Results 31 to 40 of about 30,157 (159)
eLife, 2021 R-loops are a major source of genome instability associated with transcription-induced replication stress. However, how R-loops inherently impact replication fork progression is not understood.
Charanya Kumar +3 more
doaj +1 more source
Replisome trafficking in growing vegetative hyphae of Streptomyces coelicolor A3(2)
, 2011 We observed movies of replisome trafficking during Streptomyces coelicolor growth. A replisome(s) in the spore served as a replication center(s) until hyphae reached a certain length, when a tip-proximal replisome formed and moved at a fixed distance ...
Jolanta Zakrzewska-Czerwińska +11 more
core +4 more sources
A Proteomic Characterization of Factors Enriched at Nascent DNA Molecules
Cell Reports, 2013 DNA replication is facilitated by multiple factors that concentrate in the vicinity of replication forks. Here, we developed an approach that combines the isolation of proteins on nascent DNA chains with mass spectrometry (iPOND-MS), allowing a ...
Andres J. Lopez-Contreras +10 more
doaj +1 more source
Mechanisms of eukaryotic replisome disassembly
, 2020 DNA replication is a complex process that needs to be executed accurately before cell division in order to maintain genome integrity. DNA replication is divided into three main stages: initiation, elongation and termination.
Gambus, Agnieszka; id_orcid +1 more
core +1 more source
Independent and Stochastic Action of DNA Polymerases in the Replisome [PDF]
, 2017 It has been assumed that DNA synthesis by the leading- and lagging-strand polymerases in the replisome must be coordinated to avoid the formation of significant gaps in the nascent strands. Using real-time single-molecule analysis, we establish that
Stephen C. Kowalczykowski +5 more
core +1 more source
Single-Molecule Insights Into the Dynamics of Replicative Helicases
Frontiers in Molecular Biosciences, 2021 Helicases are molecular motors that translocate along single-stranded DNA and unwind duplex DNA. They rely on the consumption of chemical energy from nucleotide hydrolysis to drive their translocation.
Richard R. Spinks +7 more
doaj +1 more source
Chromosome organization shapes replisome dynamics inCaulobacter crescentus
, 2023 DNA replication in bacteria takes place on highly compacted chromosomes, where segregation, transcription, and repair must occur simultaneously. Within this dynamic environment, colocalization of sister replisomes has been observed in many bacterial ...
Badrinarayanan, Anjana +5 more
core +1 more source
Frequent exchange of the DNA polymerase during bacterial chromosome replication
eLife, 2017 The replisome is a multiprotein machine that carries out DNA replication. In Escherichia coli, a single pair of replisomes is responsible for duplicating the entire 4.6 Mbp circular chromosome. In vitro studies of reconstituted E.
Thomas R Beattie +6 more
doaj +1 more source
eLife, 2020 Sister chromatid cohesion essential for mitotic chromosome segregation is thought to involve the co-entrapment of sister DNAs within cohesin rings. Although cohesin can load onto chromosomes throughout the cell cycle, it only builds cohesion during S ...
Madhusudhan Srinivasan +4 more
doaj +1 more source
mBio, 2023 Bacterial DNA methyltransferases (MTases) function in restriction modification systems, cell cycle control, and the regulation of gene expression. DnmA is a recently described DNA MTase that forms N6-methyladenosine at nonpalindromic 5′-GACGAG-3′ sites ...
Nicolas L. Fernandez +6 more
doaj +1 more source