Results 41 to 50 of about 26,517 (143)
MCM7 Ubiquitination Regulates CMG Helicase Disassembly In Human [PDF]
, 2021 Abstract The disassembly of the replisome plays an essential role in maintaining genome stability at the termination of DNA replication. However, the mechanism of replisome disassembly remains unknown in human. In this study, we screened E3 ligases and deubiquitinases (DUBs) for the ubiquitination of minichromosome maintenance protein (MCM) 7 ...
Qianqian Sun +7 more
openaire +2 more sources
Frontiers in Cell and Developmental Biology, 2021 In eukaryotes, the perfect duplication of the chromosomes is executed by a dynamic molecular machine called the replisome. As a key step to finishing DNA replication, replisome disassembly is triggered by ubiquitylation of the MCM7 subunit of the ...
Yisui Xia
doaj +1 more source
Structure of eukaryotic CMG helicase at a replication fork and implications to replisome architecture and origin initiation [PDF]
Proceedings of the National Academy of Sciences of the United States of America, 2017 Ruda de Luna Almeida Santos +2 more
exaly +2 more sources
The CMG DNA helicase and the core replisome
Current Opinion in Structural Biology, 2023 Eukaryotic DNA replication is performed by the replisome, a large and dynamic multi-protein machine endowed with the required enzymatic components for the synthesis of new DNA. Recent cryo-electron microscopy (cryoEM) analyses have revealed the conserved architecture of the core eukaryotic replisome, comprising the CMG (Cdc45-MCM-GINS) DNA helicase ...
openaire +2 more sources
Phosphorylation of CMG helicase and Tof1 is required for programmed fork arrest. [PDF]
Proc Natl Acad Sci U S A, 2016 Significance Programmed replication fork arrest (PFA) at specific terminator sites and the proteins that bind to these sites functionally interconnect replication, transcription, and recombination. PFA prevents collision between replication and transcription that can cause genome instability, promotes intrachromatid recombination at ...
Bastia D +8 more
europepmc +4 more sources
Cell Reports, 2019 Summary: Progression of DNA replication depends on the ability of the replisome complex to overcome nucleoprotein barriers. During eukaryotic replication, the CMG helicase translocates along the leading-strand template and unwinds the DNA double helix ...
Hazal B. Kose +3 more
doaj +1 more source
Replisome Preservation by a Single-Stranded DNA Gate in the CMG Helicase [PDF]
Biophysical Journal, 2018 ABSTRACT The eukaryotic replicative helicase CMG is assembled at replication origins and is thought to remain topologically closed until termination. Upon encountering a lesion, CMG must vacate a stalled fork to allow DNA repair. However, the fate of CMG under these stress conditions remains unclear.
Wasserman, Michael R. +3 more
openaire +2 more sources
Mechanism of DNA-protein crosslink bypass by CMG helicase
ABSTRACT DNA-protein crosslinks (DPCs) pose significant barriers to DNA replication and genome integrity. Previous studies have established that bypass of DPCs by the eukaryotic replicative helicase, CMG complex, is critical for DPC repair. We investigated the mechanism of leading-strand DPC bypass by CMG using purified components.
Kyaw MT +3 more
europepmc +2 more sources
Structure of the eukaryotic replicative CMG helicase suggests a pumpjack motion for translocation. [PDF]
Nat Struct Mol Biol, 2016 The CMG helicase is composed of Cdc45, Mcm2-7 and GINS. Here we report the structure of the S. cerevisiae CMG determined by cryo-EM at a resolution of 3.7–4.8 Å. The structure reveals that GINS and Cdc45 scaffold the N-tier of the helicase while enabling motion of the AAA+ C-tier.
Yuan Z +6 more
europepmc +5 more sources
DDK regulates replication initiation by controlling the multiplicity of Cdc45-GINS binding to Mcm2-7
eLife, 2021 The committed step of eukaryotic DNA replication occurs when the pairs of Mcm2-7 replicative helicases that license each replication origin are activated. Helicase activation requires the recruitment of Cdc45 and GINS to Mcm2-7, forming Cdc45-Mcm2-7-GINS
Lorraine De Jesús-Kim +5 more
doaj +1 more source