Unwinding the Role of the CMG Helicase in Inborn Errors of Immunity. [PDF]
J Clin Immunol, 2023 Guilz NC, Ahn YO, Seo S, Mace EM.
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Tethering of SCF(Dia2) to the Replisome Promotes Efficient Ubiquitylation and Disassembly of the CMG Helicase. [PDF]
Curr Biol, 2015 Disassembly of the Cdc45-MCM-GINS (CMG) DNA helicase, which unwinds the parental DNA duplex at eukaryotic replication forks, is the key regulated step during replication termination but is poorly understood. In budding yeast, the F-box protein Dia2 drives ubiquitylation of the CMG helicase at the end of replication, leading to a disassembly pathway ...
Maculins T +3 more
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CDT1 inhibits CMG helicase in early S phase to separate origin licensing from DNA synthesis [PDF]
Molecular Cell, 2023 Joseph Yeeles +2 more
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Novel role of DONSON in CMG helicase assembly during vertebrate DNA replication initiation [PDF]
EMBO Journal, 2023 Yoshitami Hashimoto
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CMG helicase activity on G4-containing DNA templates. [PDF]
Methods Enzymol, 2022 G-quadruplexes (G4s) are non-canonical nucleic acid structures that form in G-rich regions of the genome and threaten genome stability by interfering with DNA replication. However, the underlying mechanisms are poorly understood. We have recently found that G4s can stall eukaryotic replication forks by blocking the progression of replicative DNA ...
Batra S, Devbhandari S, Remus D.
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The CMG helicase and cancer: a tumor "engine" and weakness with missing mutations. [PDF]
Oncogene, 2023 The replicative Cdc45-MCM-GINS (CMG) helicase is a large protein complex that functions in the DNA melting and unwinding steps as a component of replisomes during DNA replication in mammalian cells. Although the CMG performs this important role in cell growth, the CMG is not a simple bystander in cell cycle events.
Xiang S, Reed DR, Alexandrow MG.
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Myc and the Replicative CMG Helicase: The Creation and Destruction of Cancer: Myc Over-Activation of CMG Helicases Drives Tumorigenesis and Creates a Vulnerability in CMGs for Therapeutic Intervention. [PDF]
Bioessays, 2020 AbstractMyc‐driven tumorigenesis involves a non‐transcriptional role for Myc in over‐activating replicative Cdc45‐MCM‐GINS (CMG) helicases. Excessive stimulation of CMG helicases by Myc mismanages CMG function by diminishing the number of reserve CMGs necessary for fidelity of DNA replication and recovery from replicative stresses.
Reed DR, Alexandrow MG.
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CMG helicase can use ATPγS to unwind DNA: Implications for the rate-limiting step in the reaction mechanism [PDF]
Proceedings of the National Academy of Sciences of the United States of America, 2022 Michael O'Donnell +2 more
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DNA translocation by the CMG helicase: the helical inchworm model. [PDF]
Biochem Soc TransAbstract In all cells, hexameric helicases drive the unwinding of parental chromosomal DNA at replication forks to provide the single-stranded DNA templates required by replicative DNA polymerases. DNA unwinding proceeds via a steric exclusion mechanism in which the helicase encircles and translocates along one DNA strand while ...
Batra S +4 more
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DNSN-1 recruits GINS for CMG helicase assembly during DNA replication initiation in <i>Caenorhabditis elegans</i>. [PDF]
Science, 2023 INTRODUCTION: Eukaryotic chromosomes are duplicated by a molecular machine known as the replisome, the assembly of which is highly regulated to ensure that cells make a single copy of their genome during each cell cycle.
Xia Y +7 more
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