Results 61 to 70 of about 2,048 (142)

Mcm10 promotes rapid isomerization of CMG-DNA for replisome bypass of lagging strand DNA blocks

open access: yeseLife, 2017
Replicative helicases in all cell types are hexameric rings that unwind DNA by steric exclusion in which the helicase encircles the tracking strand only and excludes the other strand from the ring.
Lance D Langston   +7 more
doaj   +1 more source

Myc-driven chromatin accessibility regulates Cdc45 assembly into CMG helicases [PDF]

open access: yesCommunications Biology, 2019
Abstract Myc-driven tumorigenesis involves a non-transcriptional role for Myc in over-activating replication origins. We show here that the mechanism underlying this process involves a direct role for Myc in activation of Cdc45-MCM-GINS (CMG) helicases at Myc-targeted sites.
Nepon-Sixt, Brook S.   +2 more
openaire   +2 more sources

Structure of DNA-CMG-Pol epsilon elucidates the roles of the non-catalytic polymerase modules in the eukaryotic replisome

open access: yesNature Communications, 2018
Eukaryotic origin firing depends on assembly of the Cdc45-MCM-GINS (CMG) helicase, which requires the leading-strand polymerase Pol ɛ. Here the authors present a structural analysis of a CMG Pol ɛ on a DNA fork, providing insight on the steps leading ...
Panchali Goswami   +11 more
doaj   +1 more source

Helicase binding to DnaI exposes a cryptic DNA-binding site during helicase loading in Bacillus subtilis [PDF]

open access: yes, 1981
The Bacillus subtilis DnaI, DnaB and DnaD proteins load the replicative ring helicase DnaC onto DNA during priming of DNA replication. Here we show that DnaI consists of a C-terminal domain (Cd) with ATPase and DNA-binding activities and an N-terminal ...
Shi, Wei   +999 more
core   +1 more source

Cryo-EM studies of CMG translocation and cohesin loading [PDF]

open access: yes, 2021
Before mitosis, the genome must be replicated in the parental cell and sister chromatids must be tethered to ensure accurate division of genes between daughter cells. Within the replisome, DNA unwinding is facilitated by the CMG helicase which contains a
Eickhoff, Carl Patrik
core   +1 more source

Cdt1 inhibits CMG helicase in early S phase to separate origin licensing from DNA synthesis. Ratnayeke et al.

open access: yes, 2022
Image data for manuscript submitted to Molecular Cell, Ratnayeke et al, "Cdt1 inhibits CMG helicase in early S phase to separate origin licensing from DNA synthesis ...
Meyer, T
core   +2 more sources

In the absence of ATPase activity, pre-RC formation is blocked prior to MCM2–7 hexamer dimerization [PDF]

open access: yes, 2013
26.03.14 KB.
Brill, S   +8 more
core   +1 more source

DNA polymerase ε-dependent modulation of the pausing property of the CMG helicase at the barrier. [PDF]

open access: yesGenes Dev, 2018
The proper pausing of replication forks at barriers on chromosomes is important for genome integrity. However, the detailed mechanism underlying this process has not been well elucidated. Here, we successfully reconstituted fork-pausing reactions from purified yeast proteins on templates that had binding sites for the LacI, LexA, and/or Fob1 proteins ...
Hizume K   +4 more
europepmc   +4 more sources

Multiple UBX proteins reduce the ubiquitin threshold of the mammalian p97-UFD1-NPL4 unfoldase

open access: yeseLife, 2022
The p97/Cdc48 ATPase and its ubiquitin receptors Ufd1-Npl4 are essential to unfold ubiquitylated proteins in many areas of eukaryotic cell biology.
Ryo Fujisawa   +2 more
doaj   +1 more source

Rad53 checkpoint kinase regulation of DNA replication fork rate via Mrc1 phosphorylation

open access: yeseLife, 2021
The Rad53 DNA checkpoint protein kinase plays multiple roles in the budding yeast cell response to DNA replication stress. Key amongst these is its enigmatic role in safeguarding DNA replication forks.
Allison W McClure, John FX Diffley
doaj   +1 more source

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