Results 71 to 80 of about 2,048 (142)

Expanding Phenotype of GINS1 Deficiency: A Case Report and Review of the Literature

Clinical Genetics, Volume 109, Issue 6, Page 1076-1080, June 2026.
The authors present a novel case and review of individuals with GINS1 deficiency, causing severe growth restriction and combined immunodeficiency. Only the ninth case of this ultrarare disorder, it highlights the role of these variants in disease, glaucoma as a feature, and the possibility of immunodeficiency without infections in affected individuals.
Michael P. Mackley, Rae Brager, Hannah Geddie, Vicky Breakey, Rebecca Hough, Dimitrios J. Stavropoulos, Vanda McNiven   +6 more
wiley   +1 more source

Modulation of MCM Helicase activity [PDF]

, 2011
The minichromosome maintenance (MCM) proteins are essential for the initiation and progression of DNA replication. MCMs are believed to function as the replicative helicase in archaea and eukaryotes.
Satti, Rabab
core  

Cryo-EM structures of the eukaryotic replicative helicase bound to a translocation substrate

Nature Communications, 2016
The Cdc45-MCM-GINS (CMG) helicase unwinds DNA during replication, a process that requires the ATPase-dependent activity of the MCM complex. Using cryo-EM reconstructions of the CMG complex in different conformations, the authors propose a model where the
Ferdos Abid Ali, Ludovic Renault, Julian Gannon, Hailey L. Gahlon, Abhay Kotecha, Jin Chuan Zhou, David Rueda, Alessandro Costa   +7 more
doaj   +1 more source

Overexpression of CDT1 inhibits cell cycle progression at S phase by interacting with the mini‐chromosome maintenance complex and causes DNA damage

FEBS Open Bio, Volume 16, Issue 2, Page 352-364, February 2026.
CDT1 is an essential protein for DNA replication licensing that loads the MCM complex, the eukaryotic replicative DNA helicase, onto replication origins. Overexpression of CDT1 induces cell cycle arrest at the S phase. Here we showed CDT1 inhibits the progression of replication forks by interacting with the MCM complex, leading to the stalling and ...
Takashi Tsuyama, Nonoka Takayama, Rina Tanaka, Yuuki Arai, Yohko Yamaguchi, Yuko Nawata, Yutaro Azuma, Shusuke Tada   +7 more
wiley   +1 more source

License to Replicate: Mechanisms of Licensing Eukaryotic Origins for DNA Replication

BioEssays, Volume 48, Issue 1, January 2026.
Origin licensing constitutes the first step in initiating eukaryotic genome replication. Licensed origins are marked by an MCM double hexamer complex. This article reviews the molecular mechanisms of MCM double hexamer loading in yeast and higher eukaryotes, based on insights gained from biochemical reconstitution and structural analyses.
Victoria Frisbie, Franziska Bleichert
wiley   +1 more source

Dimerization of Firing Factors for Replication Origin Activation in Eukaryotes: A Crucial Process for Simultaneous Assembly of Bidirectional Replication Forks?

Biology, 2022
Controlling the activity of the heterohexameric Mcm2–7 replicative helicase is crucial for regulation of replication origin activity in eukaryotes. Because bidirectional replication forks are generated from every replication origin, when origins are ...
Seiji Tanaka, Shiho Ogawa
doaj   +1 more source

Topoisomerase I Inhibition in ETV4‐overexpressed Non‐Small Cell Lung Cancer Promotes Replication and Transcription Mediated R‐Loop Accumulation and DNA Damage

Advanced Science, Volume 12, Issue 35, September 18, 2025.
The oncogenic ETS factor ETV4 exerts a pleiotropic control over DNA replication both in a transcription‐dependent and ‐independent fashion in NSCLC cells. High‐ETV4 expression leads to R‐loop formation and DNA damage in response to TOP1 inhibition.
Jiaxi Zhang, Yan Wang, Shanhu Cao, Shelly M. Xie, Bei Liu, Yimeng Li, Yuqi Hou, Xue Meng, Mingzhu Ruan, Di Bu, Jia Kang, Ruxin Li, Lei Lou, Juan Wang, Lingxiao Xing   +14 more
wiley   +1 more source

Compact Origins and Where to Find Them: ORC's Guide to Genome‐Wide Licensing

BioEssays, Volume 47, Issue 7, July 2025.
Origin licensing is a key requirement for any eukaryotic cell to initiate DNA replication in S‐phase. Here, we review the most recent findings in the orchestrated action of the origin recognition complex (ORC) to sequentially load two MCM2‐7 helicases onto DNA with a focus on origin architecture and sequencing‐based methods.
Christian Speck, Luitpold Maximilian Reuter   +1 more
wiley   +1 more source

Macromolecular crystallography at Elettra: current and future perspectives

Journal of Synchrotron Radiation, Volume 32, Issue 3, Page 757-765, May 2025.
The XRD2 beamline at Elettra‐Sincrotrone Trieste has been in operation since 2018 and is dedicated to macromolecular crystallography for both academic and industrial research, a role partially fulfilled, before 2018, by XRD1, a general‐purpose diffraction beamline.
Raghurama P. Hegde, Nicola Demitri, Annie Héroux, Alessandro Olivo, Giorgio Bais, Michele Cianci, Paola Storici, Dan-George Dumitrescu, Nishant Kumar Varshney, Balasubramanian Gopal, D. D. Sarma, Lisa Vaccari, Silvia Onesti, Maurizio Polentarutti   +13 more
wiley   +1 more source

Structure of the Saccharolobus solfataricus GINS tetramer

Acta Crystallographica Section F, Volume 81, Issue 5, Page 207-215, May 2025.
Archaeal organisms possess Cdc45 and GINS homologs that are likely to serve similar functional roles, but their structural interactions with the MCM helicase and hence their mechanism of MCM activation are not as well understood as for their eukaryotic counterparts. We present the crystal structure of the S.
Srihari Shankar, Eric J. Enemark
wiley   +1 more source