Ciprofloxacin is an inhibitor of the Mcm2-7 replicative helicase [PDF]
Bioscience Reports, 2013 Most currently available small molecule inhibitors of DNA replication lack enzymatic specificity, resulting in deleterious side effects during use in cancer chemotherapy and limited experimental usefulness as mechanistic tools to study DNA replication ...
Anthony Schwacha +5 more
doaj +7 more sources
Complejo mcm2-7. Revisión bibliográfica [PDF]
Odontoestomatología, 2018 Resumen: La formación de nuevas células se genera a partir de células preexistentes a través de una serie ordenada de eventos denominada ciclo celular.
Delmira Apellániz +3 more
doaj +5 more sources
MCM2-7 loading-dependent ORC release ensures genome-wide origin licensing [PDF]
Nature CommunicationsOrigin recognition complex (ORC)-dependent loading of the replicative helicase MCM2-7 onto replication origins in G1-phase forms the basis of replication fork establishment in S-phase.
L. Maximilian Reuter +6 more
doaj +3 more sources
Distinct roles of MCM2-7 subunits in replication licensing in human cells [PDF]
Nature CommunicationsEukaryotic DNA replication requires the precise assembly of MCM2-7 single hexamers (SHs) into head-to-head double hexamers (DHs) at replication origins.
Xinyu Fan +15 more
doaj +2 more sources
A conserved Mcm4 motif is required for Mcm2-7 double-hexamer formation and origin DNA unwinding [PDF]
eLife, 2019 Licensing of eukaryotic origins of replication requires DNA loading of two copies of the Mcm2-7 replicative helicase to form a head-to-head double-hexamer, ensuring activated helicases depart the origin bidirectionally.
Kanokwan Champasa +4 more
doaj +2 more sources
MCM2–7-dependent cohesin loading during S phase promotes sister-chromatid cohesion [PDF]
eLife, 2018 DNA replication transforms cohesin rings dynamically associated with chromatin into the cohesive form to establish sister-chromatid cohesion. Here, we show that, in human cells, cohesin loading onto chromosomes during early S phase requires the ...
Ge Zheng +3 more
doaj +2 more sources
Differential requirements for MCM proteins in DNA replication in Drosophila S2 cells. [PDF]
PLoS ONE, 2007 The MCM2-7 proteins are crucial components of the pre replication complex (preRC) in eukaryotes. Since they are significantly more abundant than other preRC components, we were interested in determining whether the entire cellular content was necessary ...
Gilles Crevel +6 more
doaj +3 more sources
Structural insights into the Cdt1-mediated MCM2–7 chromatin loading [PDF]
Nucleic Acids Research, 2011 Initiation of DNA replication in eukaryotes is exquisitely regulated to ensure that DNA replication occurs exactly once in each cell division. A conserved and essential step for the initiation of eukaryotic DNA replication is the loading of the mini-chromosome maintenance 2-7 (MCM2-7) helicase onto chromatin at replication origins by Cdt1. To elucidate
Liu, Changdong LIFS +7 more
openaire +4 more sources
ITRAQ-based quantitative proteomic analysis reveals that VPS35 promotes the expression of MCM2-7 genes in HeLa cells [PDF]
Scientific Reports, 2022 Vacuolar protein sorting 35 (VPS35) is a major component of the retromer complex that regulates endosomal trafficking in eukaryotic cells. Recent studies have shown that VPS35 promotes tumor cell proliferation and affects the nuclear accumulation of its ...
Xian Hong +9 more
doaj +2 more sources
Binding of Human MCM-BP with MCM2-7 Proteins [PDF]
Genes & Development, 2011 MCM2-7 proteins play essential roles in DNA replication in eukaryotic cells, probably by acting as a replicative DNA helicase that unwinds DNA duplexes at replication forks (Bell & Dutta, 2002; Forsburg, 2004; Masai et al., 2010). Several lines of evidences suggest that MCM2-7 hexameric complexes assembled on the replication origins are converted to an
Ryoko Kon +3 more
core +5 more sources