Results 1 to 10 of about 27,583 (169)

Replisomes restrict SMC translocation in vivo [PDF]

Nature Communications
Structural maintenance of chromosomes (SMC) complexes organize genomes by extruding DNA loops, while replisomes duplicate entire chromosomes. These essential molecular machines must collide frequently in every cell cycle, yet how such collisions are ...
Qin Liao, Hugo B. Brandão, Zhongqing Ren, Xindan Wang   +3 more
doaj   +4 more sources

Parental Histone Recycling During Chromatin Replication [PDF]

Biomolecules
The past decade has seen significant advancement in our understanding of DNA replication-coupled chromatin assembly, especially parental histone recycling that is essential for epigenetic inheritance.
Xin Bi
doaj   +2 more sources

Coordinated DNA Replication by the Bacteriophage T4 Replisome

Viruses, 2015
The T4 bacteriophage encodes eight proteins, which are sufficient to carry out coordinated leading and lagging strand DNA synthesis. These purified proteins have been used to reconstitute DNA synthesis in vitro and are a well-characterized model system ...
Stephen Benković, Michelle Spiering, Benković Stephen J   +2 more
exaly   +3 more sources

ATP is dispensable for E. coli DNA replication and eukaryotic helicase activity [PDF]

Nature Communications
Adenosine triphosphate (ATP) hydrolysis is the main cellular source of energy used to drive biochemical reactions that are otherwise energetically unfavourable.
Richard R. Spinks, Aleksa Lakic, Celine Kelso, Slobodan Jergic, Olga Yurieva, Zhi-Qiang Xu, Michael E. O’Donnell, Nicholas E. Dixon, Antoine M. van Oijen, Jacob S. Lewis, Lisanne M. Spenkelink   +10 more
doaj   +2 more sources

Torsion is a dynamic regulator of DNA replication stalling and reactivation [PDF]

Nature Communications
DNA’s helical structure necessitates replisome rotation relative to DNA during replication, creating inevitable topological challenges. How replication generates and overcomes torsional stress remains unclear.
Xiaomeng Jia, Xiang Gao, Shuming Zhang, James T. Inman, Yifeng Hong, Anupam Singh, Fahad Rashid, James M. Berger, Smita S. Patel, Michelle D. Wang   +9 more
doaj   +2 more sources

USP37 prevents unscheduled replisome unloading through MCM complex deubiquitination [PDF]

Nature Communications
The CMG helicase (CDC45-MCM2-7-GINS) unwinds DNA as a component of eukaryotic replisomes. Replisome (dis)assembly is tightly coordinated with cell cycle progression to ensure genome stability.
Derek L. Bolhuis, Dalia Fleifel, Thomas Bonacci, Xianxi Wang, Brandon L. Mouery, Jeanette Gowen Cook, Nicholas G. Brown, Michael J. Emanuele   +7 more
doaj   +2 more sources

Viruses and the host replisome: discovering oncogenic mechanisms of small DNA tumor viruses [PDF]

Journal of Virology
Human papillomavirus (HPV) and Merkel cell polyomavirus (MCPyV) are DNA tumor viruses that cause human cancer. The mechanisms by which HPV and MCPyV oncoproteins induce genomic instability are not well defined.
Christopher D. Collins, Matthew Stefely, Kavi Prem Milan Mehta, Megan E. Spurgeon   +3 more
doaj   +2 more sources

USP37 prevents premature disassembly of stressed replisomes by TRAIP [PDF]

Nature Communications
The eukaryotic replisome, which consists of the CDC45-MCM2-7-GINS (CMG) helicase, replicative polymerases, and several accessory factors, sometimes encounters proteinaceous obstacles that threaten genome integrity.
Olga V. Kochenova, Giuseppina D’Alessandro, Domenic Pilger, Ernst Schmid, Sean L. Richards, Marcos Rios Garcia, Satpal S. Jhujh, Andrea Voigt, Vipul Gupta, Christopher J. Carnie, R. Alex Wu, Nadia Gueorguieva, Simon Lam, Grant S. Stewart, Johannes C. Walter, Stephen P. Jackson   +15 more
doaj   +2 more sources

The Fate of Two Unstoppable Trains After Arriving Destination: Replisome Disassembly During DNA Replication Termination

Frontiers in Cell and Developmental Biology, 2021
In eukaryotes, the perfect duplication of the chromosomes is executed by a dynamic molecular machine called the replisome. As a key step to finishing DNA replication, replisome disassembly is triggered by ubiquitylation of the MCM7 subunit of the ...
Yisui Xia
doaj   +1 more source

DNA replication machinery: Insights from in vitro single-molecule approaches

Computational and Structural Biotechnology Journal, 2021
The replisome is the multiprotein molecular machinery that replicates DNA. The replisome components work in precise coordination to unwind the double helix of the DNA and replicate the two strands simultaneously.
Rebeca Bocanegra, G.A. Ismael Plaza, Carlos R. Pulido, Borja Ibarra   +3 more
doaj   +1 more source