Results 21 to 30 of about 7,568,861 (163)

Structural basis of Mcm2-7 replicative helicase loading by ORC-Cdc6 and Cdt1. [PDF]

Nat Struct Mol Biol, 2017
To initiate DNA replication, the origin recognition complex (ORC) and Cdc6 load an Mcm2-7 double hexamer onto DNA. Without ATP hydrolysis, ORC-Cdc6 recruits one Cdt1-bound Mcm2-7 hexamer, thus forming an ORC-Cdc6-Cdt1-Mcm2-7 (OCCM) helicase-loading intermediate. Here we report a 3.9-Å structure of Saccharomyces cerevisiae OCCM on DNA.
Yuan Z, Riera A, Bai L, Sun J, Nandi S, Spanos C, Chen ZA, Barbon M, Rappsilber J, Stillman B, Speck C, Li H.   +11 more
europepmc   +10 more sources

Multiple functions for Mcm2-7 ATPase motifs during replication initiation. [PDF]

Mol Cell, 2014
The Mcm2-7 replicative helicase is central to all steps of eukaryotic DNA replication. The hexameric ring of Mcm subunits forms six essential ATPases whose contributions to replication initiation remain unclear. Mcm2-7 complexes containing ATPase-motif mutations showed Mcm2-7 ATP binding and hydrolysis are required for helicase loading.
Kang S, Warner MD, Bell SP.
europepmc   +8 more sources

Complejo mcm2-7.

Odontoestomatología, 2019
La formación de nuevas células se genera a partir de células preexistentes a través de una serie ordenada de eventos denominada ciclo celular. El control de este es fundamental para la integridad del genoma, por lo que hay múltiples proteínas regulando ...
Delmira Apellaniz, Vanesa Pereira-Prado, Gabriel Tapia-Repetto, Ronell Bologna-Molina   +3 more
doaj   +1 more source

Structural and mechanistic insights into Mcm2-7 double-hexamer assembly and function. [PDF]

Genes Dev, 2014
Eukaryotic cells license each DNA replication origin during G1 phase by assembling a prereplication complex that contains a Mcm2–7 (minichromosome maintenance proteins 2–7) double hexamer. During S phase, each Mcm2–7 hexamer forms the core of a replicative DNA helicase.
Sun J, Fernandez-Cid A, Riera A, Tognetti S, Yuan Z, Stillman B, Speck C, Li H.   +7 more
europepmc   +7 more sources

Structural insights into Sld3-Sld7-dependent Cdc45 loading during replication initiation [PDF]

Nature Communications
Regulated helicase activation by DDK kinase is central for genome stability. However, how DDK phosphorylation primes the MCM2-7 double hexamer (DH) for Sld3-Sld7 binding and Cdc45 loading remained unclear.
Yasunori Noguchi, Almutasem Saleh, Sarah Schneider, Marina E. Ivanova, Zhuo Angel Chen, Lepakshi Ranjha, Ricardo Aramayo, Silvia Tognetti, Sarah V. Faull, Juri Rappsilber, Christian Speck   +10 more
doaj   +2 more sources

Structure of the MCM2-7 Double Hexamer and Its Implications for the Mechanistic Functions of the Mcm2-7 Complex

, 2017
The eukaryotic minichromosome maintenance 2-7 complex is the core of the inactive MCM replication licensing complex and the catalytic core of the Cdc45-MCM-GINS replicative helicase. The years of effort to determine the structure of parts or the whole of the heterohexameric complex by X-ray crystallography and conventional cryo-EM produced limited ...
Zhai, Yuanliang, Tye, Bik Kwoon YEUNG
core   +5 more sources

An investigation into minichromosomal maintenance proteins (MCMs) for the diagnosis of prostate cancer, as a possible alternative to prostate specific antigen (PSA) [PDF]

, 2005
The current strategy for the diagnosis of prostate cancer includes serum prostate specific antigen (PSA) measurement. There is however debate into its specificity and sensitivity, so new diagnostic markers are under investigation.
Watkins, Jane Louise
core   +7 more sources

MCM2-7 Form Double Hexamers at Licensed Origins in Xenopus Egg Extract [PDF]

Journal of Biological Chemistry, 2011
In late mitosis and G1, Mcm2-7 are assembled onto replication origins to license them for initiation in the upcoming S phase. After initiation, Mcm2-7 provide helicase activity to unwind DNA at the replication fork. Here we examine the structure of Mcm2-7 on chromatin in Xenopus egg extracts.
Gambus, Agnieszka, Khoudoli, Guennadi A., Jones, Richard C., Blow, J. Julian   +3 more
openaire   +4 more sources

The Roles of the Mcm2-7 Tails in Replication Initiation

, 2022
DNA replication is complex biological reaction that spans two stages of the eukaryotic cell cycle. In G1 phase, preparation for replication begins when two copies of the replicative helicase, the Mcm2-7 complex, are loaded onto DNA. However, these helicases are loaded in an inactive state, and are not activated until the following S phase.
Gangaramani, Paritosh
openaire   +2 more sources

The assembly of the MCM2-7 hetero-hexamer and its significance in DNA replication. [PDF]

Biochem Soc Trans, 2023
The mini-chromosome maintenance proteins 2–7 (MCM2–7) hexamer is a protein complex that is key for eukaryotic DNA replication, which occurs only once per cell cycle. To achieve DNA replication, eukaryotic cells developed multiple mechanisms that control the timing of the loading of the hexamer onto chromatin and its activation as the replicative ...
Hatoyama Y, Kanemaki MT.
europepmc   +3 more sources