O-GlcNAc transferase associates with the MCM2-7 complex and its silencing destabilizes MCM-MCM interactions. [PDF]
Cell Mol Life Sci, 2018 O-GlcNAcylation of proteins is governed by O-GlcNAc transferase (OGT) and O-GlcNAcase (OGA). The homeostasis of O-GlcNAc cycling is regulated during cell cycle progression and is essential for proper cellular division.
Leturcq M +5 more
europepmc +4 more sources
Regulation of replication origin licensing by ORC phosphorylation reveals a two-step mechanism for Mcm2-7 ring closing. [PDF]
Proc Natl Acad Sci U S A, 2023 Eukaryotic DNA replication must occur exactly once per cell cycle to maintain cell ploidy. This outcome is ensured by temporally separating replicative helicase loading (G1 phase) and activation (S phase).
Amasino AL +4 more
europepmc +3 more sources
The N-terminus of Spt16 anchors FACT to MCM2-7 for parental histone recycling. [PDF]
Nucleic Acids Res, 2023 Parental histone recycling is vital for maintaining chromatin-based epigenetic information during replication, yet its underlying mechanisms remain unclear.
Wang X +9 more
europepmc +2 more sources
Targeting KIF23 inhibits cell proliferation and primary chemoresistance in cervical cancer by inactivating the MYH9/MCM2/PCNA pathway [PDF]
Clinical and Translational MedicineBackground Kinesin family member 23 (KIF23) is recognised as an important tumour promoter involved in the pathogenesis of various cancers. However, its role and underlying molecular mechanisms in regulating cervical cancer (CC) growth and primary ...
Ying Zhu +7 more
doaj +3 more sources
Chromosomal Mcm2-7 distribution and the genome replication program in species from yeast to humans. [PDF]
PLoS Genet, 2021 The spatio-temporal program of genome replication across eukaryotes is thought to be driven both by the uneven loading of pre-replication complexes (pre-RCs) across the genome at the onset of S-phase, and by differences in the timing of activation of ...
Foss EJ +6 more
europepmc +2 more sources
Specific origin selection and excess functional MCM2-7 loading in ORC-deficient cells. [PDF]
Nucleic Acids ResThe six-subunit origin recognition complex (ORC) loads excess MCM2–7 on chromosomes to promote initiation of DNA replication and is believed to be important for origin specification.
Shibata Y +5 more
europepmc +3 more sources
Ciprofloxacin is an inhibitor of the Mcm2-7 replicative helicase. [PDF]
Biosci Rep, 2013 Most currently available small molecule inhibitors of DNA replication lack enzymatic specificity, resulting in deleterious side effects during use in cancer chemotherapy and limited experimental usefulness as mechanistic tools to study DNA replication.
Simon N +5 more
europepmc +6 more sources
Sir2 suppresses transcription-mediated displacement of Mcm2-7 replicative helicases at the ribosomal DNA repeats. [PDF]
PLoS Genet, 2019 Repetitive DNA sequences within eukaryotic heterochromatin are poorly transcribed and replicate late in S-phase. In Saccharomyces cerevisiae, the histone deacetylase Sir2 is required for both transcriptional silencing and late replication at the ...
Foss EJ +7 more
europepmc +2 more sources
Differential requirements for MCM proteins in DNA replication in Drosophila S2 cells. [PDF]
PLoS ONE, 2007 The MCM2-7 proteins are crucial components of the pre replication complex (preRC) in eukaryotes. Since they are significantly more abundant than other preRC components, we were interested in determining whether the entire cellular content was necessary ...
Gilles Crevel +6 more
doaj +3 more sources
Regulation of MCM2-7 function.
Genes & Genetic Systems, 2018 Recently published structural and functional analyses of the CMG complex have provided insight into the mechanism of its DNA helicase function and into the distinct roles of its central six component proteins MCM2-MCM7 (MCM2-7). To activate CMG helicase,
Y. Ishimi
semanticscholar +4 more sources