11 do not travel with the replication fork. Sld3 and Cdc45 bind to early replication origins during the G(1) phase of the cell cycle, whereas
Bruck I, Kaplan DL.
europepmc +6 more sources
A helicase-tethered ORC flip enables bidirectional helicase loading
eLife, 2021 Replication origins are licensed by loading two Mcm2-7 helicases around DNA in a head-to-head conformation poised to initiate bidirectional replication. This process requires origin–recognition complex (ORC), Cdc6, and Cdt1.
Shalini Gupta +3 more
doaj +1 more source
Molecular architecture of the recombinant human MCM2-7 helicase in complex with nucleotides and DNA. [PDF]
Cell Cycle, 2016 Boskovic J +7 more
europepmc +2 more sources
Proteogenomic characterization of cholangiocarcinoma
Hepatology, EarlyView., 2022 Proteogenomic characterization of cholangiocarcinoma with therapeutic strategies Abstract Background and Aims Cholangiocarcinoma (CCA) is a highly heterogeneous cancer with limited understanding and few effective therapeutic approaches. We aimed at providing a proteogenomic CCA characterization to inform biological processes and treatment ...
Mengjie Deng +18 more
wiley +1 more source